Experiment: MoYLS4 with Sisomicin 0.0156 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2171 and DvMF_2172 are separated by 148 nucleotides DvMF_2172 and DvMF_2173 are separated by 368 nucleotides DvMF_2173 and DvMF_2174 are separated by 24 nucleotides
DvMF_2171: DvMF_2171 - hypothetical protein (RefSeq), at 2,709,455 to 2,709,613
_2171
DvMF_2172: DvMF_2172 - DSBA oxidoreductase (RefSeq), at 2,709,762 to 2,710,565
_2172
DvMF_2173: DvMF_2173 - branched-chain amino acid transport (RefSeq), at 2,710,934 to 2,711,260
_2173
DvMF_2174: DvMF_2174 - AzlC family protein (RefSeq), at 2,711,285 to 2,712,133
_2174
Position (kb)
2709
2710
2711 Strain fitness (log2 ratio)
-2
-1
0
1 at 2708.846 kb on + strand at 2709.023 kb on + strand at 2709.090 kb on - strand at 2709.348 kb on + strand at 2709.452 kb on + strand at 2710.190 kb on + strand, within DvMF_2172 at 2710.235 kb on - strand, within DvMF_2172 at 2710.417 kb on + strand, within DvMF_2172 at 2710.524 kb on + strand at 2710.790 kb on - strand at 2710.886 kb on - strand at 2711.284 kb on + strand at 2711.290 kb on + strand at 2711.409 kb on + strand, within DvMF_2174 at 2711.417 kb on - strand, within DvMF_2174 at 2711.417 kb on - strand, within DvMF_2174 at 2711.417 kb on - strand, within DvMF_2174 at 2711.470 kb on + strand, within DvMF_2174
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Sisomicin 0.0156 mg/ml remove 2,708,846 + +0.0 2,709,023 + -0.7 2,709,090 - -0.6 2,709,348 + -0.4 2,709,452 + -0.7 2,710,190 + DvMF_2172 0.53 -1.8 2,710,235 - DvMF_2172 0.59 -1.6 2,710,417 + DvMF_2172 0.81 -2.2 2,710,524 + -1.3 2,710,790 - -0.8 2,710,886 - -0.7 2,711,284 + -0.3 2,711,290 + -0.3 2,711,409 + DvMF_2174 0.15 -0.6 2,711,417 - DvMF_2174 0.16 -0.1 2,711,417 - DvMF_2174 0.16 -0.2 2,711,417 - DvMF_2174 0.16 -0.4 2,711,470 + DvMF_2174 0.22 -0.5
Or see this region's nucleotide sequence