Experiment: Light Dark second sampling rep2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_0438 and chlM are separated by 115 nucleotides chlM and nagA are separated by 45 nucleotides nagA and purE are separated by 49 nucleotides purE and amt1 are separated by 266 nucleotides
Synpcc7942_0438: Synpcc7942_0438 - hypothetical protein, at 427,989 to 428,495
_0438
Synpcc7942_0439: chlM - Mg-protoporphyrin IX methyl transferase, at 428,611 to 429,300
chlM
Synpcc7942_0440: nagA - N-acetylglucosamine 6-phosphate deacetylase, at 429,346 to 430,473
nagA
Synpcc7942_0441: purE - phosphoribosylaminoimidazole carboxylase, catalytic subunit, at 430,523 to 431,191
purE
Synpcc7942_0442: amt1 - ammonium transporter, at 431,458 to 432,951
amt1
Position (kb) 429
430
431 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 428.460 kb on - strand at 429.296 kb on + strand at 429.343 kb on + strand at 429.351 kb on - strand at 429.352 kb on - strand at 429.354 kb on + strand at 429.362 kb on - strand at 430.551 kb on - strand at 430.570 kb on + strand at 430.570 kb on + strand at 430.587 kb on + strand at 430.611 kb on + strand, within purE at 430.618 kb on - strand, within purE at 430.627 kb on - strand, within purE at 431.217 kb on + strand at 431.225 kb on + strand at 431.233 kb on - strand at 431.306 kb on + strand at 431.336 kb on - strand at 431.404 kb on + strand at 431.412 kb on + strand at 431.420 kb on - strand at 431.439 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Light Dark second sampling rep2 remove 428,460 - +0.1 429,296 + -1.0 429,343 + -0.1 429,351 - -2.3 429,352 - +0.3 429,354 + -0.2 429,362 - -0.1 430,551 - -1.1 430,570 + -0.3 430,570 + -0.9 430,587 + -0.1 430,611 + purE Synpcc7942_0441 0.13 +0.5 430,618 - purE Synpcc7942_0441 0.14 -0.1 430,627 - purE Synpcc7942_0441 0.16 -0.2 431,217 + +0.6 431,225 + -0.8 431,233 - -0.2 431,306 + -3.4 431,336 - -0.6 431,404 + -1.6 431,412 + -1.4 431,420 - -0.8 431,439 + -1.5
Or see this region's nucleotide sequence