Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS00405

Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS00390 and MMJJ_RS00395 are separated by 18 nucleotidesMMJJ_RS00395 and MMJJ_RS00400 are separated by 42 nucleotidesMMJJ_RS00400 and MMJJ_RS00405 are separated by 76 nucleotidesMMJJ_RS00405 and MMJJ_RS00410 are separated by 43 nucleotidesMMJJ_RS00410 and MMJJ_RS00415 are separated by 43 nucleotides MMJJ_RS00390: MMJJ_RS00390 - proteasome assembly chaperone family protein, at 73,947 to 74,654 _RS00390 MMJJ_RS00395: MMJJ_RS00395 - DUF473 domain-containing protein, at 74,673 to 75,050 _RS00395 MMJJ_RS00400: MMJJ_RS00400 - tautomerase family protein, at 75,093 to 75,287 _RS00400 MMJJ_RS00405: MMJJ_RS00405 - flavodoxin family protein, at 75,364 to 75,945 _RS00405 MMJJ_RS00410: MMJJ_RS00410 - hypothetical protein, at 75,989 to 76,417 _RS00410 MMJJ_RS00415: MMJJ_RS00415 - DedA family protein, at 76,461 to 76,943 _RS00415 Position (kb) 75 76Strain fitness (log2 ratio) -1 0 1 2 3at 74.385 kb on + strand, within MMJJ_RS00390at 74.385 kb on + strand, within MMJJ_RS00390at 74.408 kb on - strand, within MMJJ_RS00390at 74.409 kb on + strand, within MMJJ_RS00390at 74.421 kb on + strand, within MMJJ_RS00390at 74.470 kb on - strand, within MMJJ_RS00390at 74.496 kb on + strand, within MMJJ_RS00390at 74.501 kb on + strand, within MMJJ_RS00390at 74.512 kb on - strand, within MMJJ_RS00390at 74.642 kb on - strandat 74.642 kb on - strandat 74.642 kb on - strandat 74.643 kb on + strandat 74.646 kb on - strandat 74.646 kb on - strandat 74.646 kb on - strandat 74.646 kb on - strandat 74.646 kb on - strandat 74.646 kb on - strandat 74.646 kb on - strandat 74.646 kb on - strandat 74.646 kb on - strandat 74.646 kb on - strandat 74.646 kb on - strandat 74.646 kb on - strandat 74.647 kb on + strandat 74.647 kb on + strandat 74.647 kb on + strandat 74.647 kb on + strandat 74.647 kb on + strandat 74.647 kb on + strandat 74.647 kb on + strandat 74.647 kb on + strandat 74.647 kb on + strandat 74.647 kb on + strandat 74.647 kb on + strandat 74.648 kb on - strandat 74.649 kb on + strandat 74.653 kb on + strandat 74.653 kb on + strandat 74.653 kb on + strandat 74.765 kb on - strand, within MMJJ_RS00395at 74.876 kb on - strand, within MMJJ_RS00395at 74.882 kb on - strand, within MMJJ_RS00395at 74.882 kb on - strand, within MMJJ_RS00395at 74.883 kb on + strand, within MMJJ_RS00395at 74.923 kb on - strand, within MMJJ_RS00395at 74.923 kb on - strand, within MMJJ_RS00395at 74.923 kb on - strand, within MMJJ_RS00395at 74.924 kb on + strand, within MMJJ_RS00395at 74.932 kb on - strand, within MMJJ_RS00395at 