Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS00315

Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE
58,455	-		MMJJ_RS00305	0.85	+0.4
58,460	-		MMJJ_RS00305	0.86	+0.3
58,464	-		MMJJ_RS00305	0.87	-1.1
58,464	-		MMJJ_RS00305	0.87	-0.0
58,465	+		MMJJ_RS00305	0.87	+0.2
58,468	+		MMJJ_RS00305	0.87	+0.4
58,508	-				-0.6
58,508	-				-0.7
58,508	-				+0.1
58,509	+				-0.6
58,509	+				-0.3
58,542	+				-0.2
58,574	+				+0.4
58,600	-				-0.9
58,600	-				-0.6
58,600	-				+1.2
58,601	+				+0.4
58,625	+				+0.1
58,628	-				+0.3
58,628	-				-0.1
58,629	+				-0.4
58,629	+				-0.2
58,671	-				+0.0
58,671	-				-0.3
58,672	+				-0.5
58,685	-				+0.1
58,694	+				-0.7
58,694	+				-0.4
58,697	-				-0.2
58,698	+		MMJJ_RS00310	0.10	+0.6
58,698	+		MMJJ_RS00310	0.10	-0.2
58,705	+		MMJJ_RS00310	0.11	-0.6
58,705	+		MMJJ_RS00310	0.11	+0.3
58,720	-		MMJJ_RS00310	0.13	+0.0
58,750	-		MMJJ_RS00310	0.16	+0.6
58,758	+		MMJJ_RS00310	0.17	+0.6
58,771	+		MMJJ_RS00310	0.19	+0.5
58,807	-		MMJJ_RS00310	0.23	+0.4
58,818	-		MMJJ_RS00310	0.25	-0.2
58,818	-		MMJJ_RS00310	0.25	+0.8
58,824	-		MMJJ_RS00310	0.26	-0.1
58,825	+		MMJJ_RS00310	0.26	+0.4
58,853	-		MMJJ_RS00310	0.29	+0.4
58,856	+		MMJJ_RS00310	0.30	+0.3
58,887	-		MMJJ_RS00310	0.33	-0.3
58,888	+		MMJJ_RS00310	0.33	-0.0
58,971	-		MMJJ_RS00310	0.44	-1.0
59,013	-		MMJJ_RS00310	0.49	-0.8
59,047	+		MMJJ_RS00310	0.53	-0.5
59,050	+		MMJJ_RS00310	0.53	-0.1
59,050	+		MMJJ_RS00310	0.53	+0.2
59,050	+		MMJJ_RS00310	0.53	+0.7
59,050	+		MMJJ_RS00310	0.53	-1.2
59,072	-		MMJJ_RS00310	0.56	-0.6
59,072	-		MMJJ_RS00310	0.56	+0.6
59,111	+		MMJJ_RS00310	0.61	+0.5
59,120	-		MMJJ_RS00310	0.62	-1.3
59,133	-		MMJJ_RS00310	0.64	-1.0
59,134	+		MMJJ_RS00310	0.64	-1.3
59,141	+		MMJJ_RS00310	0.65	-1.9
59,167	+		MMJJ_RS00310	0.68	+0.4
59,253	-		MMJJ_RS00310	0.78	+0.2
59,311	+		MMJJ_RS00310	0.85	+0.9
59,377	-				-0.2
59,378	+				+0.4
59,422	+				+0.1
59,471	+				+0.1
59,474	-				+0.0
59,474	-				+0.0
59,474	-				+0.6
59,475	+				-0.6
59,717	-		MMJJ_RS00315	0.19	-0.4
59,792	-		MMJJ_RS00315	0.24	-1.1
59,846	+		MMJJ_RS00315	0.28	+1.0
59,884	-		MMJJ_RS00315	0.31	-0.5
59,990	+		MMJJ_RS00315	0.38	+0.4
60,076	-		MMJJ_RS00315	0.44	-0.3
60,154	-		MMJJ_RS00315	0.50	+0.5
60,203	+		MMJJ_RS00315	0.53	+0.5
60,222	+		MMJJ_RS00315	0.55	+0.2
60,311	-		MMJJ_RS00315	0.61	+0.0
60,312	+		MMJJ_RS00315	0.61	-0.7
60,312	+		MMJJ_RS00315	0.61	-0.5
60,347	-		MMJJ_RS00315	0.64	+0.1
60,367	-		MMJJ_RS00315	0.65	+0.3
60,395	-		MMJJ_RS00315	0.67	-0.5
60,396	+		MMJJ_RS00315	0.67	-0.4
60,398	-		MMJJ_RS00315	0.67	-0.3
60,399	+		MMJJ_RS00315	0.67	+0.2
