Experiment: MoYLS4 with Sisomicin 0.0156 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1900 and DvMF_1901 are separated by 10 nucleotides DvMF_1901 and DvMF_1902 are separated by 129 nucleotides DvMF_1902 and DvMF_1903 are separated by 144 nucleotides
DvMF_1900: DvMF_1900 - 3-deoxy-manno-octulosonate cytidylyltransferase (RefSeq), at 2,339,805 to 2,340,575
_1900
DvMF_1901: DvMF_1901 - transferase hexapeptide repeat containing protein (RefSeq), at 2,340,586 to 2,341,224
_1901
DvMF_1902: DvMF_1902 - D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding (RefSeq), at 2,341,354 to 2,342,259
_1902
DvMF_1903: DvMF_1903 - Haloacid dehalogenase domain protein hydrolase (RefSeq), at 2,342,404 to 2,343,045
_1903
Position (kb)
2341
2342
2343 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2340.774 kb on - strand, within DvMF_1901 at 2340.912 kb on - strand, within DvMF_1901 at 2341.176 kb on + strand at 2341.238 kb on - strand at 2341.568 kb on - strand, within DvMF_1902 at 2341.661 kb on + strand, within DvMF_1902 at 2341.756 kb on - strand, within DvMF_1902 at 2342.002 kb on - strand, within DvMF_1902 at 2342.002 kb on - strand, within DvMF_1902 at 2342.598 kb on - strand, within DvMF_1903 at 2342.845 kb on - strand, within DvMF_1903 at 2343.032 kb on - strand at 2343.145 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Sisomicin 0.0156 mg/ml remove 2,340,774 - DvMF_1901 0.29 -0.9 2,340,912 - DvMF_1901 0.51 +1.5 2,341,176 + +0.0 2,341,238 - -0.3 2,341,568 - DvMF_1902 0.24 -1.7 2,341,661 + DvMF_1902 0.34 -0.8 2,341,756 - DvMF_1902 0.44 -4.0 2,342,002 - DvMF_1902 0.72 -0.2 2,342,002 - DvMF_1902 0.72 -2.1 2,342,598 - DvMF_1903 0.30 -2.8 2,342,845 - DvMF_1903 0.69 -2.5 2,343,032 - -0.1 2,343,145 + -0.2
Or see this region's nucleotide sequence