Experiment: MoYLS4 with Sisomicin 0.0156 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2103 and DvMF_2104 are separated by 23 nucleotides DvMF_2104 and DvMF_2105 are separated by 77 nucleotides DvMF_2105 and DvMF_2106 are separated by 90 nucleotides DvMF_2106 and DvMF_2107 are separated by 283 nucleotides
DvMF_2103: DvMF_2103 - protein of unknown function DUF81 (RefSeq), at 2,621,609 to 2,622,667
_2103
DvMF_2104: DvMF_2104 - hypothetical protein (RefSeq), at 2,622,691 to 2,623,011
_2104
DvMF_2105: DvMF_2105 - hypothetical protein (RefSeq), at 2,623,089 to 2,623,646
_2105
DvMF_2106: DvMF_2106 - hypothetical protein (RefSeq), at 2,623,737 to 2,623,976
_2106
DvMF_2107: DvMF_2107 - response regulator receiver protein (RefSeq), at 2,624,260 to 2,626,566
_2107
Position (kb)
2623
2624 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2623.061 kb on - strand at 2623.106 kb on + strand at 2623.142 kb on + strand at 2623.170 kb on - strand, within DvMF_2105 at 2623.453 kb on + strand, within DvMF_2105 at 2623.707 kb on - strand at 2623.824 kb on + strand, within DvMF_2106 at 2623.912 kb on - strand, within DvMF_2106 at 2624.432 kb on + strand at 2624.432 kb on + strand at 2624.435 kb on - strand at 2624.442 kb on + strand at 2624.555 kb on + strand, within DvMF_2107 at 2624.562 kb on - strand, within DvMF_2107 at 2624.603 kb on + strand, within DvMF_2107 at 2624.624 kb on - strand, within DvMF_2107
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Sisomicin 0.0156 mg/ml remove 2,623,061 - +0.6 2,623,106 + -2.1 2,623,142 + -3.7 2,623,170 - DvMF_2105 0.15 -0.9 2,623,453 + DvMF_2105 0.65 -1.9 2,623,707 - -1.0 2,623,824 + DvMF_2106 0.36 -1.1 2,623,912 - DvMF_2106 0.73 -0.3 2,624,432 + +0.4 2,624,432 + -0.2 2,624,435 - -0.9 2,624,442 + -1.0 2,624,555 + DvMF_2107 0.13 -0.2 2,624,562 - DvMF_2107 0.13 -0.5 2,624,603 + DvMF_2107 0.15 +0.1 2,624,624 - DvMF_2107 0.16 -0.7
Or see this region's nucleotide sequence