Experiment: MoYLS4 with Sisomicin 0.0156 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2035 and DvMF_2036 are separated by 362 nucleotides DvMF_2036 and hisS are separated by 309 nucleotides
DvMF_2035: DvMF_2035 - 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase (RefSeq), at 2,522,834 to 2,525,176
_2035
DvMF_2036: DvMF_2036 - Methyltransferase type 11 (RefSeq), at 2,525,539 to 2,526,345
_2036
DvMF_2037: hisS - histidyl-tRNA synthetase (RefSeq), at 2,526,655 to 2,527,935
hisS
Position (kb)
2525
2526
2527 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2524.567 kb on - strand, within DvMF_2035 at 2524.618 kb on - strand, within DvMF_2035 at 2524.710 kb on + strand, within DvMF_2035 at 2524.728 kb on - strand, within DvMF_2035 at 2524.735 kb on - strand, within DvMF_2035 at 2524.884 kb on + strand, within DvMF_2035 at 2524.907 kb on - strand, within DvMF_2035 at 2525.008 kb on - strand at 2525.117 kb on + strand at 2525.296 kb on + strand at 2525.306 kb on - strand at 2525.463 kb on + strand at 2525.463 kb on + strand at 2525.502 kb on - strand at 2525.573 kb on + strand at 2525.647 kb on + strand, within DvMF_2036 at 2525.664 kb on + strand, within DvMF_2036 at 2525.751 kb on + strand, within DvMF_2036 at 2525.754 kb on - strand, within DvMF_2036 at 2525.869 kb on + strand, within DvMF_2036 at 2525.874 kb on - strand, within DvMF_2036 at 2526.077 kb on + strand, within DvMF_2036 at 2526.087 kb on + strand, within DvMF_2036 at 2526.112 kb on + strand, within DvMF_2036 at 2526.187 kb on - strand, within DvMF_2036 at 2526.322 kb on - strand at 2526.511 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Sisomicin 0.0156 mg/ml remove 2,524,567 - DvMF_2035 0.74 -1.3 2,524,618 - DvMF_2035 0.76 -0.1 2,524,710 + DvMF_2035 0.80 -1.4 2,524,728 - DvMF_2035 0.81 +0.5 2,524,735 - DvMF_2035 0.81 -0.5 2,524,884 + DvMF_2035 0.87 -1.3 2,524,907 - DvMF_2035 0.88 -0.9 2,525,008 - -0.3 2,525,117 + -1.0 2,525,296 + +0.1 2,525,306 - -0.3 2,525,463 + -2.1 2,525,463 + +0.2 2,525,502 - -0.9 2,525,573 + -1.7 2,525,647 + DvMF_2036 0.13 -1.5 2,525,664 + DvMF_2036 0.15 -2.0 2,525,751 + DvMF_2036 0.26 -1.9 2,525,754 - DvMF_2036 0.27 -2.9 2,525,869 + DvMF_2036 0.41 -1.2 2,525,874 - DvMF_2036 0.42 -0.9 2,526,077 + DvMF_2036 0.67 -2.5 2,526,087 + DvMF_2036 0.68 -2.0 2,526,112 + DvMF_2036 0.71 -2.9 2,526,187 - DvMF_2036 0.80 -3.3 2,526,322 - -0.4 2,526,511 + -0.8
Or see this region's nucleotide sequence