Experiment: Light Dark second sampling rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_2019 and infB are separated by 82 nucleotides infB and Synpcc7942_2021 are separated by 150 nucleotides Synpcc7942_2021 and nusA overlap by 4 nucleotides
Synpcc7942_2019: Synpcc7942_2019 - hypothetical protein, at 2,085,893 to 2,086,762
_2019
Synpcc7942_2020: infB - translation initiation factor IF-2, at 2,086,845 to 2,089,937
infB
Synpcc7942_2021: Synpcc7942_2021 - hypothetical protein, at 2,090,088 to 2,090,381
_2021
Synpcc7942_2022: nusA - transcription elongation factor NusA, at 2,090,378 to 2,091,703
nusA
Position (kb)
2086
2087
2088
2089
2090 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2085.852 kb on - strand at 2085.883 kb on + strand at 2085.885 kb on - strand at 2085.899 kb on - strand at 2085.907 kb on + strand at 2085.913 kb on - strand at 2085.913 kb on - strand at 2086.026 kb on + strand, within Synpcc7942_2019 at 2086.026 kb on + strand at 2086.155 kb on - strand, within Synpcc7942_2019 at 2086.184 kb on + strand, within Synpcc7942_2019 at 2086.184 kb on + strand, within Synpcc7942_2019 at 2086.192 kb on - strand, within Synpcc7942_2019 at 2086.231 kb on - strand, within Synpcc7942_2019 at 2086.252 kb on - strand, within Synpcc7942_2019 at 2086.273 kb on + strand, within Synpcc7942_2019 at 2086.273 kb on + strand, within Synpcc7942_2019 at 2086.301 kb on + strand, within Synpcc7942_2019 at 2086.438 kb on + strand, within Synpcc7942_2019 at 2086.517 kb on - strand, within Synpcc7942_2019 at 2086.527 kb on + strand, within Synpcc7942_2019 at 2086.585 kb on - strand, within Synpcc7942_2019 at 2086.590 kb on - strand, within Synpcc7942_2019 at 2086.594 kb on + strand, within Synpcc7942_2019 at 2086.697 kb on - strand at 2086.775 kb on + strand at 2086.783 kb on - strand at 2086.840 kb on + strand at 2086.842 kb on + strand at 2086.882 kb on - strand at 2090.143 kb on - strand, within Synpcc7942_2021 at 2090.347 kb on + strand, within Synpcc7942_2021 at 2090.361 kb on + strand at 2090.361 kb on + strand at 2090.375 kb on + strand at 2090.397 kb on + strand at 2090.405 kb on - strand at 2090.424 kb on + strand at 2090.432 kb on - strand at 2090.448 kb on + strand at 2090.468 kb on - strand at 2090.478 kb on + strand at 2090.535 kb on + strand, within nusA at 2090.543 kb on - strand, within nusA
Per-strain Table
Position Strand Gene LocusTag Fraction Light Dark second sampling rep2 remove 2,085,852 - -0.4 2,085,883 + +0.7 2,085,885 - +0.1 2,085,899 - +0.5 2,085,907 + -0.2 2,085,913 - -0.3 2,085,913 - -0.5 2,086,026 + Synpcc7942_2019 0.15 -1.0 2,086,026 + -1.7 2,086,155 - Synpcc7942_2019 0.30 +0.9 2,086,184 + Synpcc7942_2019 0.33 -2.4 2,086,184 + Synpcc7942_2019 0.33 -1.8 2,086,192 - Synpcc7942_2019 0.34 -1.9 2,086,231 - Synpcc7942_2019 0.39 -2.0 2,086,252 - Synpcc7942_2019 0.41 -1.0 2,086,273 + Synpcc7942_2019 0.44 -4.1 2,086,273 + Synpcc7942_2019 0.44 -1.8 2,086,301 + Synpcc7942_2019 0.47 -3.5 2,086,438 + Synpcc7942_2019 0.63 -1.5 2,086,517 - Synpcc7942_2019 0.72 -0.8 2,086,527 + Synpcc7942_2019 0.73 -1.5 2,086,585 - Synpcc7942_2019 0.80 -0.3 2,086,590 - Synpcc7942_2019 0.80 -2.8 2,086,594 + Synpcc7942_2019 0.81 -1.4 2,086,697 - -1.2 2,086,775 + -0.0 2,086,783 - -0.3 2,086,840 + +0.1 2,086,842 + -0.8 2,086,882 - -1.9 2,090,143 - Synpcc7942_2021 0.19 -0.2 2,090,347 + Synpcc7942_2021 0.88 -3.9 2,090,361 + -0.3 2,090,361 + -0.6 2,090,375 + -0.6 2,090,397 + +0.5 2,090,405 - -0.7 2,090,424 + -2.2 2,090,432 - -2.5 2,090,448 + -1.6 2,090,468 - -1.8 2,090,478 + -2.5 2,090,535 + nusA Synpcc7942_2022 0.12 -4.8 2,090,543 - nusA Synpcc7942_2022 0.12 +0.2
Or see this region's nucleotide sequence