Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3383 and Pf6N2E2_3384 overlap by 4 nucleotides Pf6N2E2_3384 and Pf6N2E2_3385 overlap by 4 nucleotides Pf6N2E2_3385 and Pf6N2E2_3386 are separated by 127 nucleotides
Pf6N2E2_3383 - Predicted L-lactate dehydrogenase, Iron-sulfur cluster-binding subunit YkgF, at 3,913,761 to 3,914,489
_3383
Pf6N2E2_3384 - Predicted L-lactate dehydrogenase, hypothetical protein subunit YkgG, at 3,914,486 to 3,915,157
_3384
Pf6N2E2_3385 - Predicted D-lactate dehydrogenase, Fe-S protein, FAD/FMN-containing, at 3,915,154 to 3,917,964
_3385
Pf6N2E2_3386 - hypothetical protein, at 3,918,092 to 3,918,370
_3386
Position (kb)
3915
3916
3917
3918 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3914.271 kb on + strand, within Pf6N2E2_3383 at 3914.272 kb on - strand, within Pf6N2E2_3383 at 3914.572 kb on - strand, within Pf6N2E2_3384 at 3914.572 kb on - strand, within Pf6N2E2_3384 at 3914.585 kb on + strand, within Pf6N2E2_3384 at 3915.002 kb on + strand, within Pf6N2E2_3384 at 3915.002 kb on + strand, within Pf6N2E2_3384 at 3915.002 kb on + strand, within Pf6N2E2_3384 at 3915.003 kb on - strand, within Pf6N2E2_3384 at 3915.003 kb on - strand, within Pf6N2E2_3384 at 3915.003 kb on - strand, within Pf6N2E2_3384 at 3915.176 kb on + strand at 3915.176 kb on + strand at 3915.176 kb on + strand at 3915.176 kb on + strand at 3915.177 kb on - strand at 3915.288 kb on - strand at 3915.426 kb on + strand at 3915.426 kb on + strand at 3915.628 kb on + strand, within Pf6N2E2_3385 at 3915.628 kb on + strand, within Pf6N2E2_3385 at 3915.628 kb on + strand, within Pf6N2E2_3385 at 3915.629 kb on - strand, within Pf6N2E2_3385 at 3915.629 kb on - strand, within Pf6N2E2_3385 at 3915.629 kb on - strand, within Pf6N2E2_3385 at 3915.629 kb on - strand, within Pf6N2E2_3385 at 3915.714 kb on + strand, within Pf6N2E2_3385 at 3915.715 kb on - strand, within Pf6N2E2_3385 at 3915.801 kb on + strand, within Pf6N2E2_3385 at 3915.801 kb on + strand, within Pf6N2E2_3385 at 3915.802 kb on - strand, within Pf6N2E2_3385 at 3915.802 kb on - strand, within Pf6N2E2_3385 at 3915.802 kb on - strand, within Pf6N2E2_3385 at 3915.802 kb on - strand, within Pf6N2E2_3385 at 3915.802 kb on - strand, within Pf6N2E2_3385 at 3915.802 kb on - strand, within Pf6N2E2_3385 at 3915.805 kb on + strand, within Pf6N2E2_3385 at 3915.806 kb on - strand, within Pf6N2E2_3385 at 3915.806 kb on - strand, within Pf6N2E2_3385 at 3915.806 kb on - strand, within Pf6N2E2_3385 at 3915.806 kb on - strand, within