Experiment: MoYLS4 with Sisomicin 0.0156 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt clpX and DvMF_0112 are separated by 118 nucleotides DvMF_0112 and DvMF_0113 are separated by 397 nucleotides DvMF_0113 and DvMF_0114 are separated by 22 nucleotides
DvMF_0111: clpX - ATP-dependent protease ATP-binding subunit ClpX (RefSeq), at 120,238 to 121,776
clpX
DvMF_0112: DvMF_0112 - ATP-dependent protease La (RefSeq), at 121,895 to 124,357
_0112
DvMF_0113: DvMF_0113 - hypothetical protein (RefSeq), at 124,755 to 125,330
_0113
DvMF_0114: DvMF_0114 - hypothetical protein (RefSeq), at 125,353 to 125,619
_0114
Position (kb) 121
122
123
124
125 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 121.199 kb on + strand, within clpX at 121.982 kb on + strand at 122.203 kb on - strand, within DvMF_0112 at 122.307 kb on - strand, within DvMF_0112 at 122.336 kb on + strand, within DvMF_0112 at 122.620 kb on + strand, within DvMF_0112 at 122.628 kb on - strand, within DvMF_0112 at 122.687 kb on - strand, within DvMF_0112 at 123.265 kb on + strand, within DvMF_0112 at 123.273 kb on - strand, within DvMF_0112 at 123.375 kb on + strand, within DvMF_0112 at 123.831 kb on - strand, within DvMF_0112 at 124.375 kb on - strand at 124.789 kb on + strand at 124.854 kb on + strand, within DvMF_0113 at 124.869 kb on - strand, within DvMF_0113 at 125.031 kb on + strand, within DvMF_0113 at 125.032 kb on + strand, within DvMF_0113 at 125.035 kb on - strand, within DvMF_0113 at 125.209 kb on - strand, within DvMF_0113 at 125.270 kb on - strand, within DvMF_0113
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Sisomicin 0.0156 mg/ml remove 121,199 + clpX DvMF_0111 0.62 +0.4 121,982 + -0.3 122,203 - DvMF_0112 0.13 -1.0 122,307 - DvMF_0112 0.17 -1.2 122,336 + DvMF_0112 0.18 -0.4 122,620 + DvMF_0112 0.29 -1.3 122,628 - DvMF_0112 0.30 -2.8 122,687 - DvMF_0112 0.32 -1.0 123,265 + DvMF_0112 0.56 +0.4 123,273 - DvMF_0112 0.56 -0.6 123,375 + DvMF_0112 0.60 -1.1 123,831 - DvMF_0112 0.79 -0.2 124,375 - +0.2 124,789 + -0.7 124,854 + DvMF_0113 0.17 -0.6 124,869 - DvMF_0113 0.20 +0.1 125,031 + DvMF_0113 0.48 -0.4 125,032 + DvMF_0113 0.48 -0.0 125,035 - DvMF_0113 0.49 -0.1 125,209 - DvMF_0113 0.79 +0.1 125,270 - DvMF_0113 0.89 -0.4
Or see this region's nucleotide sequence