Experiment: MoYLS4 with Sisomicin 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2723 and DvMF_2724 overlap by 11 nucleotides DvMF_2724 and DvMF_2725 are separated by 355 nucleotides DvMF_2725 and DvMF_2726 are separated by 853 nucleotides
DvMF_2723: DvMF_2723 - Radical SAM domain protein (RefSeq), at 3,446,611 to 3,447,765
_2723
DvMF_2724: DvMF_2724 - hypothetical protein (RefSeq), at 3,447,755 to 3,448,021
_2724
DvMF_2725: DvMF_2725 - Resolvase domain protein (RefSeq), at 3,448,377 to 3,449,063
_2725
DvMF_2726: DvMF_2726 - ParB domain protein nuclease (RefSeq), at 3,449,917 to 3,450,681
_2726
Position (kb)
3448
3449
3450 Strain fitness (log2 ratio)
-1
0
1 at 3448.083 kb on + strand at 3448.211 kb on - strand at 3448.228 kb on + strand at 3448.388 kb on - strand at 3448.509 kb on - strand, within DvMF_2725 at 3448.593 kb on + strand, within DvMF_2725 at 3449.012 kb on + strand at 3449.192 kb on + strand at 3449.300 kb on - strand at 3449.406 kb on + strand at 3449.708 kb on - strand at 3449.748 kb on + strand at 3449.766 kb on + strand at 3449.811 kb on - strand at 3449.813 kb on + strand at 3449.815 kb on + strand at 3449.815 kb on + strand at 3449.823 kb on - strand at 3449.839 kb on + strand at 3449.839 kb on + strand at 3449.839 kb on + strand at 3449.839 kb on + strand at 3449.839 kb on + strand at 3449.839 kb on + strand at 3449.847 kb on - strand at 3449.894 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Sisomicin 0.03125 mg/ml remove 3,448,083 + -1.6 3,448,211 - -1.5 3,448,228 + -0.2 3,448,388 - -0.3 3,448,509 - DvMF_2725 0.19 -0.3 3,448,593 + DvMF_2725 0.31 +0.8 3,449,012 + +1.6 3,449,192 + -0.5 3,449,300 - +0.2 3,449,406 + -0.8 3,449,708 - -0.4 3,449,748 + -0.3 3,449,766 + -0.0 3,449,811 - +0.0 3,449,813 + -1.0 3,449,815 + -0.1 3,449,815 + -0.1 3,449,823 - -1.5 3,449,839 + -0.4 3,449,839 + -0.8 3,449,839 + +0.1 3,449,839 + -0.4 3,449,839 + +0.2 3,449,839 + -0.4 3,449,847 - -0.2 3,449,894 + -0.2
Or see this region's nucleotide sequence