Pf6N2E2_3385 at 3915.806 kb on - strand, within Pf6N2E2_3385 at 3915.806 kb on - strand, within Pf6N2E2_3385 at 3915.870 kb on + strand, within Pf6N2E2_3385 at 3915.871 kb on - strand, within Pf6N2E2_3385 at 3915.931 kb on + strand, within Pf6N2E2_3385 at 3915.931 kb on + strand, within Pf6N2E2_3385 at 3915.931 kb on + strand, within Pf6N2E2_3385 at 3915.931 kb on + strand, within Pf6N2E2_3385 at 3915.931 kb on + strand, within Pf6N2E2_3385 at 3915.931 kb on + strand, within Pf6N2E2_3385 at 3915.932 kb on - strand, within Pf6N2E2_3385 at 3915.932 kb on - strand, within Pf6N2E2_3385 at 3915.932 kb on - strand, within Pf6N2E2_3385 at 3915.932 kb on - strand, within Pf6N2E2_3385 at 3915.932 kb on - strand, within Pf6N2E2_3385 at 3915.932 kb on - strand, within Pf6N2E2_3385 at 3915.977 kb on + strand, within Pf6N2E2_3385 at 3915.977 kb on + strand, within Pf6N2E2_3385 at 3916.170 kb on - strand, within Pf6N2E2_3385 at 3916.314 kb on + strand, within Pf6N2E2_3385 at 3916.414 kb on + strand, within Pf6N2E2_3385 at 3916.414 kb on + strand, within Pf6N2E2_3385 at 3916.414 kb on + strand, within Pf6N2E2_3385 at 3916.414 kb on + strand, within Pf6N2E2_3385 at 3916.415 kb on - strand, within Pf6N2E2_3385 at 3916.452 kb on + strand, within Pf6N2E2_3385 at 3916.453 kb on - strand, within Pf6N2E2_3385 at 3916.565 kb on + strand, within Pf6N2E2_3385 at 3916.566 kb on - strand, within Pf6N2E2_3385 at 3916.566 kb on - strand, within Pf6N2E2_3385 at 3916.909 kb on + strand, within Pf6N2E2_3385 at 3916.910 kb on - strand, within Pf6N2E2_3385 at 3916.915 kb on + strand, within Pf6N2E2_3385 at 3916.916 kb on - strand, within Pf6N2E2_3385 at 3916.916 kb on - strand, within Pf6N2E2_3385 at 3917.121 kb on - strand, within Pf6N2E2_3385 at 3917.266 kb on + strand, within Pf6N2E2_3385 at 3917.266 kb on + strand, within Pf6N2E2_3385 at 3917.266 kb on + strand, within Pf6N2E2_3385 at 3917.267 kb on - strand, within Pf6N2E2_3385 at 3917.313 kb on + strand, within Pf6N2E2_3385 at 3917.314 kb on - strand, within Pf6N2E2_3385 at 3917.515 kb on + strand, within Pf6N2E2_3385 at 3917.515 kb on + strand, within Pf6N2E2_3385 at 3917.516 kb on - strand, within Pf6N2E2_3385 at 3917.581 kb on + strand, within Pf6N2E2_3385 at 3917.581 kb on + strand, within Pf6N2E2_3385 at 3917.581 kb on + strand, within Pf6N2E2_3385 at 3917.581 kb on + strand, within Pf6N2E2_3385 at 3917.728 kb on - strand at 3917.837 kb on + strand at 3917.837 kb on + strand at 3917.890 kb on - strand at 3917.926 kb on + strand at 3917.926 kb on + strand