Experiment: MoYLS4 with Sisomicin 0.0156 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2053 and DvMF_2054 are separated by 348 nucleotides DvMF_2054 and DvMF_2055 are separated by 4 nucleotides DvMF_2055 and DvMF_2056 overlap by 1 nucleotides DvMF_2056 and DvMF_2057 overlap by 1 nucleotides
DvMF_2053: DvMF_2053 - S-adenosylmethionine/tRNA-ribosyltransferase- isomerase (RefSeq), at 2,545,433 to 2,546,686
_2053
DvMF_2054: DvMF_2054 - 4Fe-4S ferredoxin iron-sulfur binding domain protein (RefSeq), at 2,547,035 to 2,547,274
_2054
DvMF_2055: DvMF_2055 - 2-ketoisovalerate ferredoxin reductase (RefSeq), at 2,547,279 to 2,548,364
_2055
DvMF_2056: DvMF_2056 - thiamine pyrophosphate protein domain protein TPP-binding (RefSeq), at 2,548,364 to 2,549,155
_2056
DvMF_2057: DvMF_2057 - pyruvate ferredoxin/flavodoxin oxidoreductase (RefSeq), at 2,549,155 to 2,549,697
_2057
Position (kb)
2547
2548
2549 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2546.287 kb on - strand, within DvMF_2053 at 2546.351 kb on - strand, within DvMF_2053 at 2546.450 kb on + strand, within DvMF_2053 at 2546.643 kb on + strand at 2546.794 kb on - strand at 2546.826 kb on + strand at 2547.084 kb on + strand, within DvMF_2054 at 2547.111 kb on - strand, within DvMF_2054 at 2547.178 kb on + strand, within DvMF_2054 at 2547.181 kb on - strand, within DvMF_2054 at 2547.189 kb on - strand, within DvMF_2054 at 2547.269 kb on + strand at 2547.269 kb on - strand at 2547.274 kb on + strand at 2547.425 kb on + strand, within DvMF_2055 at 2548.007 kb on + strand, within DvMF_2055 at 2548.223 kb on + strand, within DvMF_2055 at 2548.356 kb on - strand at 2548.430 kb on + strand at 2548.518 kb on + strand, within DvMF_2056 at 2548.987 kb on - strand, within DvMF_2056 at 2549.221 kb on + strand, within DvMF_2057
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Sisomicin 0.0156 mg/ml remove 2,546,287 - DvMF_2053 0.68 -1.1 2,546,351 - DvMF_2053 0.73 +0.1 2,546,450 + DvMF_2053 0.81 -1.4 2,546,643 + -1.9 2,546,794 - -2.9 2,546,826 + -1.5 2,547,084 + DvMF_2054 0.20 -1.9 2,547,111 - DvMF_2054 0.32 -2.7 2,547,178 + DvMF_2054 0.60 -3.0 2,547,181 - DvMF_2054 0.61 -2.1 2,547,189 - DvMF_2054 0.64 -0.1 2,547,269 + -0.1 2,547,269 - -3.2 2,547,274 + -2.1 2,547,425 + DvMF_2055 0.13 -0.9 2,548,007 + DvMF_2055 0.67 -2.2 2,548,223 + DvMF_2055 0.87 -2.4 2,548,356 - -1.3 2,548,430 + -3.9 2,548,518 + DvMF_2056 0.19 -2.2 2,548,987 - DvMF_2056 0.79 -1.7 2,549,221 + DvMF_2057 0.12 -2.4
Or see this region's nucleotide sequence