Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3806 and Pf6N2E2_3807 overlap by 4 nucleotides Pf6N2E2_3807 and Pf6N2E2_3808 are separated by 104 nucleotides
Pf6N2E2_3806 - Conserved hypothetical protein (perhaps related to histidine degradation), at 4,435,983 to 4,436,552
_3806
Pf6N2E2_3807 - Histidine utilization repressor, at 4,436,549 to 4,437,298
_3807
Pf6N2E2_3808 - Formiminoglutamic iminohydrolase (EC 3.5.3.13) (from data) , at 4,437,403 to 4,438,767
_3808
Position (kb)
4436
4437
4438 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4435.587 kb on - strand at 4435.587 kb on - strand at 4435.902 kb on + strand at 4435.902 kb on + strand at 4435.902 kb on + strand at 4435.902 kb on + strand at 4435.902 kb on + strand at 4436.071 kb on + strand, within Pf6N2E2_3806 at 4436.072 kb on - strand, within Pf6N2E2_3806 at 4436.215 kb on + strand, within Pf6N2E2_3806 at 4436.215 kb on + strand, within Pf6N2E2_3806 at 4436.216 kb on - strand, within Pf6N2E2_3806 at 4436.216 kb on - strand, within Pf6N2E2_3806 at 4436.519 kb on - strand at 4436.555 kb on - strand at 4436.555 kb on - strand at 4436.555 kb on - strand at 4436.555 kb on - strand at 4436.635 kb on + strand, within Pf6N2E2_3807 at 4436.652 kb on - strand, within Pf6N2E2_3807 at 4436.760 kb on + strand, within Pf6N2E2_3807 at 4436.761 kb on - strand, within Pf6N2E2_3807 at 4436.767 kb on - strand, within Pf6N2E2_3807 at 4436.799 kb on + strand, within Pf6N2E2_3807 at 4436.799 kb on + strand, within Pf6N2E2_3807 at 4437.007 kb on + strand, within Pf6N2E2_3807 at 4437.007 kb on + strand, within Pf6N2E2_3807 at 4437.008 kb on - strand, within Pf6N2E2_3807 at 4437.008 kb on - strand, within Pf6N2E2_3807 at 4437.235 kb on - strand at 4437.329 kb on + strand at 4437.330 kb on - strand at 4437.330 kb on - strand at 4437.330 kb on - strand at 4437.330 kb on - strand at 4437.330 kb on - strand at 4437.365 kb on + strand at 4437.365 kb on + strand at 4437.367 kb on + strand at 4437.367 kb on + strand at 4437.368 kb on - strand at 4437.371 kb on + strand at 4437.371 kb on + strand at 4437.371 kb on + strand at 4437.371 kb on + strand at 4437.371 kb on + strand at 4437.372 kb on - strand at 4437.372 kb on - strand at 4437.372 kb on - strand at 4437.374 kb on - strand at 4437.374 kb on - strand at 4437.458 kb on + strand at 4437.458 kb on + strand at 4437.458 kb on + strand at 4437.458 kb on + strand at 4437.458 kb on + strand at 4437.459 kb on - strand at 4437.459 kb on - strand at 4437.658 kb on + strand, within Pf6N2E2_3808 at 4437.659 kb on - strand, within Pf6N2E2_3808 at 4437.715 kb on + strand, within Pf6N2E2_3808 at 4437.716 kb on - strand, within Pf6N2E2_3808 at 4437.716 kb on - strand, within Pf6N2E2_3808 at 4437.739 kb on + strand, within Pf6N2E2_3808 at 4437.763 kb on + strand, within Pf6N2E2_3808 at 4437.763 kb on + strand, within