Experiment: R2A with Chloramphenicol 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS20165 and CA265_RS20170 are separated by 5 nucleotides CA265_RS20170 and CA265_RS20175 overlap by 4 nucleotides CA265_RS20175 and CA265_RS20180 are separated by 10 nucleotides CA265_RS20180 and CA265_RS20185 overlap by 14 nucleotides
CA265_RS20165: CA265_RS20165 - phosphomethylpyrimidine synthase ThiC, at 4,985,037 to 4,986,920
_RS20165
CA265_RS20170: CA265_RS20170 - hypothetical protein, at 4,986,926 to 4,987,540
_RS20170
CA265_RS20175: CA265_RS20175 - thiamine-phosphate diphosphorylase, at 4,987,537 to 4,988,175
_RS20175
CA265_RS20180: CA265_RS20180 - thiazole synthase, at 4,988,186 to 4,988,956
_RS20180
CA265_RS20185: CA265_RS20185 - 2-iminoacetate synthase ThiH, at 4,988,943 to 4,990,061
_RS20185
Position (kb)
4987
4988
4989 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4986.551 kb on + strand, within CA265_RS20165 at 4986.641 kb on - strand, within CA265_RS20165 at 4986.662 kb on + strand, within CA265_RS20165 at 4986.961 kb on + strand at 4987.060 kb on + strand, within CA265_RS20170 at 4987.114 kb on + strand, within CA265_RS20170 at 4987.115 kb on - strand, within CA265_RS20170 at 4987.115 kb on - strand, within CA265_RS20170 at 4987.133 kb on - strand, within CA265_RS20170 at 4987.207 kb on - strand, within CA265_RS20170 at 4987.233 kb on + strand, within CA265_RS20170 at 4987.234 kb on - strand, within CA265_RS20170 at 4987.263 kb on - strand, within CA265_RS20170 at 4987.263 kb on - strand, within CA265_RS20170 at 4987.307 kb on + strand, within CA265_RS20170 at 4987.474 kb on - strand, within CA265_RS20170 at 4987.560 kb on + strand at 4987.561 kb on - strand at 4987.593 kb on - strand at 4987.611 kb on - strand, within CA265_RS20175 at 4987.670 kb on + strand, within CA265_RS20175 at 4987.728 kb on + strand, within CA265_RS20175 at 4987.768 kb on + strand, within CA265_RS20175 at 4987.787 kb on + strand, within CA265_RS20175 at 4987.848 kb on + strand, within CA265_RS20175 at 4987.900 kb on + strand, within CA265_RS20175 at 4987.901 kb on - strand, within CA265_RS20175 at 4987.958 kb on + strand, within CA265_RS20175 at 4987.995 kb on - strand, within CA265_RS20175 at 4988.076 kb on + strand, within CA265_RS20175 at 4988.080 kb on + strand, within CA265_RS20175 at 4988.081 kb on - strand, within CA265_RS20175 at 4988.133 kb on - strand at 4988.185 kb on + strand at 4988.186 kb on - strand at 4988.186 kb on - strand at 4988.344 kb on + strand, within CA265_RS20180 at 4988.345 kb on - strand, within CA265_RS20180 at 4988.380 kb on - strand, within CA265_RS20180 at 4988.570 kb on + strand, within CA265_RS20180 at 4988.571 kb on - strand, within CA265_RS20180 at 4988.593 kb on - strand, within CA265_RS20180 at 4988.613 kb on + strand, within CA265_RS20180 at 4988.613 kb on + strand, within CA265_RS20180 at 4988.613 kb on + strand, within CA265_RS20180 at 4988.613 kb on + strand, within CA265_RS20180 at 4988.692 kb on + strand, within CA265_RS20180 at 4988.693 kb on - strand, within CA265_RS20180 at 4988.737 kb on - strand, within CA265_RS20180 at 4988.737 kb on - strand, within CA265_RS20180 at 4988.845 kb on + strand, within CA265_RS20180 at 4988.893 kb on + strand at 4988.974 kb on - strand at 4989.026 kb on - strand at 4989.045 kb on - strand at 4989.110 kb on + strand, within CA265_RS20185 at 4989.122 kb on - strand, within CA265_RS20185 at 4989.168 kb on + strand, within CA265_RS20185 at 4989.168 kb on + strand, within CA265_RS20185 at 4989.169 kb on - strand, within CA265_RS20185 at 4989.169 kb on - strand, within CA265_RS20185 at 4989.169 kb on - strand, within CA265_RS20185 at 4989.169 kb on - strand, within CA265_RS20185
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloramphenicol 0.005 mg/ml remove 4,986,551 + CA265_RS20165 0.80 -0.6 4,986,641 - CA265_RS20165 0.85 -0.0 4,986,662 + CA265_RS20165 0.86 -0.3 4,986,961 + -0.0 4,987,060 + CA265_RS20170 0.22 -1.5 4,987,114 + CA265_RS20170 0.31 -0.9 4,987,115 - CA265_RS20170 0.31 +1.0 4,987,115 - CA265_RS20170 0.31 -0.6 4,987,133 - CA265_RS20170 0.34 +0.4 4,987,207 - CA265_RS20170 0.46 +0.6 4,987,233 + CA265_RS20170 0.50 -1.0 4,987,234 - CA265_RS20170 0.50 -0.7 4,987,263 - CA265_RS20170 0.55 -1.3 4,987,263 - CA265_RS20170 0.55 +0.8 4,987,307 + CA265_RS20170 0.62 +1.0 4,987,474 - CA265_RS20170 0.89 +0.4 4,987,560 + +1.1 4,987,561 - -0.5 4,987,593 - +0.1 4,987,611 - CA265_RS20175 0.12 -0.7 4,987,670 + CA265_RS20175 0.21 +0.5 4,987,728 + CA265_RS20175 0.30 -1.4 4,987,768 + CA265_RS20175 0.36 +0.2 4,987,787 + CA265_RS20175 0.39 -0.9 4,987,848 + CA265_RS20175 0.49 +0.5 4,987,900 + CA265_RS20175 0.57 -1.4 4,987,901 - CA265_RS20175 0.57 +0.6 4,987,958 + CA265_RS20175 0.66 -0.0 4,987,995 - CA265_RS20175 0.72 +1.4 4,988,076 + CA265_RS20175 0.84 -1.1 4,988,080 + CA265_RS20175 0.85 -1.2 4,988,081 - CA265_RS20175 0.85 -2.0 4,988,133 - -1.3 4,988,185 + +1.3 4,988,186 - +0.9 4,988,186 - -0.3 4,988,344 + CA265_RS20180 0.20 +0.7 4,988,345 - CA265_RS20180 0.21 -1.6 4,988,380 - CA265_RS20180 0.25 +0.4 4,988,570 + CA265_RS20180 0.50 -0.7 4,988,571 - CA265_RS20180 0.50 -1.1 4,988,593 - CA265_RS20180 0.53 +0.1 4,988,613 + CA265_RS20180 0.55 -0.4 4,988,613 + CA265_RS20180 0.55 +0.5 4,988,613 + CA265_RS20180 0.55 -0.9 4,988,613 + CA265_RS20180 0.55 +0.1 4,988,692 + CA265_RS20180 0.66 -0.9 4,988,693 - CA265_RS20180 0.66 +0.7 4,988,737 - CA265_RS20180 0.71 -1.2 4,988,737 - CA265_RS20180 0.71 -0.9 4,988,845 + CA265_RS20180 0.85 +0.1 4,988,893 + +0.0 4,988,974 - -1.3 4,989,026 - +0.6 4,989,045 - -0.9 4,989,110 + CA265_RS20185 0.15 +0.7 4,989,122 - CA265_RS20185 0.16 +2.5 4,989,168 + CA265_RS20185 0.20 +0.1 4,989,168 + CA265_RS20185 0.20 +0.1 4,989,169 - CA265_RS20185 0.20 +0.1 4,989,169 - CA265_RS20185 0.20 -0.8 4,989,169 - CA265_RS20185 0.20 +0.5 4,989,169 - CA265_RS20185 0.20 -0.3
Or see this region's nucleotide sequence