74.960 kb on + strand, within MMJJ_RS00395at 75.016 kb on - strandat 75.038 kb on + strandat 75.039 kb on - strandat 75.109 kb on - strandat 75.124 kb on - strand, within MMJJ_RS00400at 75.181 kb on - strand, within MMJJ_RS00400at 75.211 kb on - strand, within MMJJ_RS00400at 75.212 kb on + strand, within MMJJ_RS00400at 75.215 kb on - strand, within MMJJ_RS00400at 75.215 kb on - strand, within MMJJ_RS00400at 75.266 kb on + strand, within MMJJ_RS00400at 75.266 kb on + strand, within MMJJ_RS00400at 75.272 kb on - strandat 75.300 kb on - strandat 75.301 kb on + strandat 75.342 kb on - strandat 75.343 kb on + strandat 75.371 kb on - strandat 75.371 kb on - strandat 75.434 kb on - strand, within MMJJ_RS00405at 75.472 kb on + strand, within MMJJ_RS00405at 75.494 kb on - strand, within MMJJ_RS00405at 75.540 kb on - strand, within MMJJ_RS00405at 75.540 kb on - strand, within MMJJ_RS00405at 75.541 kb on + strand, within MMJJ_RS00405at 75.541 kb on + strand, within MMJJ_RS00405at 75.541 kb on + strand, within MMJJ_RS00405at 75.548 kb on - strand, within MMJJ_RS00405at 75.596 kb on - strand, within MMJJ_RS00405at 75.596 kb on - strand, within MMJJ_RS00405at 75.616 kb on - strand, within MMJJ_RS00405at 75.616 kb on - strand, within MMJJ_RS00405at 75.685 kb on + strand, within MMJJ_RS00405at 75.754 kb on + strand, within MMJJ_RS00405at 75.781 kb on + strand, within MMJJ_RS00405at 75.827 kb on + strand, within MMJJ_RS00405at 75.941 kb on - strandat 75.942 kb on + strandat 75.943 kb on - strandat 75.946 kb on - strandat 75.947 kb on + strandat 75.966 kb on - strandat 75.976 kb on - strandat 76.026 kb on - strandat 76.030 kb on + strandat 76.077 kb on - strand, within MMJJ_RS00410at 76.082 kb on + strand, within MMJJ_RS00410at 76.085 kb on + strand, within MMJJ_RS00410at 76.086 kb on - strand, within MMJJ_RS00410at 76.086 kb on - strand, within MMJJ_RS00410at 76.086 kb on - strand, within MMJJ_RS00410at 76.086 kb on - strand, within MMJJ_RS00410at 76.102 kb on - strand, within MMJJ_RS00410at 76.152 kb on - strand, within MMJJ_RS00410at 76.198 kb on - strand, within MMJJ_RS00410at 76.198 kb on - strand, within MMJJ_RS00410at 76.251 kb on - strand, within MMJJ_RS00410at 76.252 kb on + strand, within MMJJ_RS00410at 76.268 kb on - strand, within MMJJ_RS00410at 76.268 kb on - strand, within MMJJ_RS00410at 76.269 kb on + strand, within MMJJ_RS00410at 76.269 kb on + strand, within MMJJ_RS00410at 76.269 kb on + strand, within MMJJ_RS00410at 76.269 kb on + strand, within MMJJ_RS00410at 76.292 kb on + strand, within MMJJ_RS00410at 76.292 kb on + strand, within MMJJ_RS00410at 76.292 kb on + strand, within