Experiment: MoYLS4 with Sisomicin 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2458 and DvMF_2459 are separated by 13 nucleotides DvMF_2459 and frdB are separated by 150 nucleotides
DvMF_2458: DvMF_2458 - fumarate reductase respiratory complex transmembrane subunit (RefSeq), at 3,100,909 to 3,101,562
_2458
DvMF_2459: DvMF_2459 - fumarate reductase flavoprotein subunit (RefSeq), at 3,101,576 to 3,103,393
_2459
DvMF_2460: frdB - fumarate reductase iron-sulfur subunit (RefSeq), at 3,103,544 to 3,104,287
frdB
Position (kb)
3101
3102
3103
3104 Strain fitness (log2 ratio)
-1
0
1 at 3100.839 kb on - strand at 3101.009 kb on + strand, within DvMF_2458 at 3101.258 kb on - strand, within DvMF_2458 at 3101.493 kb on - strand, within DvMF_2458 at 3102.053 kb on + strand, within DvMF_2459 at 3102.061 kb on - strand, within DvMF_2459 at 3102.061 kb on - strand, within DvMF_2459 at 3102.063 kb on - strand, within DvMF_2459 at 3102.073 kb on - strand, within DvMF_2459 at 3102.276 kb on + strand, within DvMF_2459 at 3102.333 kb on - strand, within DvMF_2459 at 3102.500 kb on + strand, within DvMF_2459 at 3102.614 kb on - strand, within DvMF_2459 at 3102.745 kb on - strand, within DvMF_2459 at 3102.967 kb on + strand, within DvMF_2459 at 3102.975 kb on - strand, within DvMF_2459 at 3103.021 kb on - strand, within DvMF_2459 at 3103.045 kb on - strand, within DvMF_2459 at 3103.050 kb on - strand, within DvMF_2459 at 3103.070 kb on + strand, within DvMF_2459 at 3103.102 kb on - strand, within DvMF_2459 at 3103.110 kb on + strand, within DvMF_2459 at 3103.138 kb on + strand, within DvMF_2459 at 3103.224 kb on + strand at 3103.232 kb on - strand at 3103.340 kb on + strand at 3103.398 kb on + strand at 3103.679 kb on + strand, within frdB at 3103.819 kb on - strand, within frdB at 3104.071 kb on - strand, within frdB at 3104.241 kb on + strand at 3104.249 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Sisomicin 0.03125 mg/ml remove 3,100,839 - -0.6 3,101,009 + DvMF_2458 0.15 -1.3 3,101,258 - DvMF_2458 0.53 +1.5 3,101,493 - DvMF_2458 0.89 -1.0 3,102,053 + DvMF_2459 0.26 +0.3 3,102,061 - DvMF_2459 0.27 -0.9 3,102,061 - DvMF_2459 0.27 +1.4 3,102,063 - DvMF_2459 0.27 -0.4 3,102,073 - DvMF_2459 0.27 +0.3 3,102,276 + DvMF_2459 0.39 -0.8 3,102,333 - DvMF_2459 0.42 -0.2 3,102,500 + DvMF_2459 0.51 -0.5 3,102,614 - DvMF_2459 0.57 -1.3 3,102,745 - DvMF_2459 0.64 -0.3 3,102,967 + DvMF_2459 0.77 -0.9 3,102,975 - DvMF_2459 0.77 -0.1 3,103,021 - DvMF_2459 0.79 -0.3 3,103,045 - DvMF_2459 0.81 -0.5 3,103,050 - DvMF_2459 0.81 +0.6 3,103,070 + DvMF_2459 0.82 -0.3 3,103,102 - DvMF_2459 0.84 -0.6 3,103,110 + DvMF_2459 0.84 +0.1 3,103,138 + DvMF_2459 0.86 -0.4 3,103,224 + -0.6 3,103,232 - -0.7 3,103,340 + -0.3 3,103,398 + -0.7 3,103,679 + frdB DvMF_2460 0.18 -0.4 3,103,819 - frdB DvMF_2460 0.37 -0.4 3,104,071 - frdB DvMF_2460 0.71 +0.2 3,104,241 + -1.1 3,104,249 - -0.6
Or see this region's nucleotide sequence