Experiment: MoYLS4 with Sisomicin 0.0156 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1172 and DvMF_1173 are separated by 148 nucleotides DvMF_1173 and DvMF_1174 are separated by 340 nucleotides DvMF_1174 and DvMF_1175 overlap by 4 nucleotides
DvMF_1172: DvMF_1172 - Rhodanese domain protein (RefSeq), at 1,449,223 to 1,449,744
_1172
DvMF_1173: DvMF_1173 - hypothetical protein (RefSeq), at 1,449,893 to 1,450,369
_1173
DvMF_1174: DvMF_1174 - cobalamin (vitamin B12) biosynthesis CbiD protein (RefSeq), at 1,450,710 to 1,452,059
_1174
DvMF_1175: DvMF_1175 - precorrin-6y C5,15-methyltransferase (decarboxylating), CbiE subunit (RefSeq), at 1,452,056 to 1,453,480
_1175
Position (kb)
1450
1451
1452
1453 Strain fitness (log2 ratio)
-1
0
1 at 1449.952 kb on + strand, within DvMF_1173 at 1449.960 kb on - strand, within DvMF_1173 at 1450.004 kb on + strand, within DvMF_1173 at 1450.200 kb on - strand, within DvMF_1173 at 1450.872 kb on - strand, within DvMF_1174 at 1451.043 kb on - strand, within DvMF_1174 at 1451.337 kb on - strand, within DvMF_1174 at 1451.614 kb on + strand, within DvMF_1174 at 1451.646 kb on - strand, within DvMF_1174 at 1451.717 kb on - strand, within DvMF_1174 at 1451.907 kb on - strand, within DvMF_1174 at 1453.053 kb on + strand, within DvMF_1175
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Sisomicin 0.0156 mg/ml remove 1,449,952 + DvMF_1173 0.12 -0.4 1,449,960 - DvMF_1173 0.14 -0.5 1,450,004 + DvMF_1173 0.23 -0.4 1,450,200 - DvMF_1173 0.64 -0.2 1,450,872 - DvMF_1174 0.12 -0.9 1,451,043 - DvMF_1174 0.25 -1.5 1,451,337 - DvMF_1174 0.46 -0.3 1,451,614 + DvMF_1174 0.67 -1.3 1,451,646 - DvMF_1174 0.69 -1.6 1,451,717 - DvMF_1174 0.75 -1.4 1,451,907 - DvMF_1174 0.89 -0.7 1,453,053 + DvMF_1175 0.70 -0.7
Or see this region's nucleotide sequence