Experiment: Cisplatin 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_00675 and AO353_00680 are separated by 322 nucleotides AO353_00680 and AO353_00685 are separated by 67 nucleotides AO353_00685 and AO353_00690 are separated by 146 nucleotides AO353_00690 and AO353_00695 are separated by 3 nucleotides
AO353_00675: AO353_00675 - recombination protein RecR, at 142,994 to 143,596
_00675
AO353_00680: AO353_00680 - nucleoid-associated protein, at 143,919 to 144,257
_00680
AO353_00685: AO353_00685 - DNA polymerase III subunit gamma/tau, at 144,325 to 146,433
_00685
AO353_00690: AO353_00690 - amino acid ABC transporter substrate-binding protein, at 146,580 to 147,338
_00690
AO353_00695: AO353_00695 - diguanylate cyclase, at 147,342 to 149,042
_00695
Position (kb)
144
145
146
147 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 143.354 kb on + strand, within AO353_00675 at 143.354 kb on + strand, within AO353_00675 at 143.354 kb on + strand, within AO353_00675 at 143.354 kb on + strand, within AO353_00675 at 143.354 kb on + strand, within AO353_00675 at 143.355 kb on - strand, within AO353_00675 at 143.355 kb on - strand, within AO353_00675 at 143.355 kb on - strand, within AO353_00675 at 143.355 kb on - strand, within AO353_00675 at 143.515 kb on + strand, within AO353_00675 at 143.516 kb on - strand, within AO353_00675 at 143.614 kb on + strand at 143.614 kb on + strand at 143.614 kb on + strand at 143.614 kb on + strand at 143.615 kb on - strand at 143.615 kb on - strand at 143.615 kb on - strand at 143.632 kb on + strand at 143.633 kb on - strand at 143.633 kb on - strand at 143.634 kb on + strand at 143.634 kb on + strand at 143.748 kb on + strand at 143.748 kb on + strand at 143.749 kb on - strand at 143.780 kb on - strand at 143.896 kb on - strand at 144.141 kb on - strand, within AO353_00680 at 144.276 kb on - strand at 144.276 kb on - strand at 144.276 kb on - strand at 144.276 kb on - strand at 144.324 kb on - strand at 144.324 kb on - strand at 144.324 kb on - strand at 144.324 kb on - strand at 144.326 kb on + strand at 146.512 kb on + strand at 146.521 kb on + strand at 146.529 kb on + strand at 146.530 kb on - strand at 146.579 kb on + strand at 146.721 kb on + strand, within AO353_00690 at 146.721 kb on + strand, within AO353_00690 at 146.721 kb on + strand, within AO353_00690 at 146.836 kb on - strand, within AO353_00690 at 146.874 kb on + strand, within AO353_00690 at 146.874 kb on + strand, within AO353_00690 at 146.874 kb on + strand, within AO353_00690 at 146.875 kb on - strand, within AO353_00690 at 146.875 kb on - strand, within AO353_00690 at 146.984 kb on - strand, within AO353_00690 at 147.092 kb on + strand, within AO353_00690 at 147.100 kb on - strand, within AO353_00690 at 147.204 kb on + strand, within AO353_00690 at 147.204 kb on + strand, within AO353_00690 at 147.204 kb on + strand, within AO353_00690 at 147.204 kb on + strand, within AO353_00690 at 147.205 kb on - strand, within AO353_00690 at 147.205 kb on - strand, within AO353_00690 at 147.212 kb on + strand, within AO353_00690 at 147.301 kb on - strand at 147.301 kb on - strand at 147.337 kb on - strand at 147.337 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.006 mg/ml remove 143,354 + AO353_00675 0.60 -2.1 143,354 + AO353_00675 0.60 -1.1 143,354 + AO353_00675 0.60 -2.5 143,354 + AO353_00675 0.60 -4.1 143,354 + AO353_00675 0.60 -0.1 143,355 - AO353_00675 0.60 -0.1 143,355 - AO353_00675 0.60 -4.0 143,355 - AO353_00675 0.60 -1.7 143,355 - AO353_00675 0.60 -1.7 143,515 + AO353_00675 0.86 -3.3 143,516 - AO353_00675 0.87 -3.1 143,614 + -1.1 143,614 + -2.1 143,614 + -2.7 143,614 + -2.7 143,615 - -2.7 143,615 - -2.5 143,615 - -0.1 143,632 + -3.3 143,633 - +0.9 143,633 - -1.1 143,634 + -2.1 143,634 + -3.1 143,748 + -1.1 143,748 + -1.1 143,749 - +1.6 143,780 - -0.9 143,896 - +1.4 144,141 - AO353_00680 0.65 +2.7 144,276 - -1.7 144,276 - +1.8 144,276 - -1.1 144,276 - -2.5 144,324 - +3.7 144,324 - +0.6 144,324 - -1.7 144,324 - -2.1 144,326 + +0.3 146,512 + +4.0 146,521 + -1.1 146,529 + +0.8 146,530 - +0.1 146,579 + +2.3 146,721 + AO353_00690 0.19 -2.7 146,721 + AO353_00690 0.19 +1.6 146,721 + AO353_00690 0.19 -1.1 146,836 - AO353_00690 0.34 -0.1 146,874 + AO353_00690 0.39 -0.1 146,874 + AO353_00690 0.39 +1.6 146,874 + AO353_00690 0.39 -0.1 146,875 - AO353_00690 0.39 -1.1 146,875 - AO353_00690 0.39 -0.1 146,984 - AO353_00690 0.53 -0.1 147,092 + AO353_00690 0.67 +0.9 147,100 - AO353_00690 0.69 -2.1 147,204 + AO353_00690 0.82 -2.1 147,204 + AO353_00690 0.82 +2.4 147,204 + AO353_00690 0.82 -0.9 147,204 + AO353_00690 0.82 -2.7 147,205 - AO353_00690 0.82 +2.2 147,205 - AO353_00690 0.82 -0.1 147,212 + AO353_00690 0.83 +1.8 147,301 - -2.5 147,301 - +1.4 147,337 - +0.2 147,337 - -1.1
Or see this region's nucleotide sequence