Experiment: MoYLS4 with Sisomicin 0.0156 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0333 and DvMF_R0016 are separated by 252 nucleotides DvMF_R0016 and DvMF_0334 are separated by 313 nucleotides DvMF_0334 and DvMF_0335 are separated by 339 nucleotides DvMF_0335 and DvMF_0336 are separated by 165 nucleotides
DvMF_0333: DvMF_0333 - ADP-ribosylation/Crystallin J1 (RefSeq), at 388,796 to 389,899
_0333
DvMF_R0016: DvMF_R0016 - tRNA-Arg (RefSeq), at 390,152 to 390,228
_R0016
DvMF_0334: DvMF_0334 - Glutamate--tRNA ligase (RefSeq), at 390,542 to 391,663
_0334
DvMF_0335: DvMF_0335 - hypothetical protein (RefSeq), at 392,003 to 392,395
_0335
DvMF_0336: DvMF_0336 - transcriptional regulator, TetR family (RefSeq), at 392,561 to 393,136
_0336
Position (kb) 390
391
392 Strain fitness (log2 ratio) -2
-1
0
1 at 389.595 kb on - strand, within DvMF_0333 at 389.701 kb on + strand, within DvMF_0333 at 389.751 kb on + strand, within DvMF_0333 at 389.796 kb on + strand at 389.804 kb on - strand at 389.823 kb on - strand at 389.892 kb on - strand at 390.034 kb on - strand at 390.053 kb on - strand at 390.735 kb on + strand, within DvMF_0334 at 390.793 kb on + strand, within DvMF_0334 at 390.825 kb on - strand, within DvMF_0334 at 390.971 kb on + strand, within DvMF_0334 at 391.006 kb on - strand, within DvMF_0334 at 391.006 kb on - strand, within DvMF_0334 at 391.414 kb on - strand, within DvMF_0334 at 391.565 kb on - strand at 391.728 kb on + strand at 391.845 kb on + strand at 391.880 kb on - strand at 392.105 kb on + strand, within DvMF_0335 at 392.261 kb on + strand, within DvMF_0335 at 392.294 kb on + strand, within DvMF_0335
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Sisomicin 0.0156 mg/ml remove 389,595 - DvMF_0333 0.72 -0.5 389,701 + DvMF_0333 0.82 -0.5 389,751 + DvMF_0333 0.87 -0.8 389,796 + -0.7 389,804 - -0.3 389,823 - -1.9 389,892 - -0.5 390,034 - -0.3 390,053 - -0.5 390,735 + DvMF_0334 0.17 -1.4 390,793 + DvMF_0334 0.22 -0.6 390,825 - DvMF_0334 0.25 -1.3 390,971 + DvMF_0334 0.38 -0.7 391,006 - DvMF_0334 0.41 -1.2 391,006 - DvMF_0334 0.41 -0.0 391,414 - DvMF_0334 0.78 -1.9 391,565 - -1.0 391,728 + -1.0 391,845 + -0.3 391,880 - -0.9 392,105 + DvMF_0335 0.26 -0.3 392,261 + DvMF_0335 0.66 -0.4 392,294 + DvMF_0335 0.74 -0.2
Or see this region's nucleotide sequence