Experiment: R2A with Chloramphenicol 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS00670 and CA265_RS00675 are separated by 18 nucleotides CA265_RS00675 and CA265_RS00680 are separated by 25 nucleotides CA265_RS00680 and CA265_RS00685 are separated by 371 nucleotides CA265_RS00685 and CA265_RS00690 are separated by 65 nucleotides
CA265_RS00670: CA265_RS00670 - metallophosphoesterase, at 152,886 to 153,728
_RS00670
CA265_RS00675: CA265_RS00675 - hypothetical protein, at 153,747 to 154,586
_RS00675
CA265_RS00680: CA265_RS00680 - hypothetical protein, at 154,612 to 157,053
_RS00680
CA265_RS00685: CA265_RS00685 - peptide-methionine (S)-S-oxide reductase, at 157,425 to 157,913
_RS00685
CA265_RS00690: CA265_RS00690 - hypothetical protein, at 157,979 to 158,374
_RS00690
Position (kb)
154
155
156
157
158 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 153.627 kb on + strand, within CA265_RS00670 at 153.678 kb on + strand at 153.743 kb on + strand at 153.758 kb on - strand at 153.782 kb on + strand at 153.927 kb on + strand, within CA265_RS00675 at 154.112 kb on - strand, within CA265_RS00675 at 154.127 kb on - strand, within CA265_RS00675 at 154.144 kb on + strand, within CA265_RS00675 at 154.144 kb on + strand, within CA265_RS00675 at 154.187 kb on + strand, within CA265_RS00675 at 154.311 kb on - strand, within CA265_RS00675 at 154.320 kb on + strand, within CA265_RS00675 at 154.321 kb on - strand, within CA265_RS00675 at 154.339 kb on + strand, within CA265_RS00675 at 154.339 kb on + strand, within CA265_RS00675 at 154.340 kb on - strand, within CA265_RS00675 at 154.352 kb on - strand, within CA265_RS00675 at 154.397 kb on - strand, within CA265_RS00675 at 154.547 kb on + strand at 154.548 kb on - strand at 154.628 kb on + strand at 154.631 kb on + strand at 154.802 kb on - strand at 154.830 kb on + strand at 154.918 kb on - strand, within CA265_RS00680 at 154.920 kb on + strand, within CA265_RS00680 at 154.920 kb on + strand, within CA265_RS00680 at 154.940 kb on - strand, within CA265_RS00680 at 154.960 kb on - strand, within CA265_RS00680 at 154.980 kb on + strand, within CA265_RS00680 at 155.090 kb on + strand, within CA265_RS00680 at 155.090 kb on + strand, within CA265_RS00680 at 155.091 kb on - strand, within CA265_RS00680 at 155.093 kb on - strand, within CA265_RS00680 at 155.093 kb on - strand, within CA265_RS00680 at 155.113 kb on - strand, within CA265_RS00680 at 155.123 kb on - strand, within CA265_RS00680 at 155.218 kb on + strand, within CA265_RS00680 at 155.315 kb on + strand, within CA265_RS00680 at 155.316 kb on - strand, within CA265_RS00680 at 155.316 kb on - strand, within CA265_RS00680 at 155.326 kb on - strand, within CA265_RS00680 at 155.398 kb on - strand, within CA265_RS00680 at 155.419 kb on - strand, within CA265_RS00680 at 155.428 kb on + strand, within CA265_RS00680 at 155.471 kb on + strand, within CA265_RS00680 at 155.495 kb on - strand, within CA265_RS00680 at 155.514 kb on + strand, within CA265_RS00680 at 155.538 kb on - strand, within CA265_RS00680 at 155.694 kb on + strand, within CA265_RS00680 at 155.695 kb on - strand, within CA265_RS00680 at 155.763 kb on + strand, within CA265_RS00680 at 155.808 kb on - strand, within CA265_RS00680 at 155.810 kb on - strand, within CA265_RS00680 at 155.811 kb on + strand, within CA265_RS00680 at 155.841 kb on + strand, within CA265_RS00680 at 155.935 kb on - strand, within CA265_RS00680 at 156.027 kb on + strand, within CA265_RS00680 at 156.058 kb on + strand, within CA265_RS00680 at 156.095 kb on + strand, within CA265_RS00680 at 156.144 kb on - strand, within CA265_RS00680 at 156.144 kb on - strand, within CA265_RS00680 at 156.170 kb on - strand, within CA265_RS00680 at 156.312 kb on + strand, within CA265_RS00680 at 156.313 kb on - strand, within CA265_RS00680 at 156.333 kb on + strand, within CA265_RS00680 at 156.333 kb on + strand, within CA265_RS00680 at 156.334 kb on - strand, within CA265_RS00680 at 156.462 kb on + strand, within CA265_RS00680 at 156.462 kb on + strand, within CA265_RS00680 at 156.493 kb on - strand, within CA265_RS00680 at 156.670 kb on + strand, within CA265_RS00680 at 156.703 kb on + strand, within CA265_RS00680 at 156.704 kb on - strand, within CA265_RS00680 at 156.708 kb on + strand, within CA265_RS00680 at 156.708 kb on + strand, within CA265_RS00680 at 156.744 kb on - strand, within CA265_RS00680 at 156.788 kb on + strand, within CA265_RS00680 at 156.788 kb on + strand, within CA265_RS00680 at 156.791 kb on - strand, within CA265_RS00680 at 156.791 kb on - strand, within CA265_RS00680 at 156.826 kb on + strand at 156.827 kb on - strand at 157.068 kb on + strand at 157.174 kb on + strand at 157.174 kb on + strand at 157.201 kb on + strand at 157.202 kb on - strand at 157.248 kb on - strand at 157.379 kb on + strand at 157.410 kb on + strand at 157.460 kb on + strand at 157.460 kb on + strand at 157.461 kb on - strand at 157.461 kb on - strand at 157.620 kb on - strand, within CA265_RS00685 at 157.625 kb on - strand, within CA265_RS00685 at 157.649 kb on + strand, within CA265_RS00685 at 157.650 kb on - strand, within CA265_RS00685 at 157.654 kb on - strand, within CA265_RS00685 at 157.705 kb on + strand, within CA265_RS00685 at 157.706 kb on - strand, within CA265_RS00685 at 157.706 kb on - strand, within CA265_RS00685 at 157.728 kb on + strand, within CA265_RS00685 at 157.817 kb on - strand, within CA265_RS00685 at 157.914 kb on - strand at 157.915 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloramphenicol 0.001 mg/ml remove 153,627 + CA265_RS00670 0.88 -1.1 153,678 + +0.8 153,743 + -0.8 153,758 - +0.3 153,782 + -0.3 153,927 + CA265_RS00675 0.21 +0.7 154,112 - CA265_RS00675 0.43 -0.9 154,127 - CA265_RS00675 0.45 -0.3 154,144 + CA265_RS00675 0.47 -0.8 154,144 + CA265_RS00675 0.47 -0.6 154,187 + CA265_RS00675 0.52 -0.2 154,311 - CA265_RS00675 0.67 -0.4 154,320 + CA265_RS00675 0.68 -0.1 154,321 - CA265_RS00675 0.68 +0.1 154,339 + CA265_RS00675 0.70 -0.9 154,339 + CA265_RS00675 0.70 -0.4 154,340 - CA265_RS00675 0.71 -0.5 154,352 - CA265_RS00675 0.72 +1.1 154,397 - CA265_RS00675 0.77 +0.4 154,547 + -0.8 154,548 - +0.9 154,628 + +0.1 154,631 + -1.5 154,802 - -0.3 154,830 + +0.4 154,918 - CA265_RS00680 0.13 -0.1 154,920 + CA265_RS00680 0.13 +0.6 154,920 + CA265_RS00680 0.13 +0.8 154,940 - CA265_RS00680 0.13 -0.9 154,960 - CA265_RS00680 0.14 -0.8 154,980 + CA265_RS00680 0.15 -0.2 155,090 + CA265_RS00680 0.20 +0.7 155,090 + CA265_RS00680 0.20 -1.2 155,091 - CA265_RS00680 0.20 +0.4 155,093 - CA265_RS00680 0.20 -0.2 155,093 - CA265_RS00680 0.20 -0.1 155,113 - CA265_RS00680 0.21 -0.1 155,123 - CA265_RS00680 0.21 -0.1 155,218 + CA265_RS00680 0.25 -0.7 155,315 + CA265_RS00680 0.29 +0.1 155,316 - CA265_RS00680 0.29 +1.6 155,316 - CA265_RS00680 0.29 -1.0 155,326 - CA265_RS00680 0.29 +0.1 155,398 - CA265_RS00680 0.32 -2.3 155,419 - CA265_RS00680 0.33 -0.3 155,428 + CA265_RS00680 0.33 -0.8 155,471 + CA265_RS00680 0.35 -0.1 155,495 - CA265_RS00680 0.36 -0.5 155,514 + CA265_RS00680 0.37 -0.6 155,538 - CA265_RS00680 0.38 -0.4 155,694 + CA265_RS00680 0.44 -2.1 155,695 - CA265_RS00680 0.44 -0.5 155,763 + CA265_RS00680 0.47 -1.3 155,808 - CA265_RS00680 0.49 -1.7 155,810 - CA265_RS00680 0.49 -0.6 155,811 + CA265_RS00680 0.49 -0.7 155,841 + CA265_RS00680 0.50 +0.1 155,935 - CA265_RS00680 0.54 +0.9 156,027 + CA265_RS00680 0.58 -0.8 156,058 + CA265_RS00680 0.59 -0.7 156,095 + CA265_RS00680 0.61 -1.3 156,144 - CA265_RS00680 0.63 -0.2 156,144 - CA265_RS00680 0.63 -0.2 156,170 - CA265_RS00680 0.64 -0.0 156,312 + CA265_RS00680 0.70 -1.2 156,313 - CA265_RS00680 0.70 +1.0 156,333 + CA265_RS00680 0.70 +0.1 156,333 + CA265_RS00680 0.70 -0.6 156,334 - CA265_RS00680 0.71 -1.0 156,462 + CA265_RS00680 0.76 -0.9 156,462 + CA265_RS00680 0.76 -0.7 156,493 - CA265_RS00680 0.77 -0.3 156,670 + CA265_RS00680 0.84 +0.9 156,703 + CA265_RS00680 0.86 -0.4 156,704 - CA265_RS00680 0.86 -0.7 156,708 + CA265_RS00680 0.86 +0.9 156,708 + CA265_RS00680 0.86 -0.6 156,744 - CA265_RS00680 0.87 +0.7 156,788 + CA265_RS00680 0.89 -1.5 156,788 + CA265_RS00680 0.89 -2.5 156,791 - CA265_RS00680 0.89 -1.1 156,791 - CA265_RS00680 0.89 +1.4 156,826 + -0.4 156,827 - +0.1 157,068 + +0.3 157,174 + +1.2 157,174 + +0.0 157,201 + -0.2 157,202 - -0.7 157,248 - +0.1 157,379 + +0.8 157,410 + -0.3 157,460 + -0.1 157,460 + +0.3 157,461 - -0.2 157,461 - -0.8 157,620 - CA265_RS00685 0.40 +0.3 157,625 - CA265_RS00685 0.41 -0.1 157,649 + CA265_RS00685 0.46 +0.2 157,650 - CA265_RS00685 0.46 +0.5 157,654 - CA265_RS00685 0.47 -0.1 157,705 + CA265_RS00685 0.57 -0.7 157,706 - CA265_RS00685 0.57 -0.4 157,706 - CA265_RS00685 0.57 -0.1 157,728 + CA265_RS00685 0.62 -0.2 157,817 - CA265_RS00685 0.80 +3.2 157,914 - +0.1 157,915 + -1.0
Or see this region's nucleotide sequence