Experiment: MoYLS4 with Sisomicin 0.0156 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0200 and DvMF_0201 are separated by 189 nucleotides DvMF_0201 and DvMF_0202 are separated by 88 nucleotides DvMF_0202 and DvMF_0203 are separated by 237 nucleotides
DvMF_0200: DvMF_0200 - Radical SAM domain protein (RefSeq), at 217,942 to 219,102
_0200
DvMF_0201: DvMF_0201 - hypothetical protein (RefSeq), at 219,292 to 219,768
_0201
DvMF_0202: DvMF_0202 - phosphoribosylaminoimidazole synthetase (RefSeq), at 219,857 to 220,909
_0202
DvMF_0203: DvMF_0203 - Radical SAM domain protein (RefSeq), at 221,147 to 222,682
_0203
Position (kb)
219
220
221 Strain fitness (log2 ratio)
-2
-1
0
1 at 218.860 kb on - strand, within DvMF_0200 at 218.995 kb on - strand at 219.184 kb on + strand at 219.325 kb on + strand at 219.333 kb on - strand at 219.886 kb on - strand at 219.958 kb on + strand at 220.270 kb on + strand, within DvMF_0202 at 220.816 kb on - strand at 220.979 kb on + strand at 220.992 kb on - strand at 220.992 kb on - strand at 220.992 kb on - strand at 221.002 kb on - strand at 221.110 kb on + strand at 221.118 kb on - strand at 221.230 kb on - strand at 221.322 kb on - strand, within DvMF_0203 at 221.796 kb on - strand, within DvMF_0203
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Sisomicin 0.0156 mg/ml remove 218,860 - DvMF_0200 0.79 +0.3 218,995 - -1.1 219,184 + -1.2 219,325 + -0.4 219,333 - -0.3 219,886 - -0.9 219,958 + -2.4 220,270 + DvMF_0202 0.39 -1.2 220,816 - -0.8 220,979 + -0.8 220,992 - +0.9 220,992 - -1.2 220,992 - -0.6 221,002 - -0.2 221,110 + -0.1 221,118 - -0.2 221,230 - -0.0 221,322 - DvMF_0203 0.11 -0.7 221,796 - DvMF_0203 0.42 +1.7
Or see this region's nucleotide sequence