Experiment: MoYLS4 with Sisomicin 0.0156 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_3034 and DvMF_3035 are separated by 780 nucleotides DvMF_3035 and DvMF_R0078 are separated by 533 nucleotides DvMF_R0078 and DvMF_3036 are separated by 86 nucleotides
DvMF_3034: DvMF_3034 - 5,10-methylenetetrahydrofolate reductase (RefSeq), at 3,828,930 to 3,829,805
_3034
DvMF_3035: DvMF_3035 - glycosyl transferase family 9 (RefSeq), at 3,830,586 to 3,831,611
_3035
DvMF_R0078: DvMF_R0078 - tRNA-Lys (RefSeq), at 3,832,145 to 3,832,220
_R0078
DvMF_3036: DvMF_3036 - cytochrome c biogenesis protein transmembrane region (RefSeq), at 3,832,307 to 3,834,502
_3036
Position (kb)
3830
3831
3832 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3829.740 kb on + strand at 3829.878 kb on + strand at 3829.900 kb on + strand at 3830.122 kb on + strand at 3830.159 kb on + strand at 3830.319 kb on - strand at 3830.339 kb on - strand at 3830.480 kb on - strand at 3830.493 kb on + strand at 3830.530 kb on - strand at 3830.769 kb on + strand, within DvMF_3035 at 3830.927 kb on - strand, within DvMF_3035 at 3831.043 kb on - strand, within DvMF_3035 at 3831.227 kb on + strand, within DvMF_3035 at 3831.485 kb on - strand, within DvMF_3035 at 3831.583 kb on - strand at 3831.666 kb on - strand at 3831.760 kb on - strand at 3831.946 kb on + strand at 3832.371 kb on - strand at 3832.527 kb on - strand, within DvMF_3036
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Sisomicin 0.0156 mg/ml remove 3,829,740 + +0.2 3,829,878 + -0.6 3,829,900 + -0.3 3,830,122 + +0.2 3,830,159 + +0.4 3,830,319 - -0.2 3,830,339 - -0.6 3,830,480 - -0.9 3,830,493 + -0.3 3,830,530 - -0.1 3,830,769 + DvMF_3035 0.18 -1.8 3,830,927 - DvMF_3035 0.33 -2.1 3,831,043 - DvMF_3035 0.45 -4.3 3,831,227 + DvMF_3035 0.62 -1.2 3,831,485 - DvMF_3035 0.88 -4.3 3,831,583 - -1.1 3,831,666 - -0.5 3,831,760 - -0.0 3,831,946 + -0.4 3,832,371 - +0.0 3,832,527 - DvMF_3036 0.10 -0.5
Or see this region's nucleotide sequence