Experiment: R2A with Chloramphenicol 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS02285 and CA265_RS02290 are separated by 84 nucleotides CA265_RS02290 and CA265_RS02295 overlap by 25 nucleotides CA265_RS02295 and CA265_RS02300 are separated by 6 nucleotides CA265_RS02300 and CA265_RS02305 are separated by 92 nucleotides
CA265_RS02285: CA265_RS02285 - hypothetical protein, at 572,294 to 573,037
_RS02285
CA265_RS02290: CA265_RS02290 - alpha-ketoglutarate-dependent dioxygenase AlkB, at 573,122 to 573,730
_RS02290
CA265_RS02295: CA265_RS02295 - metal-binding protein, at 573,706 to 573,954
_RS02295
CA265_RS02300: CA265_RS02300 - cysteine methyltransferase, at 573,961 to 574,800
_RS02300
CA265_RS02305: CA265_RS02305 - hypothetical protein, at 574,893 to 575,429
_RS02305
Position (kb) 573
574 Strain fitness (log2 ratio) -1
0
1
2 at 572.727 kb on + strand, within CA265_RS02285 at 572.739 kb on - strand, within CA265_RS02285 at 572.739 kb on - strand, within CA265_RS02285 at 572.849 kb on + strand, within CA265_RS02285 at 572.866 kb on + strand, within CA265_RS02285 at 572.879 kb on - strand, within CA265_RS02285 at 572.906 kb on - strand, within CA265_RS02285 at 572.973 kb on - strand at 572.973 kb on - strand at 573.058 kb on - strand at 573.063 kb on + strand at 573.063 kb on + strand at 573.084 kb on - strand at 573.128 kb on + strand at 573.128 kb on + strand at 573.139 kb on + strand at 573.164 kb on - strand at 573.251 kb on + strand, within CA265_RS02290 at 573.394 kb on - strand, within CA265_RS02290 at 573.400 kb on + strand, within CA265_RS02290 at 573.425 kb on - strand, within CA265_RS02290 at 573.496 kb on + strand, within CA265_RS02290 at 573.504 kb on + strand, within CA265_RS02290 at 573.510 kb on + strand, within CA265_RS02290 at 573.511 kb on - strand, within CA265_RS02290 at 573.539 kb on + strand, within CA265_RS02290 at 573.540 kb on - strand, within CA265_RS02290 at 573.556 kb on + strand, within CA265_RS02290 at 573.571 kb on + strand, within CA265_RS02290 at 573.605 kb on - strand, within CA265_RS02290 at 573.708 kb on - strand at 573.737 kb on + strand, within CA265_RS02295 at 573.738 kb on - strand, within CA265_RS02295 at 573.738 kb on - strand, within CA265_RS02295 at 573.738 kb on - strand, within CA265_RS02295 at 573.784 kb on + strand, within CA265_RS02295 at 573.850 kb on - strand, within CA265_RS02295 at 573.850 kb on - strand, within CA265_RS02295 at 574.051 kb on + strand, within CA265_RS02300 at 574.052 kb on - strand, within CA265_RS02300 at 574.052 kb on - strand, within CA265_RS02300 at 574.108 kb on + strand, within CA265_RS02300 at 574.126 kb on - strand, within CA265_RS02300 at 574.131 kb on + strand, within CA265_RS02300 at 574.157 kb on + strand, within CA265_RS02300 at 574.181 kb on - strand, within CA265_RS02300 at 574.181 kb on - strand, within CA265_RS02300 at 574.228 kb on + strand, within CA265_RS02300 at 574.240 kb on - strand, within CA265_RS02300 at 574.277 kb on + strand, within CA265_RS02300 at 574.278 kb on - strand, within CA265_RS02300 at 574.299 kb on + strand, within CA265_RS02300 at 574.306 kb on + strand, within CA265_RS02300 at 574.307 kb on - strand, within CA265_RS02300 at 574.605 kb on - strand, within CA265_RS02300 at 574.614 kb on + strand, within CA265_RS02300 at 574.619 kb on + strand, within CA265_RS02300 at 574.619 kb on + strand, within CA265_RS02300 at 574.641 kb on + strand, within CA265_RS02300 at 574.687 kb on + strand, within CA265_RS02300 at 574.714 kb on + strand, within CA265_RS02300 at 574.811 kb on - strand at 574.816 kb on - strand at 574.898 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloramphenicol 0.001 mg/ml remove 572,727 + CA265_RS02285 0.58 +0.7 572,739 - CA265_RS02285 0.60 -0.7 572,739 - CA265_RS02285 0.60 +0.7 572,849 + CA265_RS02285 0.75 -0.1 572,866 + CA265_RS02285 0.77 +0.7 572,879 - CA265_RS02285 0.79 +1.2 572,906 - CA265_RS02285 0.82 -0.1 572,973 - +0.6 572,973 - -0.1 573,058 - +0.3 573,063 + +0.1 573,063 + -1.1 573,084 - -0.1 573,128 + +0.3 573,128 + -0.2 573,139 + -0.2 573,164 - -0.3 573,251 + CA265_RS02290 0.21 -0.1 573,394 - CA265_RS02290 0.45 +0.5 573,400 + CA265_RS02290 0.46 +0.5 573,425 - CA265_RS02290 0.50 -0.4 573,496 + CA265_RS02290 0.61 +2.1 573,504 + CA265_RS02290 0.63 +0.6 573,510 + CA265_RS02290 0.64 +0.4 573,511 - CA265_RS02290 0.64 -0.9 573,539 + CA265_RS02290 0.68 -0.7 573,540 - CA265_RS02290 0.69 -0.2 573,556 + CA265_RS02290 0.71 -0.6 573,571 + CA265_RS02290 0.74 -0.3 573,605 - CA265_RS02290 0.79 -0.3 573,708 - -0.3 573,737 + CA265_RS02295 0.12 -0.9 573,738 - CA265_RS02295 0.13 -0.6 573,738 - CA265_RS02295 0.13 +0.2 573,738 - CA265_RS02295 0.13 +0.4 573,784 + CA265_RS02295 0.31 -0.4 573,850 - CA265_RS02295 0.58 -1.3 573,850 - CA265_RS02295 0.58 -0.5 574,051 + CA265_RS02300 0.11 -0.2 574,052 - CA265_RS02300 0.11 -1.1 574,052 - CA265_RS02300 0.11 +1.2 574,108 + CA265_RS02300 0.17 -0.4 574,126 - CA265_RS02300 0.20 -0.6 574,131 + CA265_RS02300 0.20 -0.6 574,157 + CA265_RS02300 0.23 +0.5 574,181 - CA265_RS02300 0.26 -1.1 574,181 - CA265_RS02300 0.26 +0.8 574,228 + CA265_RS02300 0.32 -0.1 574,240 - CA265_RS02300 0.33 +0.7 574,277 + CA265_RS02300 0.38 -0.6 574,278 - CA265_RS02300 0.38 +0.7 574,299 + CA265_RS02300 0.40 +0.5 574,306 + CA265_RS02300 0.41 -0.3 574,307 - CA265_RS02300 0.41 -0.2 574,605 - CA265_RS02300 0.77 +0.5 574,614 + CA265_RS02300 0.78 -0.3 574,619 + CA265_RS02300 0.78 +0.7 574,619 + CA265_RS02300 0.78 +0.2 574,641 + CA265_RS02300 0.81 +0.1 574,687 + CA265_RS02300 0.86 +1.7 574,714 + CA265_RS02300 0.90 +0.1 574,811 - -1.3 574,816 - +0.4 574,898 + -0.4
Or see this region's nucleotide sequence