Experiment: MoYLS4 with Sisomicin 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2222 and DvMF_2223 are separated by 263 nucleotides DvMF_2223 and DvMF_R0061 are separated by 112 nucleotides DvMF_R0061 and DvMF_2224 are separated by 97 nucleotides DvMF_2224 and DvMF_2225 are separated by 446 nucleotides
DvMF_2222: DvMF_2222 - protein of unknown function DUF214 (RefSeq), at 2,781,487 to 2,784,294
_2222
DvMF_2223: DvMF_2223 - diacylglycerol kinase (RefSeq), at 2,784,558 to 2,784,920
_2223
DvMF_R0061: DvMF_R0061 - tRNA-Lys (RefSeq), at 2,785,033 to 2,785,108
_R0061
DvMF_2224: DvMF_2224 - seryl-tRNA synthetase (RefSeq), at 2,785,206 to 2,786,483
_2224
DvMF_2225: DvMF_2225 - hypothetical protein (RefSeq), at 2,786,930 to 2,787,349
_2225
Position (kb)
2785
2786
2787 Strain fitness (log2 ratio)
-2
-1
0
1 at 2784.222 kb on - strand at 2784.369 kb on - strand at 2784.503 kb on + strand at 2784.555 kb on + strand at 2786.203 kb on + strand, within DvMF_2224 at 2786.918 kb on + strand at 2786.968 kb on + strand at 2786.976 kb on - strand, within DvMF_2225 at 2787.260 kb on - strand, within DvMF_2225
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Sisomicin 0.03125 mg/ml remove 2,784,222 - +0.2 2,784,369 - -0.9 2,784,503 + -0.8 2,784,555 + -1.9 2,786,203 + DvMF_2224 0.78 +0.6 2,786,918 + -0.4 2,786,968 + -1.2 2,786,976 - DvMF_2225 0.11 +0.2 2,787,260 - DvMF_2225 0.79 -0.1
Or see this region's nucleotide sequence