Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3381 and Pf6N2E2_3382 overlap by 4 nucleotides Pf6N2E2_3382 and Pf6N2E2_3383 are separated by 167 nucleotides Pf6N2E2_3383 and Pf6N2E2_3384 overlap by 4 nucleotides Pf6N2E2_3384 and Pf6N2E2_3385 overlap by 4 nucleotides
Pf6N2E2_3381 - Predicted L-lactate dehydrogenase, Fe-S oxidoreductase subunit YkgE, at 3,912,209 to 3,913,033
_3381
Pf6N2E2_3382 - Predicted L-lactate dehydrogenase, Iron-sulfur cluster-binding subunit YkgF, at 3,913,030 to 3,913,593
_3382
Pf6N2E2_3383 - Predicted L-lactate dehydrogenase, Iron-sulfur cluster-binding subunit YkgF, at 3,913,761 to 3,914,489
_3383
Pf6N2E2_3384 - Predicted L-lactate dehydrogenase, hypothetical protein subunit YkgG, at 3,914,486 to 3,915,157
_3384
Pf6N2E2_3385 - Predicted D-lactate dehydrogenase, Fe-S protein, FAD/FMN-containing, at 3,915,154 to 3,917,964
_3385
Position (kb)
3913
3914
3915 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3913.469 kb on + strand, within Pf6N2E2_3382 at 3913.469 kb on + strand, within Pf6N2E2_3382 at 3913.469 kb on + strand, within Pf6N2E2_3382 at 3913.469 kb on + strand, within Pf6N2E2_3382 at 3913.469 kb on + strand, within Pf6N2E2_3382 at 3913.470 kb on - strand, within Pf6N2E2_3382 at 3913.470 kb on - strand, within Pf6N2E2_3382 at 3913.470 kb on - strand, within Pf6N2E2_3382 at 3913.470 kb on - strand, within Pf6N2E2_3382 at 3913.470 kb on - strand, within Pf6N2E2_3382 at 3913.510 kb on + strand, within Pf6N2E2_3382 at 3913.510 kb on + strand, within Pf6N2E2_3382 at 3913.510 kb on + strand, within Pf6N2E2_3382 at 3913.510 kb on + strand, within Pf6N2E2_3382 at 3913.510 kb on + strand, within Pf6N2E2_3382 at 3913.510 kb on + strand, within Pf6N2E2_3382 at 3913.510 kb on + strand, within Pf6N2E2_3382 at 3913.511 kb on - strand, within Pf6N2E2_3382 at 3913.511 kb on - strand, within Pf6N2E2_3382 at 3913.511 kb on - strand, within Pf6N2E2_3382 at 3913.578 kb on - strand at 3913.578 kb on - strand at 3913.620 kb on + strand at 3913.620 kb on + strand at 3913.620 kb on + strand at 3913.621 kb on - strand at 3913.621 kb on - strand at 3913.621 kb on - strand at 3913.857 kb on + strand, within Pf6N2E2_3383 at 3913.857 kb on + strand, within Pf6N2E2_3383 at 3913.858 kb on - strand, within Pf6N2E2_3383 at 3913.858 kb on - strand, within Pf6N2E2_3383 at 3913.858 kb on - strand, within Pf6N2E2_3383 at 3914.006 kb on + strand, within Pf6N2E2_3383 at 3914.006 kb on + strand, within Pf6N2E2_3383 at 3914.007 kb on - strand, within Pf6N2E2_3383 at 3914.007 kb on - strand, within Pf6N2E2_3383 at 3914.025 kb on + strand at 3914.025 kb on + strand, within Pf6N2E2_3383 at 3914.026 kb on - strand, within Pf6N2E2_3383 at 3914.026 kb on - strand, within Pf6N2E2_3383 at 3914.062 kb on - strand, within