Experiment: Gelatin (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2358 and Shew_2359 are separated by 69 nucleotides Shew_2359 and Shew_2360 are separated by 161 nucleotides Shew_2360 and Shew_2361 overlap by 4 nucleotides
Shew_2358: Shew_2358 - peptidase M24 (RefSeq), at 2,758,272 to 2,759,591
_2358
Shew_2359: Shew_2359 - uracil-DNA glycosylase superfamily protein (RefSeq), at 2,759,661 to 2,760,251
_2359
Shew_2360: Shew_2360 - D-hydroxyproline dehydrogenase (from data) , at 2,760,413 to 2,761,753
_2360
Shew_2361: Shew_2361 - proline racemase (RefSeq), at 2,761,750 to 2,762,790
_2361
Position (kb)
2760
2761
2762 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2759.728 kb on + strand, within Shew_2359 at 2760.280 kb on + strand at 2760.582 kb on + strand, within Shew_2360 at 2760.606 kb on - strand, within Shew_2360 at 2760.651 kb on + strand, within Shew_2360 at 2760.685 kb on + strand, within Shew_2360 at 2760.995 kb on - strand, within Shew_2360 at 2761.155 kb on + strand, within Shew_2360 at 2761.181 kb on + strand, within Shew_2360 at 2761.461 kb on - strand, within Shew_2360 at 2761.701 kb on + strand at 2761.701 kb on + strand at 2762.211 kb on - strand, within Shew_2361 at 2762.366 kb on + strand, within Shew_2361 at 2762.407 kb on + strand, within Shew_2361 at 2762.433 kb on + strand, within Shew_2361
Per-strain Table
Position Strand Gene LocusTag Fraction Gelatin (C) remove 2,759,728 + Shew_2359 0.11 -0.7 2,760,280 + -0.1 2,760,582 + Shew_2360 0.13 -1.1 2,760,606 - Shew_2360 0.14 -0.8 2,760,651 + Shew_2360 0.18 -2.4 2,760,685 + Shew_2360 0.20 -2.5 2,760,995 - Shew_2360 0.43 -1.5 2,761,155 + Shew_2360 0.55 -2.0 2,761,181 + Shew_2360 0.57 -2.0 2,761,461 - Shew_2360 0.78 -2.8 2,761,701 + -1.6 2,761,701 + -1.6 2,762,211 - Shew_2361 0.44 -3.3 2,762,366 + Shew_2361 0.59 -1.2 2,762,407 + Shew_2361 0.63 -2.0 2,762,433 + Shew_2361 0.66 -2.0
Or see this region's nucleotide sequence