Experiment: MoYLS4 with Sisomicin 0.0156 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1424 and pyrB are separated by 14 nucleotides pyrB and DvMF_1426 overlap by 4 nucleotides
DvMF_1424: DvMF_1424 - amidohydrolase (RefSeq), at 1,741,176 to 1,742,612
_1424
DvMF_1425: pyrB - aspartate carbamoyltransferase catalytic subunit (RefSeq), at 1,742,627 to 1,743,604
pyrB
DvMF_1426: DvMF_1426 - dihydroorotase, multifunctional complex type (RefSeq), at 1,743,601 to 1,744,881
_1426
Position (kb)
1743
1744
1745 Strain fitness (log2 ratio)
-2
-1
0
1 at 1742.691 kb on + strand at 1742.777 kb on - strand, within pyrB at 1742.886 kb on + strand, within pyrB at 1742.904 kb on - strand, within pyrB at 1743.023 kb on - strand, within pyrB at 1743.083 kb on - strand, within pyrB at 1743.091 kb on + strand, within pyrB at 1743.151 kb on - strand, within pyrB at 1743.176 kb on - strand, within pyrB at 1743.200 kb on - strand, within pyrB at 1743.268 kb on - strand, within pyrB at 1743.441 kb on - strand, within pyrB at 1743.492 kb on + strand, within pyrB at 1743.542 kb on - strand at 1743.575 kb on - strand at 1743.660 kb on + strand at 1743.808 kb on + strand, within DvMF_1426 at 1743.816 kb on + strand, within DvMF_1426 at 1743.864 kb on + strand, within DvMF_1426 at 1743.900 kb on + strand, within DvMF_1426 at 1743.931 kb on - strand, within DvMF_1426 at 1743.941 kb on - strand, within DvMF_1426 at 1744.178 kb on - strand, within DvMF_1426 at 1744.178 kb on - strand, within DvMF_1426 at 1744.330 kb on + strand, within DvMF_1426 at 1744.759 kb on + strand at 1744.883 kb on - strand at 1744.955 kb on + strand at 1744.963 kb on - strand at 1744.973 kb on - strand at 1744.973 kb on - strand at 1745.149 kb on + strand at 1745.207 kb on - strand at 1745.252 kb on + strand at 1745.321 kb on - strand at 1745.680 kb on + strand at 1745.850 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Sisomicin 0.0156 mg/ml remove 1,742,691 + -2.0 1,742,777 - pyrB DvMF_1425 0.15 -1.0 1,742,886 + pyrB DvMF_1425 0.26 -1.0 1,742,904 - pyrB DvMF_1425 0.28 -2.0 1,743,023 - pyrB DvMF_1425 0.40 -1.3 1,743,083 - pyrB DvMF_1425 0.47 -1.1 1,743,091 + pyrB DvMF_1425 0.47 -0.7 1,743,151 - pyrB DvMF_1425 0.54 -1.3 1,743,176 - pyrB DvMF_1425 0.56 -1.3 1,743,200 - pyrB DvMF_1425 0.59 -0.9 1,743,268 - pyrB DvMF_1425 0.66 -2.1 1,743,441 - pyrB DvMF_1425 0.83 -1.9 1,743,492 + pyrB DvMF_1425 0.88 -0.8 1,743,542 - -0.9 1,743,575 - -0.3 1,743,660 + -1.5 1,743,808 + DvMF_1426 0.16 -1.3 1,743,816 + DvMF_1426 0.17 -1.3 1,743,864 + DvMF_1426 0.21 -1.3 1,743,900 + DvMF_1426 0.23 -2.1 1,743,931 - DvMF_1426 0.26 -2.1 1,743,941 - DvMF_1426 0.27 -1.1 1,744,178 - DvMF_1426 0.45 -2.1 1,744,178 - DvMF_1426 0.45 -0.7 1,744,330 + DvMF_1426 0.57 -0.9 1,744,759 + -0.9 1,744,883 - -0.5 1,744,955 + -0.0 1,744,963 - +0.1 1,744,973 - -0.5 1,744,973 - -0.2 1,745,149 + +0.1 1,745,207 - -0.4 1,745,252 + -0.3 1,745,321 - -0.3 1,745,680 + -0.6 1,745,850 + +0.8
Or see this region's nucleotide sequence