Experiment: MoYLS4 with Sisomicin 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1801 and DvMF_1802 are separated by 719 nucleotides DvMF_1802 and DvMF_1803 are separated by 164 nucleotides DvMF_1803 and DvMF_1804 are separated by 67 nucleotides
DvMF_1801: DvMF_1801 - phage shock protein C, PspC (RefSeq), at 2,201,607 to 2,202,188
_1801
DvMF_1802: DvMF_1802 - protein of unknown function DUF6 transmembrane (RefSeq), at 2,202,908 to 2,204,005
_1802
DvMF_1803: DvMF_1803 - alkyl hydroperoxide reductase/ Thiol specific antioxidant/ Mal allergen (RefSeq), at 2,204,170 to 2,204,874
_1803
DvMF_1804: DvMF_1804 - alkyl hydroperoxide reductase/ Thiol specific antioxidant/ Mal allergen (RefSeq), at 2,204,942 to 2,205,847
_1804
Position (kb)
2202
2203
2204
2205 Strain fitness (log2 ratio)
-2
-1
0
1 at 2202.284 kb on - strand at 2202.335 kb on - strand at 2202.370 kb on - strand at 2202.572 kb on - strand at 2202.679 kb on - strand at 2203.289 kb on - strand, within DvMF_1802 at 2203.448 kb on + strand, within DvMF_1802 at 2203.509 kb on + strand, within DvMF_1802 at 2203.746 kb on - strand, within DvMF_1802 at 2203.779 kb on + strand, within DvMF_1802 at 2203.881 kb on - strand, within DvMF_1802 at 2203.996 kb on - strand at 2204.074 kb on - strand at 2204.076 kb on + strand at 2204.103 kb on + strand at 2204.111 kb on - strand at 2204.173 kb on - strand at 2204.542 kb on - strand, within DvMF_1803 at 2204.599 kb on + strand, within DvMF_1803 at 2204.604 kb on - strand, within DvMF_1803 at 2204.782 kb on + strand, within DvMF_1803 at 2204.945 kb on + strand at 2204.976 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Sisomicin 0.03125 mg/ml remove 2,202,284 - -0.1 2,202,335 - -0.8 2,202,370 - -0.3 2,202,572 - -0.6 2,202,679 - -0.1 2,203,289 - DvMF_1802 0.35 -1.4 2,203,448 + DvMF_1802 0.49 -1.1 2,203,509 + DvMF_1802 0.55 -1.0 2,203,746 - DvMF_1802 0.76 -0.7 2,203,779 + DvMF_1802 0.79 -0.4 2,203,881 - DvMF_1802 0.89 -0.8 2,203,996 - -0.4 2,204,074 - -0.7 2,204,076 + -1.8 2,204,103 + +0.9 2,204,111 - -0.5 2,204,173 - +0.6 2,204,542 - DvMF_1803 0.53 -1.3 2,204,599 + DvMF_1803 0.61 -0.5 2,204,604 - DvMF_1803 0.62 -1.1 2,204,782 + DvMF_1803 0.87 +0.4 2,204,945 + -0.9 2,204,976 - +1.3
Or see this region's nucleotide sequence