Experiment: MoYLS4 with Sisomicin 0.0156 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1909 and DvMF_1910 are separated by 25 nucleotides DvMF_1910 and DvMF_1911 are separated by 174 nucleotides DvMF_1911 and DvMF_1912 are separated by 157 nucleotides
DvMF_1909: DvMF_1909 - CDP-glucose 4,6-dehydratase (RefSeq), at 2,353,476 to 2,354,585
_1909
DvMF_1910: DvMF_1910 - polysaccharide biosynthesis domain-containing protein (RefSeq), at 2,354,611 to 2,355,060
_1910
DvMF_1911: DvMF_1911 - DegT/DnrJ/EryC1/StrS aminotransferase (RefSeq), at 2,355,235 to 2,356,368
_1911
DvMF_1912: DvMF_1912 - glycosyl transferase family 2 (RefSeq), at 2,356,526 to 2,357,554
_1912
Position (kb)
2355
2356
2357 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2354.299 kb on + strand, within DvMF_1909 at 2354.616 kb on - strand at 2355.096 kb on + strand at 2355.374 kb on + strand, within DvMF_1911 at 2355.661 kb on + strand, within DvMF_1911 at 2355.661 kb on + strand, within DvMF_1911 at 2356.086 kb on + strand, within DvMF_1911 at 2356.091 kb on + strand, within DvMF_1911 at 2356.187 kb on - strand, within DvMF_1911 at 2356.703 kb on - strand, within DvMF_1912 at 2356.916 kb on + strand, within DvMF_1912 at 2356.999 kb on + strand, within DvMF_1912 at 2357.337 kb on - strand, within DvMF_1912
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Sisomicin 0.0156 mg/ml remove 2,354,299 + DvMF_1909 0.74 -3.1 2,354,616 - +0.2 2,355,096 + -0.8 2,355,374 + DvMF_1911 0.12 -3.5 2,355,661 + DvMF_1911 0.38 -3.9 2,355,661 + DvMF_1911 0.38 -2.0 2,356,086 + DvMF_1911 0.75 -3.6 2,356,091 + DvMF_1911 0.75 -4.2 2,356,187 - DvMF_1911 0.84 -3.1 2,356,703 - DvMF_1912 0.17 -3.3 2,356,916 + DvMF_1912 0.38 -4.0 2,356,999 + DvMF_1912 0.46 -3.2 2,357,337 - DvMF_1912 0.79 -1.9
Or see this region's nucleotide sequence