MMJJ_RS00410at 76.299 kb on + strand, within MMJJ_RS00410at 76.299 kb on + strand, within MMJJ_RS00410at 76.416 kb on + strandat 76.416 kb on + strandat 76.416 kb on + strandat 76.424 kb on + strandat 76.458 kb on + strandat 76.458 kb on + strandat 76.463 kb on + strandat 76.463 kb on + strandat 76.482 kb on - strandat 76.483 kb on + strandat 76.513 kb on + strand, within MMJJ_RS00415at 76.519 kb on + strand, within MMJJ_RS00415at 76.582 kb on + strand, within MMJJ_RS00415at 76.647 kb on - strand, within MMJJ_RS00415at 76.647 kb on - strand, within MMJJ_RS00415at 76.680 kb on + strand, within MMJJ_RS00415at 76.751 kb on - strand, within MMJJ_RS00415at 76.751 kb on - strand, within MMJJ_RS00415at 76.763 kb on - strand, within MMJJ_RS00415at 76.783 kb on + strand, within MMJJ_RS00415at 76.833 kb on + strand, within MMJJ_RS00415at 76.833 kb on + strand, within MMJJ_RS00415at 76.836 kb on - strand, within MMJJ_RS00415at 76.837 kb on + strand, within MMJJ_RS00415at 76.837 kb on + strand, within MMJJ_RS00415at 76.838 kb on - strand, within MMJJ_RS00415at 76.839 kb on + strand, within MMJJ_RS00415at 76.839 kb on + strand, within MMJJ_RS00415at 76.839 kb on + strand, within MMJJ_RS00415at 76.845 kb on - strand, within MMJJ_RS00415at 76.903 kb on - strandat 76.908 kb on + strandat 76.908 kb on + strandat 76.911 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE
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74,385 + MMJJ_RS00390 0.62 -0.4
74,385 + MMJJ_RS00390 0.62 +1.0
74,408 - MMJJ_RS00390 0.65 +0.1
74,409 + MMJJ_RS00390 0.65 -0.2
74,421 + MMJJ_RS00390 0.67 -0.6
74,470 - MMJJ_RS00390 0.74 -1.1
74,496 + MMJJ_RS00390 0.78 -0.2
74,501 + MMJJ_RS00390 0.78 +0.5
74,512 - MMJJ_RS00390 0.80 -0.8
74,642 - +1.0
74,642 - -0.2
74,642 - -0.4
74,643 + +0.0
74,646 - -0.2
74,646 - -0.7
74,646 - +0.6
74,646 - -0.2
74,646 - -0.2
74,646 - -0.3
74,646 - -0.2
74,646 - +0.4
74,646 - -0.1
74,646 - -0.5
74,646 - +0.1
74,646 - +0.2
74,647 + -0.4
74,647 + +0.7
74,647 + -0.5
74,647 + -0.1
74,647 + -0.9
74,647 + +0.4
74,647 + -0.1
74,647 + +0.7
74,647 + -0.5
74,647 + +0.3
74,647 + +0.6
74,648 - -0.8
74,649 + +0.6
74,653 + +0.0
74,653 + +0.0
74,653 + +2.8
74,765 - MMJJ_RS00395 0.24 -0.0
74,876 - MMJJ_RS00395 0.54 +0.1
74,882 - MMJJ_RS00395 0.55 -0.0
74,882 - MMJJ_RS00395 0.55 -0.2
74,883 + MMJJ_RS00395 0.56 -1.6
74,923 - MMJJ_RS00395 0.66 -0.8
74,923 - MMJJ_RS00395 0.66 -0.7
74,923 - MMJJ_RS00395 0.66 +0.1
74,924 + MMJJ_RS00395 0.66 -0.9
74,932 - MMJJ_RS00395 0.69 -0.5
74,960 + MMJJ_RS00395 0.76 -0.2
75,016 - -0.0
75,038 + -0.3
75,039 - +0.6
75,109 - +0.2
75,124 - MMJJ_RS00400 0.16 +0.2
