Experiment: Gelatin (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2416 and Shew_2417 are separated by 131 nucleotides Shew_2417 and Shew_2418 are separated by 79 nucleotides Shew_2418 and Shew_2419 are separated by 173 nucleotides
Shew_2416: Shew_2416 - chorismate synthase (RefSeq), at 2,836,447 to 2,837,541
_2416
Shew_2417: Shew_2417 - N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase (RefSeq), at 2,837,673 to 2,838,617
_2417
Shew_2418: Shew_2418 - hypothetical protein (RefSeq), at 2,838,697 to 2,839,224
_2418
Shew_2419: Shew_2419 - phosphohistidine phosphatase, SixA (RefSeq), at 2,839,398 to 2,839,868
_2419
Position (kb)
2837
2838
2839 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2837.093 kb on - strand, within Shew_2416 at 2837.372 kb on + strand, within Shew_2416 at 2837.478 kb on - strand at 2838.019 kb on + strand, within Shew_2417 at 2838.468 kb on + strand, within Shew_2417 at 2838.476 kb on - strand, within Shew_2417 at 2838.839 kb on - strand, within Shew_2418 at 2839.042 kb on - strand, within Shew_2418 at 2839.042 kb on - strand, within Shew_2418 at 2839.131 kb on + strand, within Shew_2418 at 2839.258 kb on + strand at 2839.528 kb on - strand, within Shew_2419 at 2839.567 kb on + strand, within Shew_2419
Per-strain Table
Position Strand Gene LocusTag Fraction Gelatin (C) remove 2,837,093 - Shew_2416 0.59 -3.7 2,837,372 + Shew_2416 0.84 -2.0 2,837,478 - -4.2 2,838,019 + Shew_2417 0.37 +0.2 2,838,468 + Shew_2417 0.84 -0.4 2,838,476 - Shew_2417 0.85 -0.5 2,838,839 - Shew_2418 0.27 -0.2 2,839,042 - Shew_2418 0.65 -0.7 2,839,042 - Shew_2418 0.65 +0.3 2,839,131 + Shew_2418 0.82 +0.2 2,839,258 + +0.4 2,839,528 - Shew_2419 0.28 +0.3 2,839,567 + Shew_2419 0.36 +0.4
Or see this region's nucleotide sequence