Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04810 and MMJJ_RS04815 are separated by 168 nucleotides MMJJ_RS04815 and MMJJ_RS04820 are separated by 33 nucleotides MMJJ_RS04820 and MMJJ_RS04825 are separated by 8 nucleotides
MMJJ_RS04810: MMJJ_RS04810 - nitroreductase family protein, at 901,592 to 902,113
_RS04810
MMJJ_RS04815: MMJJ_RS04815 - TIM barrel protein, at 902,282 to 902,938
_RS04815
MMJJ_RS04820: MMJJ_RS04820 - hypothetical protein, at 902,972 to 903,781
_RS04820
MMJJ_RS04825: MMJJ_RS04825 - 2,3-bisphosphoglycerate-independent phosphoglycerate mutase, at 903,790 to 905,070
_RS04825
Position (kb) 902
903
904 Strain fitness (log2 ratio) -2
-1
0
1 at 901.977 kb on + strand, within MMJJ_RS04810 at 902.048 kb on - strand, within MMJJ_RS04810 at 902.076 kb on - strand at 902.076 kb on - strand at 902.077 kb on + strand at 902.102 kb on - strand at 902.113 kb on - strand at 902.113 kb on - strand at 902.114 kb on + strand at 902.114 kb on + strand at 902.115 kb on - strand at 902.115 kb on - strand at 902.143 kb on + strand at 902.147 kb on + strand at 902.170 kb on - strand at 902.171 kb on + strand at 902.171 kb on + strand at 902.172 kb on - strand at 902.172 kb on - strand at 902.172 kb on - strand at 902.196 kb on - strand at 902.198 kb on - strand at 902.198 kb on - strand at 902.199 kb on + strand at 902.199 kb on + strand at 902.199 kb on + strand at 902.199 kb on + strand at 902.247 kb on + strand at 902.281 kb on - strand at 902.282 kb on + strand at 902.287 kb on + strand at 902.332 kb on - strand at 902.413 kb on + strand, within MMJJ_RS04815 at 902.471 kb on + strand, within MMJJ_RS04815 at 902.479 kb on - strand, within MMJJ_RS04815 at 902.494 kb on + strand, within MMJJ_RS04815 at 902.498 kb on + strand, within MMJJ_RS04815 at 902.567 kb on - strand, within MMJJ_RS04815 at 902.641 kb on + strand, within MMJJ_RS04815 at 902.686 kb on + strand, within MMJJ_RS04815 at 902.759 kb on - strand, within MMJJ_RS04815 at 902.759 kb on - strand, within MMJJ_RS04815 at 902.839 kb on + strand, within MMJJ_RS04815 at 902.872 kb on - strand, within MMJJ_RS04815 at 902.914 kb on - strand at 902.915 kb on + strand at 902.915 kb on + strand at 902.918 kb on - strand at 902.936 kb on - strand at 902.937 kb on + strand at 902.946 kb on - strand at 902.946 kb on - strand at 902.947 kb on + strand at 902.947 kb on + strand at 902.947 kb on + strand at 902.953 kb on + strand at 902.957 kb on - strand at 902.957 kb on - strand at 902.961 kb on - strand at 902.961 kb on - strand at 902.961 kb on - strand at 902.962 kb on + strand at 902.962 kb on + strand at 902.962 kb on + strand at 903.006 kb on - strand at 903.109 kb on - strand, within MMJJ_RS04820 at 903.213 kb on - strand, within MMJJ_RS04820 at 903.214 kb on + strand, within MMJJ_RS04820 at 903.221 kb on + strand, within MMJJ_RS04820 at 903.248 kb on + strand, within MMJJ_RS04820 at 903.313 kb on - strand, within MMJJ_RS04820 at 903.344 kb on - strand, within MMJJ_RS04820 at 