Pf6N2E2_3383 at 3914.073 kb on - strand, within Pf6N2E2_3383 at 3914.271 kb on + strand, within Pf6N2E2_3383 at 3914.272 kb on - strand, within Pf6N2E2_3383 at 3914.572 kb on - strand, within Pf6N2E2_3384 at 3914.572 kb on - strand, within Pf6N2E2_3384 at 3914.585 kb on + strand, within Pf6N2E2_3384 at 3915.002 kb on + strand, within Pf6N2E2_3384 at 3915.002 kb on + strand, within Pf6N2E2_3384 at 3915.002 kb on + strand, within Pf6N2E2_3384 at 3915.003 kb on - strand, within Pf6N2E2_3384 at 3915.003 kb on - strand, within Pf6N2E2_3384 at 3915.003 kb on - strand, within Pf6N2E2_3384 at 3915.176 kb on + strand at 3915.176 kb on + strand at 3915.176 kb on + strand at 3915.176 kb on + strand at 3915.177 kb on - strand at 3915.288 kb on - strand at 3915.426 kb on + strand at 3915.426 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 3,913,469 + Pf6N2E2_3382 0.78 -1.7 3,913,469 + Pf6N2E2_3382 0.78 -3.9 3,913,469 + Pf6N2E2_3382 0.78 -1.7 3,913,469 + Pf6N2E2_3382 0.78 -3.0 3,913,469 + Pf6N2E2_3382 0.78 -2.8 3,913,470 - Pf6N2E2_3382 0.78 -2.0 3,913,470 - Pf6N2E2_3382 0.78 -2.5 3,913,470 - Pf6N2E2_3382 0.78 -1.0 3,913,470 - Pf6N2E2_3382 0.78 -3.3 3,913,470 - Pf6N2E2_3382 0.78 -3.4 3,913,510 + Pf6N2E2_3382 0.85 -4.2 3,913,510 + Pf6N2E2_3382 0.85 -4.0 3,913,510 + Pf6N2E2_3382 0.85 -3.0 3,913,510 + Pf6N2E2_3382 0.85 -2.5 3,913,510 + Pf6N2E2_3382 0.85 -3.9 3,913,510 + Pf6N2E2_3382 0.85 -4.1 3,913,510 + Pf6N2E2_3382 0.85 -3.5 3,913,511 - Pf6N2E2_3382 0.85 -2.6 3,913,511 - Pf6N2E2_3382 0.85 -0.2 3,913,511 - Pf6N2E2_3382 0.85 -3.8 3,913,578 - -4.2 3,913,578 - -3.5 3,913,620 + -3.7 3,913,620 + -3.9 3,913,620 + -3.7 3,913,621 - -3.3 3,913,621 - -4.0 3,913,621 - -3.4 3,913,857 + Pf6N2E2_3383 0.13 -3.4 3,913,857 + Pf6N2E2_3383 0.13 -0.8 3,913,858 - Pf6N2E2_3383 0.13 -3.3 3,913,858 - Pf6N2E2_3383 0.13 -4.0 3,913,858 - Pf6N2E2_3383 0.13 -3.8 3,914,006 + Pf6N2E2_3383 0.34 -2.9 3,914,006 + Pf6N2E2_3383 0.34 -3.8 3,914,007 - Pf6N2E2_3383 0.34 -3.6 3,914,007 - Pf6N2E2_3383 0.34 -2.9 3,914,025 + -0.3 3,914,025 + Pf6N2E2_3383 0.36 -3.3 3,914,026 - Pf6N2E2_3383 0.36 -2.8 3,914,026 - Pf6N2E2_3383 0.36 -3.2 3,914,062 - Pf6N2E2_3383 0.41 -2.9 3,914,073 - Pf6N2E2_3383 0.43 -3.3 3,914,271 + Pf6N2E2_3383 0.70 -2.6 3,914,272 - Pf6N2E2_3383 0.70 -2.8 3,914,572 - Pf6N2E2_3384 0.13 -4.5 3,914,572 - Pf6N2E2_3384 0.13 -3.6 3,914,585 + Pf6N2E2_3384 0.15 -2.4 3,915,002 + Pf6N2E2_3384 0.77 -3.0 3,915,002 + Pf6N2E2_3384 0.77 -3.5 3,915,002 + Pf6N2E2_3384 0.77 -3.1 3,915,003 - Pf6N2E2_3384 0.77 -4.2 3,915,003 - Pf6N2E2_3384 0.77 -2.6 3,915,003 - Pf6N2E2_3384 0.77 -3.5 3,915,176 + -3.8 3,915,176 + -1.5 3,915,176 + -3.0 3,915,176 + -3.3 3,915,177 - -2.4 3,915,288 - -0.5 3,915,426 + -2.5 3,915,426 + -3.5
Or see this region's nucleotide sequence