Experiment: MoYLS4 with Sisomicin 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0751 and DvMF_0752 are separated by 8 nucleotides DvMF_0752 and DvMF_0753 are separated by 285 nucleotides DvMF_0753 and DvMF_0754 overlap by 35 nucleotides DvMF_0754 and DvMF_0755 are separated by 42 nucleotides
DvMF_0751: DvMF_0751 - hypothetical protein (RefSeq), at 897,835 to 899,127
_0751
DvMF_0752: DvMF_0752 - hypothetical protein (RefSeq), at 899,136 to 899,693
_0752
DvMF_0753: DvMF_0753 - hypothetical protein (RefSeq), at 899,979 to 900,185
_0753
DvMF_0754: DvMF_0754 - putative DNA modification protein (RefSeq), at 900,151 to 900,882
_0754
DvMF_0755: DvMF_0755 - hypothetical protein (RefSeq), at 900,925 to 901,131
_0755
Position (kb) 899
900
901 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 899.146 kb on - strand at 899.158 kb on - strand at 899.211 kb on - strand, within DvMF_0752 at 899.219 kb on - strand, within DvMF_0752 at 899.248 kb on - strand, within DvMF_0752 at 899.930 kb on - strand at 899.931 kb on - strand at 900.161 kb on - strand at 900.161 kb on - strand at 900.161 kb on - strand at 900.194 kb on - strand at 900.199 kb on + strand at 900.553 kb on + strand, within DvMF_0754 at 900.688 kb on + strand, within DvMF_0754 at 900.792 kb on + strand, within DvMF_0754 at 900.832 kb on + strand at 901.055 kb on - strand, within DvMF_0755 at 901.137 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Sisomicin 0.03125 mg/ml remove 899,146 - +0.3 899,158 - -3.0 899,211 - DvMF_0752 0.13 -0.4 899,219 - DvMF_0752 0.15 +0.1 899,248 - DvMF_0752 0.20 -0.2 899,930 - +0.6 899,931 - -0.7 900,161 - -0.9 900,161 - -0.8 900,161 - -0.9 900,194 - -0.5 900,199 + -0.2 900,553 + DvMF_0754 0.55 -0.2 900,688 + DvMF_0754 0.73 -1.4 900,792 + DvMF_0754 0.88 -0.6 900,832 + -1.5 901,055 - DvMF_0755 0.63 -2.8 901,137 + +0.5
Or see this region's nucleotide sequence