Experiment: Gelatin (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0322 and dapF are separated by 37 nucleotides dapF and Shew_0324 are separated by 8 nucleotides Shew_0324 and Shew_0325 are separated by 7 nucleotides Shew_0325 and Shew_0326 overlap by 8 nucleotides
Shew_0322: Shew_0322 - diaminopimelate decarboxylase (RefSeq), at 368,267 to 369,511
_0322
Shew_0323: dapF - diaminopimelate epimerase (RefSeq), at 369,549 to 370,388
dapF
Shew_0324: Shew_0324 - hypothetical protein (RefSeq), at 370,397 to 371,023
_0324
Shew_0325: Shew_0325 - tyrosine recombinase XerC (RefSeq), at 371,031 to 371,924
_0325
Shew_0326: Shew_0326 - HAD family hydrolase (RefSeq), at 371,917 to 372,639
_0326
Position (kb) 370
371
372 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 369.403 kb on - strand at 369.505 kb on + strand at 369.505 kb on + strand at 370.489 kb on + strand, within Shew_0324 at 370.533 kb on + strand, within Shew_0324 at 370.666 kb on + strand, within Shew_0324 at 371.311 kb on + strand, within Shew_0325 at 371.516 kb on + strand, within Shew_0325 at 371.664 kb on + strand, within Shew_0325 at 371.742 kb on - strand, within Shew_0325 at 371.805 kb on + strand, within Shew_0325 at 371.837 kb on + strand at 371.837 kb on + strand at 371.845 kb on - strand at 371.874 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gelatin (C) remove 369,403 - -1.5 369,505 + +0.4 369,505 + +0.3 370,489 + Shew_0324 0.15 -0.7 370,533 + Shew_0324 0.22 -3.4 370,666 + Shew_0324 0.43 -2.5 371,311 + Shew_0325 0.31 -1.4 371,516 + Shew_0325 0.54 -1.3 371,664 + Shew_0325 0.71 -2.1 371,742 - Shew_0325 0.80 -1.6 371,805 + Shew_0325 0.87 -1.1 371,837 + -1.1 371,837 + -1.3 371,845 - -0.6 371,874 + -2.9
Or see this region's nucleotide sequence