Pf6N2E2_3808 at 4437.763 kb on + strand, within Pf6N2E2_3808 at 4437.763 kb on + strand, within Pf6N2E2_3808 at 4437.764 kb on - strand, within Pf6N2E2_3808 at 4437.764 kb on - strand, within Pf6N2E2_3808 at 4437.764 kb on - strand, within Pf6N2E2_3808 at 4437.794 kb on - strand, within Pf6N2E2_3808 at 4437.877 kb on + strand, within Pf6N2E2_3808 at 4437.877 kb on + strand, within Pf6N2E2_3808 at 4437.878 kb on - strand, within Pf6N2E2_3808 at 4437.878 kb on - strand, within Pf6N2E2_3808 at 4437.949 kb on + strand, within Pf6N2E2_3808 at 4437.950 kb on - strand, within Pf6N2E2_3808 at 4438.169 kb on + strand, within Pf6N2E2_3808 at 4438.169 kb on + strand, within Pf6N2E2_3808
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 4,435,587 - +0.1 4,435,587 - +1.0 4,435,902 + -0.4 4,435,902 + +0.0 4,435,902 + +0.6 4,435,902 + +0.5 4,435,902 + -1.2 4,436,071 + Pf6N2E2_3806 0.15 +0.7 4,436,072 - Pf6N2E2_3806 0.16 +0.9 4,436,215 + Pf6N2E2_3806 0.41 -0.1 4,436,215 + Pf6N2E2_3806 0.41 +0.0 4,436,216 - Pf6N2E2_3806 0.41 -0.3 4,436,216 - Pf6N2E2_3806 0.41 +0.2 4,436,519 - -0.3 4,436,555 - +0.7 4,436,555 - -0.4 4,436,555 - +0.3 4,436,555 - -0.4 4,436,635 + Pf6N2E2_3807 0.11 +0.0 4,436,652 - Pf6N2E2_3807 0.14 -0.5 4,436,760 + Pf6N2E2_3807 0.28 -0.2 4,436,761 - Pf6N2E2_3807 0.28 +1.0 4,436,767 - Pf6N2E2_3807 0.29 -0.2 4,436,799 + Pf6N2E2_3807 0.33 -0.1 4,436,799 + Pf6N2E2_3807 0.33 -1.8 4,437,007 + Pf6N2E2_3807 0.61 -2.5 4,437,007 + Pf6N2E2_3807 0.61 -1.9 4,437,008 - Pf6N2E2_3807 0.61 -0.3 4,437,008 - Pf6N2E2_3807 0.61 -0.6 4,437,235 - +0.2 4,437,329 + -0.9 4,437,330 - -0.4 4,437,330 - +0.4 4,437,330 - +0.5 4,437,330 - +1.0 4,437,330 - -0.9 4,437,365 + +0.3 4,437,365 + +0.1 4,437,367 + -0.6 4,437,367 + +0.6 4,437,368 - +0.8 4,437,371 + +0.0 4,437,371 + +1.2 4,437,371 + -1.5 4,437,371 + +0.4 4,437,371 + +0.3 4,437,372 - +1.0 4,437,372 - +0.6 4,437,372 - +0.2 4,437,374 - -0.1 4,437,374 - +0.7 4,437,458 + +0.8 4,437,458 + +0.9 4,437,458 + +0.1 4,437,458 + +1.9 4,437,458 + -0.3 4,437,459 - -0.8 4,437,459 - -0.9 4,437,658 + Pf6N2E2_3808 0.19 +1.4 4,437,659 - Pf6N2E2_3808 0.19 +0.9 4,437,715 + Pf6N2E2_3808 0.23 +0.2 4,437,716 - Pf6N2E2_3808 0.23 -1.4 4,437,716 - Pf6N2E2_3808 0.23 -0.2 4,437,739 + Pf6N2E2_3808 0.25 +0.8 4,437,763 + Pf6N2E2_3808 0.26 +0.7 4,437,763 + Pf6N2E2_3808 0.26 -0.3 4,437,763 + Pf6N2E2_3808 0.26 +0.7 4,437,763 + Pf6N2E2_3808 0.26 +0.1 4,437,764 - Pf6N2E2_3808 0.26 -0.6 4,437,764 - Pf6N2E2_3808 0.26 -0.1 4,437,764 - Pf6N2E2_3808 0.26 +1.0 4,437,794 - Pf6N2E2_3808 0.29 +0.8 4,437,877 + Pf6N2E2_3808 0.35 -0.6 4,437,877 + Pf6N2E2_3808 0.35 +2.0 4,437,878 - Pf6N2E2_3808 0.35 +1.3 4,437,878 - Pf6N2E2_3808 0.35 +1.0 4,437,949 + Pf6N2E2_3808 0.40 +0.0 4,437,950 - Pf6N2E2_3808 0.40 -0.1 4,438,169 + Pf6N2E2_3808 0.56 +0.6 4,438,169 + Pf6N2E2_3808 0.56 +0.5
Or see this region's nucleotide sequence