60,416	-		MMJJ_RS00315	0.69	-1.6
60,417	+		MMJJ_RS00315	0.69	+0.3
60,417	+		MMJJ_RS00315	0.69	-0.0
60,417	+		MMJJ_RS00315	0.69	+1.2
60,430	-		MMJJ_RS00315	0.70	+0.7
60,430	-		MMJJ_RS00315	0.70	+1.7
60,432	-		MMJJ_RS00315	0.70	-0.6
60,432	-		MMJJ_RS00315	0.70	-0.4
60,433	+		MMJJ_RS00315	0.70	-0.4
60,433	+		MMJJ_RS00315	0.70	-0.8
60,433	+		MMJJ_RS00315	0.70	+0.1
60,439	-		MMJJ_RS00315	0.70	+0.4
60,476	-		MMJJ_RS00315	0.73	+0.2
60,488	-		MMJJ_RS00315	0.74	+1.0
60,574	-		MMJJ_RS00315	0.80	+0.4
60,630	+		MMJJ_RS00315	0.84	+0.2
60,659	-		MMJJ_RS00315	0.86	+0.3
60,673	+		MMJJ_RS00315	0.87	-0.2
60,689	-		MMJJ_RS00315	0.88	+0.2
60,689	-		MMJJ_RS00315	0.88	+0.5
60,689	-		MMJJ_RS00315	0.88	+0.2
60,689	-		MMJJ_RS00315	0.88	+0.9
60,690	+		MMJJ_RS00315	0.88	+1.2
60,690	+		MMJJ_RS00315	0.88	+0.4
60,690	+		MMJJ_RS00315	0.88	-0.0
60,707	+		MMJJ_RS00315	0.89	-0.2
60,727	-				+0.2
60,774	+				+0.1
60,774	+				+0.5
60,774	+				+0.9
60,774	+				-0.5
60,824	-				+0.4
60,825	+				+0.1
60,856	-				+0.8
60,860	-				+0.5
60,860	-				+0.4
60,860	-				-0.8
60,861	+				+1.0
60,861	+				+0.6
60,868	-				-0.2
60,868	-				+0.1
60,870	-				-0.3
60,870	-				+1.3
60,870	-				-1.0
60,871	+				+0.0
60,874	-				+0.2
60,874	-				-0.6
60,874	-				+0.9
60,877	+				+0.2
60,877	+				-0.3
60,879	+				+1.5
60,879	+				-0.3
60,879	+				+0.0
60,879	+				-0.3
60,903	+				-0.1
60,923	-		MMJJ_RS00320	0.11	+1.4
60,931	+		MMJJ_RS00320	0.12	-0.6
60,936	-		MMJJ_RS00320	0.14	-0.6
60,938	-		MMJJ_RS00320	0.14	-0.3
60,939	+		MMJJ_RS00320	0.14	+0.4
60,952	-		MMJJ_RS00320	0.17	-0.1
60,953	+		MMJJ_RS00320	0.17	-0.5
60,981	-		MMJJ_RS00320	0.23	-0.4
61,039	+		MMJJ_RS00320	0.36	-0.0
61,043	-		MMJJ_RS00320	0.37	+0.1
61,207	+		MMJJ_RS00320	0.72	-0.5
61,243	-		MMJJ_RS00320	0.80	+0.1
61,244	+		MMJJ_RS00320	0.80	+0.0
61,396	-				+1.2
61,397	+				+0.1
61,402	+				-0.5
61,456	-				-0.2
61,456	-				+0.4
61,456	-				-0.4
61,522	-				-0.2
61,523	+				+0.0
61,523	+				-0.6
61,529	-				+0.3
61,529	-				+0.6
61,530	+				+0.2
61,561	+				-0.4
61,596	-				+0.4
61,662	-		MMJJ_RS00325	0.11	-0.0
61,662	-		MMJJ_RS00325	0.11	-0.0
61,662	-		MMJJ_RS00325	0.11	+0.3
61,663	+		MMJJ_RS00325	0.11	-0.1
61,694	-		MMJJ_RS00325	0.13	+0.8
61,694	-		MMJJ_RS00325	0.13	-0.5
61,695	+		MMJJ_RS00325	0.13	-0.8
61,695	+		MMJJ_RS00325	0.13	+0.3
61,698	-		MMJJ_RS00325	0.13	+0.1
61,699	+		MMJJ_RS00325	0.13	-0.5
61,709	+		MMJJ_RS00325	0.13	-0.2
61,718	+		MMJJ_RS00325	0.13	+0.2
61,729	-		MMJJ_RS00325	0.14	+0.2
61,730	+		MMJJ_RS00325	0.14	+0.2
61,763	+		MMJJ_RS00325	0.15	-0.0
61,797	-		MMJJ_RS00325	0.16	+0.4