at 3917.927 kb on - strand at 3917.927 kb on - strand at 3917.927 kb on - strand at 3917.927 kb on - strand at 3917.972 kb on + strand at 3917.972 kb on + strand at 3917.972 kb on + strand at 3917.973 kb on - strand at 3917.973 kb on - strand at 3918.105 kb on + strand at 3918.105 kb on + strand at 3918.105 kb on + strand at 3918.106 kb on - strand at 3918.106 kb on - strand at 3918.106 kb on - strand at 3918.106 kb on - strand at 3918.111 kb on + strand at 3918.111 kb on + strand at 3918.112 kb on - strand at 3918.112 kb on - strand at 3918.172 kb on + strand, within Pf6N2E2_3386 at 3918.173 kb on - strand, within Pf6N2E2_3386 at 3918.173 kb on - strand, within Pf6N2E2_3386 at 3918.173 kb on - strand, within Pf6N2E2_3386 at 3918.210 kb on - strand, within Pf6N2E2_3386 at 3918.210 kb on - strand, within Pf6N2E2_3386 at 3918.210 kb on - strand, within Pf6N2E2_3386 at 3918.232 kb on + strand, within Pf6N2E2_3386 at 3918.233 kb on - strand, within Pf6N2E2_3386 at 3918.358 kb on + strand at 3918.358 kb on + strand at 3918.358 kb on + strand at 3918.359 kb on - strand at 3918.871 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 3,914,271 + Pf6N2E2_3383 0.70 -2.6 3,914,272 - Pf6N2E2_3383 0.70 -2.8 3,914,572 - Pf6N2E2_3384 0.13 -4.5 3,914,572 - Pf6N2E2_3384 0.13 -3.6 3,914,585 + Pf6N2E2_3384 0.15 -2.4 3,915,002 + Pf6N2E2_3384 0.77 -3.0 3,915,002 + Pf6N2E2_3384 0.77 -3.5 3,915,002 + Pf6N2E2_3384 0.77 -3.1 3,915,003 - Pf6N2E2_3384 0.77 -4.2 3,915,003 - Pf6N2E2_3384 0.77 -2.6 3,915,003 - Pf6N2E2_3384 0.77 -3.5 3,915,176 + -3.8 3,915,176 + -1.5 3,915,176 + -3.0 3,915,176 + -3.3 3,915,177 - -2.4 3,915,288 - -0.5 3,915,426 + -2.5 3,915,426 + -3.5 3,915,628 + Pf6N2E2_3385 0.17 -4.0 3,915,628 + Pf6N2E2_3385 0.17 -2.8 3,915,628 + Pf6N2E2_3385 0.17 -3.2 3,915,629 - Pf6N2E2_3385 0.17 -3.3 3,915,629 - Pf6N2E2_3385 0.17 -4.4 3,915,629 - Pf6N2E2_3385 0.17 -4.5 3,915,629 - Pf6N2E2_3385 0.17 -5.0 3,915,714 + Pf6N2E2_3385 0.20 -1.1 3,915,715 - Pf6N2E2_3385 0.20 -2.7 3,915,801 + Pf6N2E2_3385 0.23 -4.1 3,915,801 + Pf6N2E2_3385 0.23 -1.7 3,915,802 - Pf6N2E2_3385 0.23 -4.3 3,915,802 - Pf6N2E2_3385 0.23 -3.0 3,915,802 - Pf6N2E2_3385 0.23 -4.2 3,915,802 - Pf6N2E2_3385 0.23 -2.9 3,915,802 - Pf6N2E2_3385 0.23 -4.6 3,915,802 - Pf6N2E2_3385 0.23 -0.8 3,915,805 + Pf6N2E2_3385 0.23 -3.5 3,915,806 - Pf6N2E2_3385 0.23 -3.7 3,915,806 - Pf6N2E2_3385 0.23 -3.0 3,915,806 - Pf6N2E2_3385 0.23 -3.1 3,915,806 - Pf6N2E2_3385 0.23 -4.1 3,915,806 - Pf6N2E2_3385 0.23 -2.6 3,915,806 - Pf6N2E2_3385 0.23 -4.6 3,915,870 + Pf6N2E2_3385 0.25 -3.5 3,915,871 - Pf6N2E2_3385 0.26 -3.9 3,915,931 + Pf6N2E2_3385 0.28 -0.7 3,915,931 + Pf6N2E2_3385 0.28 -1.0 3,915,931 + Pf6N2E2_3385 0.28 -3.3 3,915,931 + Pf6N2E2_3385 0.28 -4.1 3,915,931 + Pf6N2E2_3385 0.28 -1.5 3,915,931 + Pf6N2E2_3385 0.28 -4.0 3,915,932 - Pf6N2E2_3385 0.28 -2.2 3,915,932 - Pf6N2E2_3385 0.28 -0.7 3,915,932 - Pf6N2E2_3385 0.28 -1.3 3,915,932 - Pf6N2E2_3385 0.28 -2.5 3,915,932 - Pf6N2E2_3385 0.28 -4.3 3,915,932 - Pf6N2E2_3385 0.28 -2.6 3,915,977 + Pf6N2E2_3385 0.29 -1.4 3,915,977 + Pf6N2E2_3385 0.29 -3.3 3,916,170 - Pf6N2E2_3385 0.36 -3.4 3,916,314 + Pf6N2E2_3385 0.41 -2.5 3,916,414 + Pf6N2E2_3385 0.45 -1.5 3,916,414 + Pf6N2E2_3385 0.45 -1.0 3,916,414 + Pf6N2E2_3385 0.45 -4.3 3,916,414 + Pf6N2E2_3385 0.45 -1.5 3,916,415 - Pf6N2E2_3385 0.45 -1.7 3,916,452 + Pf6N2E2_3385 0.46 -2.9 3,916,453 - Pf6N2E2_3385 0.46 -4.2 3,916,565 + Pf6N2E2_3385 0.50 -0.7 3,916,566 - Pf6N2E2_3385 0.50 -1.6 3,916,566 - Pf6N2E2_3385 0.50 -3.0 3,916,909 + Pf6N2E2_3385 0.62 -4.6 3,916,910 - Pf6N2E2_3385 0.62 -2.1 3,916,915 + Pf6N2E2_3385 0.63 -3.6 3,916,916 - Pf6N2E2_3385 0.63 -4.4 3,916,916 - Pf6N2E2_3385 0.63 -2.5 3,917,121 - Pf6N2E2_3385 0.70 -3.5 3,917,266 + Pf6N2E2_3385 0.75 -3.0 3,917,266 + Pf6N2E2_3385 0.75 -3.8 3,917,266 + Pf6N2E2_3385 0.75 -2.9 3,917,267 - Pf6N2E2_3385 0.75 -1.1 3,917,313 + Pf6N2E2_3385 0.77 -2.3 3,917,314 - Pf6N2E2_3385 0.77 -4.3 3,917,515 + Pf6N2E2_3385 0.84 -2.5 3,917,515 + Pf6N2E2_3385 0.84 -3.9 3,917,516 - Pf6N2E2_3385 0.84 -2.4 3,917,581 + Pf6N2E2_3385 0.86 -1.7 3,917,581 + Pf6N2E2_3385 0.86 -3.1 3,917,581 + Pf6N2E2_3385 0.86 -2.3 3,917,581 + Pf6N2E2_3385 0.86 -3.4 3,917,728 - -2.8 3,917,837 + -4.0 3,917,837 + -3.8 3,917,890 - -2.3 3,917,926 + -2.3 3,917,926 + -3.5 3,917,927 - -4.1 3,917,927 - -3.6 3,917,927 - -3.1 3,917,927 - -3.9 3,917,972 + -1.8 3,917,972 + -1.1 3,917,972 + -0.9 3,917,973 - -0.0 3,917,973 - -1.1 3,918,105 + +0.9 3,918,105 + +1.2 3,918,105 + -0.3 3,918,106 - -0.7 3,918,106 - -2.0 3,918,106 - -0.2 3,918,106 - -0.5 3,918,111 + +0.3 3,918,111 + -1.0 3,918,112 - -0.7 3,918,112 - -1.6 3,918,172 + Pf6N2E2_3386 0.29 -1.0 3,918,173 - Pf6N2E2_3386 0.29 +0.8 3,918,173 - Pf6N2E2_3386 0.29 -0.2 3,918,173 - Pf6N2E2_3386 0.29 +0.2 3,918,210 - Pf6N2E2_3386 0.42 +0.5 3,918,210 - Pf6N2E2_3386 0.42 -2.4 3,918,210 - Pf6N2E2_3386 0.42 -3.3 3,918,232 + Pf6N2E2_3386 0.50 -0.5 3,918,233 - Pf6N2E2_3386 0.51 -0.9 3,918,358 + -0.1 3,918,358 + +0.0 3,918,358 + +0.1 3,918,359 - -0.2 3,918,871 - -2.3
Or see this region's nucleotide sequence