Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2318 and Pf6N2E2_2319 are separated by 24 nucleotides Pf6N2E2_2319 and Pf6N2E2_2320 overlap by 8 nucleotides Pf6N2E2_2320 and Pf6N2E2_2321 are separated by 120 nucleotides
Pf6N2E2_2318 - Type cbb3 cytochrome oxidase biogenesis protein CcoI; Copper-translocating P-type ATPase (EC 3.6.3.4), at 2,740,743 to 2,743,193
_2318
Pf6N2E2_2319 - Type cbb3 cytochrome oxidase biogenesis protein CcoS, involved in heme b insertion, at 2,743,218 to 2,743,436
_2319
Pf6N2E2_2320 - Heavy-metal-associated domain (N-terminus) and membrane-bounded cytochrome biogenesis cycZ-like domain, possible membrane copper tolerance protein, at 2,743,429 to 2,744,112
_2320
Pf6N2E2_2321 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 2,744,233 to 2,745,615
_2321
Position (kb)
2743
2744
2745 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2743.020 kb on + strand at 2743.020 kb on + strand at 2743.020 kb on + strand at 2743.021 kb on - strand at 2743.021 kb on - strand at 2743.021 kb on - strand at 2743.021 kb on - strand at 2743.062 kb on - strand at 2743.334 kb on + strand, within Pf6N2E2_2319 at 2743.582 kb on + strand, within Pf6N2E2_2320 at 2743.582 kb on + strand, within Pf6N2E2_2320 at 2743.606 kb on + strand, within Pf6N2E2_2320 at 2743.607 kb on - strand, within Pf6N2E2_2320 at 2743.607 kb on - strand, within Pf6N2E2_2320 at 2743.717 kb on + strand, within Pf6N2E2_2320 at 2743.718 kb on - strand, within Pf6N2E2_2320 at 2744.194 kb on + strand at 2744.195 kb on - strand at 2744.296 kb on + strand at 2744.402 kb on - strand, within Pf6N2E2_2321 at 2744.412 kb on - strand, within Pf6N2E2_2321 at 2744.412 kb on - strand, within Pf6N2E2_2321 at 2744.434 kb on + strand, within Pf6N2E2_2321 at 2744.435 kb on - strand, within Pf6N2E2_2321 at 2744.438 kb on - strand, within Pf6N2E2_2321 at 2744.438 kb on - strand, within Pf6N2E2_2321 at 2744.438 kb on - strand, within Pf6N2E2_2321 at 2744.488 kb on + strand, within Pf6N2E2_2321 at 2744.669 kb on - strand, within Pf6N2E2_2321 at 2744.866 kb on - strand, within Pf6N2E2_2321 at 2744.968 kb on + strand, within Pf6N2E2_2321 at 2744.969 kb on - strand, within Pf6N2E2_2321 at 2744.969 kb on - strand, within Pf6N2E2_2321 at 2745.094 kb on + strand, within Pf6N2E2_2321 at 2745.095 kb on - strand, within Pf6N2E2_2321 at 2745.095 kb on - strand, within Pf6N2E2_2321
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 2,743,020 + -0.8 2,743,020 + -2.2 2,743,020 + -1.5 2,743,021 - -0.8 2,743,021 - +0.6 2,743,021 - +0.6 2,743,021 - -2.9 2,743,062 - -2.2 2,743,334 + Pf6N2E2_2319 0.53 -0.2 2,743,582 + Pf6N2E2_2320 0.22 +0.2 2,743,582 + Pf6N2E2_2320 0.22 -0.4 2,743,606 + Pf6N2E2_2320 0.26 -0.5 2,743,607 - Pf6N2E2_2320 0.26 -0.6 2,743,607 - Pf6N2E2_2320 0.26 -1.2 2,743,717 + Pf6N2E2_2320 0.42 -2.5 2,743,718 - Pf6N2E2_2320 0.42 -1.6 2,744,194 + +2.2 2,744,195 - +0.2 2,744,296 + -0.2 2,744,402 - Pf6N2E2_2321 0.12 -0.5 2,744,412 - Pf6N2E2_2321 0.13 +1.6 2,744,412 - Pf6N2E2_2321 0.13 +0.0 2,744,434 + Pf6N2E2_2321 0.15 +0.5 2,744,435 - Pf6N2E2_2321 0.15 +0.4 2,744,438 - Pf6N2E2_2321 0.15 +0.2 2,744,438 - Pf6N2E2_2321 0.15 -0.0 2,744,438 - Pf6N2E2_2321 0.15 +0.2 2,744,488 + Pf6N2E2_2321 0.18 +0.5 2,744,669 - Pf6N2E2_2321 0.32 -0.7 2,744,866 - Pf6N2E2_2321 0.46 -0.3 2,744,968 + Pf6N2E2_2321 0.53 +2.4 2,744,969 - Pf6N2E2_2321 0.53 -0.9 2,744,969 - Pf6N2E2_2321 0.53 +0.2 2,745,094 + Pf6N2E2_2321 0.62 -1.0 2,745,095 - Pf6N2E2_2321 0.62 +1.1 2,745,095 - Pf6N2E2_2321 0.62 +0.8
Or see this region's nucleotide sequence