903.371 kb on - strand, within MMJJ_RS04820 at 903.371 kb on - strand, within MMJJ_RS04820 at 903.385 kb on + strand, within MMJJ_RS04820 at 903.421 kb on + strand, within MMJJ_RS04820 at 903.439 kb on + strand, within MMJJ_RS04820 at 903.491 kb on - strand, within MMJJ_RS04820 at 903.491 kb on - strand, within MMJJ_RS04820 at 903.491 kb on - strand, within MMJJ_RS04820 at 903.492 kb on + strand, within MMJJ_RS04820 at 903.492 kb on + strand, within MMJJ_RS04820 at 903.494 kb on - strand, within MMJJ_RS04820 at 903.496 kb on - strand, within MMJJ_RS04820 at 903.496 kb on - strand, within MMJJ_RS04820 at 903.496 kb on - strand, within MMJJ_RS04820 at 903.519 kb on - strand, within MMJJ_RS04820 at 903.520 kb on + strand, within MMJJ_RS04820 at 903.687 kb on - strand, within MMJJ_RS04820 at 903.697 kb on - strand, within MMJJ_RS04820 at 903.697 kb on - strand, within MMJJ_RS04820 at 903.699 kb on - strand, within MMJJ_RS04820 at 903.699 kb on - strand, within MMJJ_RS04820 at 903.700 kb on + strand, within MMJJ_RS04820 at 903.700 kb on + strand, within MMJJ_RS04820 at 903.702 kb on + strand at 903.734 kb on - strand at 903.736 kb on - strand at 903.736 kb on - strand at 903.737 kb on + strand at 903.737 kb on + strand at 903.740 kb on + strand at 903.748 kb on - strand at 903.748 kb on - strand at 903.749 kb on + strand at 903.752 kb on - strand at 903.752 kb on - strand at 903.806 kb on - strand at 903.810 kb on + strand at 903.813 kb on + strand at 903.852 kb on - strand at 903.887 kb on - strand at 903.933 kb on - strand, within MMJJ_RS04825 at 903.934 kb on + strand, within MMJJ_RS04825 at 903.993 kb on + strand, within MMJJ_RS04825 at 904.059 kb on - strand, within MMJJ_RS04825 at 904.059 kb on - strand, within MMJJ_RS04825 at 904.063 kb on - strand, within MMJJ_RS04825 at 904.064 kb on + strand, within MMJJ_RS04825 at 904.084 kb on - strand, within MMJJ_RS04825 at 904.088 kb on - strand, within MMJJ_RS04825 at 904.119 kb on - strand, within MMJJ_RS04825 at 904.119 kb on - strand, within MMJJ_RS04825 at 904.125 kb on - strand, within MMJJ_RS04825 at 904.134 kb on - strand, within MMJJ_RS04825 at 904.137 kb on + strand, within MMJJ_RS04825 at 904.151 kb on - strand, within MMJJ_RS04825 at 904.152 kb on + strand, within MMJJ_RS04825 at 904.162 kb on + strand, within MMJJ_RS04825 at 904.163 kb on - strand, within MMJJ_RS04825 at 904.163 kb on - strand, within MMJJ_RS04825 at 904.164 kb on + strand, within MMJJ_RS04825 at 904.200 kb on - strand, within MMJJ_RS04825 at 904.205 kb on - strand, within MMJJ_RS04825 at 904.206 kb on + strand, within MMJJ_RS04825 at 904.206 kb on + strand, within MMJJ_RS04825 at 904.206 kb on + strand, within MMJJ_RS04825 at 904.206 kb on + strand, within MMJJ_RS04825 at 904.206 kb on + strand, within MMJJ_RS04825 at 904.206 kb on + strand, within MMJJ_RS04825 at 904.210 kb on - strand, within MMJJ_RS04825 at 904.211 kb on + strand, within MMJJ_RS04825 at 904.211 kb on + strand, within MMJJ_RS04825 at 904.221 kb on - strand, within MMJJ_RS04825 at 904.222 kb on + strand, within MMJJ_RS04825 at 904.231 kb on + strand, within MMJJ_RS04825 at 904.242 kb on + strand, within MMJJ_RS04825 at 904.269 kb on - strand, within MMJJ_RS04825 at 904.274 kb on - strand, within MMJJ_RS04825 at 904.349 kb on - strand, within MMJJ_RS04825 at 904.390 kb on + strand, within MMJJ_RS04825 at 904.390 kb on + strand, within MMJJ_RS04825 at 904.393 kb on - strand, within MMJJ_RS04825 at 904.438 kb on + strand, within MMJJ_RS04825 at 904.482 kb on - strand, within MMJJ_RS04825 at 904.483 kb on + strand, within MMJJ_RS04825 at 904.503 kb on - strand, within MMJJ_RS04825 at 904.503 kb on - strand, within MMJJ_RS04825 at 904.504 kb on + strand, within MMJJ_RS04825 at 904.511 kb on - strand, within MMJJ_RS04825 at 904.512 kb on + strand, within MMJJ_RS04825 at 904.527 kb on - strand, within MMJJ_RS04825 at 904.527 kb on - strand, within MMJJ_RS04825 at 904.528 kb on + strand, within MMJJ_RS04825 at 904.528 kb on + strand, within MMJJ_RS04825 at 904.603 kb on - strand, within MMJJ_RS04825 at 904.604 kb on + strand, within MMJJ_RS04825 at 904.707 kb on - strand, within MMJJ_RS04825 at 904.725 kb on - strand, within MMJJ_RS04825 at 904.726 kb on + strand, within MMJJ_RS04825 at 904.752 kb on - strand, within MMJJ_RS04825 at 904.752 kb on - strand, within MMJJ_RS04825 at 904.753 kb on + strand, within MMJJ_RS04825
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE remove 901,977 + MMJJ_RS04810 0.74 +0.2 902,048 - MMJJ_RS04810 0.87 -0.1 902,076 - -0.2 902,076 - -1.6 902,077 + +0.2 902,102 - +0.1 902,113 - +0.3 902,113 - +0.0 902,114 + +0.2 902,114 + +0.9 902,115 - +0.1 902,115 - -1.6 902,143 + -0.3 902,147 + +0.4 902,170 - -0.4 902,171 + -0.2 902,171 + -0.0 902,172 - -0.7 902,172 - +0.1 902,172 - -0.7 902,196 - -0.1 902,198 - +0.2 902,198 - +0.0 902,199 + +0.3 902,199 + +0.0 902,199 + -0.9 902,199 + +0.2 902,247 + -0.1 902,281 - -0.6 902,282 + +0.2 902,287 + +0.4 902,332 - -0.4 902,413 + MMJJ_RS04815 0.20 -0.0 902,471 + MMJJ_RS04815 0.29 -0.3 902,479 - MMJJ_RS04815 0.30 -0.7 902,494 + MMJJ_RS04815 0.32 -0.5 902,498 + MMJJ_RS04815 0.33 +1.0 902,567 - MMJJ_RS04815 0.43 +0.3 902,641 + MMJJ_RS04815 0.55 +0.0 902,686 + MMJJ_RS04815 0.61 -1.5 902,759 - MMJJ_RS04815 0.73 +0.1 902,759 - MMJJ_RS04815 0.73 -0.4 902,839 + MMJJ_RS04815 0.85 +0.3 902,872 - MMJJ_RS04815 0.90 -0.1 902,914 - +0.1 902,915 + -0.4 902,915 + -0.7 902,918 - +0.6 902,936 - +0.9 902,937 + -0.2 902,946 - +0.5 902,946 - -0.7 902,947 + -0.1 902,947 + +0.1 902,947 + +0.1 902,953 + -0.0 902,957 - -0.2 902,957 - -0.3 902,961 - +0.4 902,961 - -0.4 902,961 - +0.0 902,962 + -1.3 902,962 + +1.1 902,962 + +0.1 903,006 - +1.7 903,109 - MMJJ_RS04820 0.17 +1.0 903,213 - MMJJ_RS04820 0.30 -0.1 903,214 + MMJJ_RS04820 0.30 +0.6 903,221 + MMJJ_RS04820 0.31 +1.3 903,248 + MMJJ_RS04820 0.34 +0.3 903,313 - MMJJ_RS04820 0.42 -0.0 903,344 - MMJJ_RS04820 0.46 +0.2 903,371 - MMJJ_RS04820 0.49 +0.3 903,371 - MMJJ_RS04820 0.49 +0.6 903,385 + MMJJ_RS04820 0.51 -0.5 903,421 + MMJJ_RS04820 0.55 -0.1 903,439 + MMJJ_RS04820 0.58 +0.0 903,491 - MMJJ_RS04820 0.64 -0.4 903,491 - MMJJ_RS04820 0.64 +0.8 903,491 - MMJJ_RS04820 0.64 +0.9 903,492 + MMJJ_RS04820 0.64 +0.2 903,492 + MMJJ_RS04820 0.64 +1.1 903,494 - MMJJ_RS04820 0.64 +0.2 903,496 - MMJJ_RS04820 0.65 +0.8 903,496 - MMJJ_RS04820 0.65 +0.1 903,496 - MMJJ_RS04820 0.65 +0.1 903,519 - MMJJ_RS04820 0.68 -0.3 903,520 + MMJJ_RS04820 0.68 -0.8 903,687 - MMJJ_RS04820 0.88 +0.0 903,697 - MMJJ_RS04820 0.90 -0.4 903,697 - MMJJ_RS04820 0.90 +0.3 903,699 - MMJJ_RS04820 0.90 +0.3 903,699 - MMJJ_RS04820 0.90 +0.1 903,700 + MMJJ_RS04820 0.90 -0.2 903,700 + MMJJ_RS04820 0.90 -0.1 903,702 + -1.9 903,734 - -0.3 903,736 - -1.4 903,736 - +1.4 903,737 + -0.7 903,737 + -0.7 903,740 + +1.1 903,748 - +0.1 903,748 - +0.4 903,749 + -0.8 903,752 - +0.1 903,752 - +0.6 903,806 - +0.8 903,810 + +0.1 903,813 + -0.5 903,852 - +0.1 903,887 - +0.5 903,933 - MMJJ_RS04825 0.11 -0.3 903,934 + MMJJ_RS04825 0.11 +0.1 903,993 + MMJJ_RS04825 0.16 -0.1 904,059 - MMJJ_RS04825 0.21 -0.4 904,059 - MMJJ_RS04825 0.21 -0.1 904,063 - MMJJ_RS04825 0.21 +0.9 904,064 + MMJJ_RS04825 0.21 -0.2 904,084 - MMJJ_RS04825 0.23 -0.6 904,088 - MMJJ_RS04825 0.23 +0.5 904,119 - MMJJ_RS04825 0.26 -0.0 904,119 - MMJJ_RS04825 0.26 +1.1 904,125 - MMJJ_RS04825 0.26 +0.6 904,134 - MMJJ_RS04825 0.27 +0.0 904,137 + MMJJ_RS04825 0.27 +0.3 904,151 - MMJJ_RS04825 0.28 -0.1 904,152 + MMJJ_RS04825 0.28 +1.2 904,162 + MMJJ_RS04825 0.29 -0.2 904,163 - MMJJ_RS04825 0.29 +0.8 904,163 - MMJJ_RS04825 0.29 +0.2 904,164 + MMJJ_RS04825 0.29 +0.3 904,200 - MMJJ_RS04825 0.32 -0.2 904,205 - MMJJ_RS04825 0.32 +0.3 904,206 + MMJJ_RS04825 0.32 +0.1 904,206 + MMJJ_RS04825 0.32 +0.7 904,206 + MMJJ_RS04825 0.32 -0.0 904,206 + MMJJ_RS04825 0.32 +0.8 904,206 + MMJJ_RS04825 0.32 +0.1 904,206 + MMJJ_RS04825 0.32 -0.4 904,210 - MMJJ_RS04825 0.33 +0.1 904,211 + MMJJ_RS04825 0.33 +0.2 904,211 + MMJJ_RS04825 0.33 -0.5 904,221 - MMJJ_RS04825 0.34 -0.8 904,222 + MMJJ_RS04825 0.34 +1.3 904,231 + MMJJ_RS04825 0.34 -0.2 904,242 + MMJJ_RS04825 0.35 +0.2 904,269 - MMJJ_RS04825 0.37 +0.3 904,274 - MMJJ_RS04825 0.38 -0.8 904,349 - MMJJ_RS04825 0.44 +0.2 904,390 + MMJJ_RS04825 0.47 +0.2 904,390 + MMJJ_RS04825 0.47 +0.3 904,393 - MMJJ_RS04825 0.47 -0.9 904,438 + MMJJ_RS04825 0.51 +0.5 904,482 - MMJJ_RS04825 0.54 -0.7 904,483 + MMJJ_RS04825 0.54 +0.2 904,503 - MMJJ_RS04825 0.56 -0.6 904,503 - MMJJ_RS04825 0.56 -2.1 904,504 + MMJJ_RS04825 0.56 -0.4 904,511 - MMJJ_RS04825 0.56 -0.5 904,512 + MMJJ_RS04825 0.56 +1.3 904,527 - MMJJ_RS04825 0.58 +0.3 904,527 - MMJJ_RS04825 0.58 +0.7 904,528 + MMJJ_RS04825 0.58 +0.3 904,528 + MMJJ_RS04825 0.58 +0.1 904,603 - MMJJ_RS04825 0.63 +0.0 904,604 + MMJJ_RS04825 0.64 +0.9 904,707 - MMJJ_RS04825 0.72 +0.3 904,725 - MMJJ_RS04825 0.73 +0.2 904,726 + MMJJ_RS04825 0.73 +0.2 904,752 - MMJJ_RS04825 0.75 -0.7 904,752 - MMJJ_RS04825 0.75 -0.2 904,753 + MMJJ_RS04825 0.75 +0.9
Or see this region's nucleotide sequence