75,181 - MMJJ_RS00400 0.45 +0.5
75,211 - MMJJ_RS00400 0.61 +0.3
75,212 + MMJJ_RS00400 0.61 -0.6
75,215 - MMJJ_RS00400 0.63 +0.5
75,215 - MMJJ_RS00400 0.63 -0.4
75,266 + MMJJ_RS00400 0.89 -0.3
75,266 + MMJJ_RS00400 0.89 +0.1
75,272 - -0.7
75,300 - -0.0
75,301 + -0.5
75,342 - +0.3
75,343 + +0.5
75,371 - +0.5
75,371 - +0.9
75,434 - MMJJ_RS00405 0.12 -0.2
75,472 + MMJJ_RS00405 0.19 +0.2
75,494 - MMJJ_RS00405 0.22 +0.7
75,540 - MMJJ_RS00405 0.30 -0.3
75,540 - MMJJ_RS00405 0.30 +0.1
75,541 + MMJJ_RS00405 0.30 +0.2
75,541 + MMJJ_RS00405 0.30 +0.6
75,541 + MMJJ_RS00405 0.30 -0.4
75,548 - MMJJ_RS00405 0.32 +0.6
75,596 - MMJJ_RS00405 0.40 -0.3
75,596 - MMJJ_RS00405 0.40 +0.3
75,616 - MMJJ_RS00405 0.43 +0.4
75,616 - MMJJ_RS00405 0.43 +0.5
75,685 + MMJJ_RS00405 0.55 -0.9
75,754 + MMJJ_RS00405 0.67 +0.2
75,781 + MMJJ_RS00405 0.72 +0.0
75,827 + MMJJ_RS00405 0.80 +0.0
75,941 - -0.4
75,942 + +0.2
75,943 - +0.1
75,946 - -0.1
75,947 + -0.4
75,966 - -0.8
75,976 - +0.7
76,026 - -0.6
76,030 + -0.3
76,077 - MMJJ_RS00410 0.21 -0.1
76,082 + MMJJ_RS00410 0.22 -0.2
76,085 + MMJJ_RS00410 0.22 +0.6
76,086 - MMJJ_RS00410 0.23 +0.4
76,086 - MMJJ_RS00410 0.23 -0.0
76,086 - MMJJ_RS00410 0.23 -0.9
76,086 - MMJJ_RS00410 0.23 +0.7
76,102 - MMJJ_RS00410 0.26 -0.8
76,152 - MMJJ_RS00410 0.38 -0.6
76,198 - MMJJ_RS00410 0.49 +0.8
76,198 - MMJJ_RS00410 0.49 -0.4
76,251 - MMJJ_RS00410 0.61 -0.8
76,252 + MMJJ_RS00410 0.61 +0.4
76,268 - MMJJ_RS00410 0.65 +0.6
76,268 - MMJJ_RS00410 0.65 +0.5
76,269 + MMJJ_RS00410 0.65 -0.9
76,269 + MMJJ_RS00410 0.65 -0.2
76,269 + MMJJ_RS00410 0.65 +0.4
76,269 + MMJJ_RS00410 0.65 -1.2
76,292 + MMJJ_RS00410 0.71 +0.9
76,292 + MMJJ_RS00410 0.71 +0.1
76,292 + MMJJ_RS00410 0.71 +0.3
76,299 + MMJJ_RS00410 0.72 -0.1
76,299 + MMJJ_RS00410 0.72 -0.6
76,416 + +0.0
76,416 + +0.4
76,416 + +0.1
76,424 + +1.0
76,458 + +0.4
76,458 + +1.0
76,463 + +0.6
76,463 + -0.8
76,482 - -0.2
76,483 + -0.4
76,513 + MMJJ_RS00415 0.11 +0.3
76,519 + MMJJ_RS00415 0.12 +1.0
76,582 + MMJJ_RS00415 0.25 +1.0
76,647 - MMJJ_RS00415 0.39 +0.4
76,647 - MMJJ_RS00415 0.39 +0.4
76,680 + MMJJ_RS00415 0.45 -0.2
76,751 - MMJJ_RS00415 0.60 +1.2
76,751 - MMJJ_RS00415 0.60 +0.5
76,763 - MMJJ_RS00415 0.63 +0.4
76,783 + MMJJ_RS00415 0.67 -0.1
76,833 + MMJJ_RS00415 0.77 -0.4
76,833 + MMJJ_RS00415 0.77 -1.5
76,836 - MMJJ_RS00415 0.78 +0.3
76,837 + MMJJ_RS00415 0.78 +1.5
76,837 + MMJJ_RS00415 0.78 -1.0
76,838 - MMJJ_RS00415 0.78 +0.4
76,839 + MMJJ_RS00415 0.78 +0.8
76,839 + MMJJ_RS00415 0.78 -0.0
76,839 + MMJJ_RS00415 0.78 +1.6
76,845 - MMJJ_RS00415 0.80 +0.5
76,903 - -0.5
76,908 + -1.6
76,908 + +0.7
76,911 - -0.0

Or see this region's nucleotide sequence