Experiment: MoYLS4 with Sisomicin 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0536 and DvMF_0537 are separated by 82 nucleotides DvMF_0537 and DvMF_0538 are separated by 10 nucleotides DvMF_0538 and DvMF_0539 are separated by 68 nucleotides DvMF_0539 and secF are separated by 256 nucleotides
DvMF_0536: DvMF_0536 - cytochrome c oxidase, subunit I (RefSeq), at 631,256 to 632,881
_0536
DvMF_0537: DvMF_0537 - Uncharacterized protein involved in biogenesis of respiratory and photosynthetic systems-like protein (RefSeq), at 632,964 to 634,211
_0537
DvMF_0538: DvMF_0538 - cytochrome c class I (RefSeq), at 634,222 to 634,530
_0538
DvMF_0539: DvMF_0539 - cytochrome c class I (RefSeq), at 634,599 to 634,925
_0539
DvMF_0540: secF - preprotein translocase subunit SecF (RefSeq), at 635,182 to 636,267
secF
Position (kb) 632
633
634
635 Strain fitness (log2 ratio) -2
-1
0
1 at 632.184 kb on - strand, within DvMF_0536 at 632.235 kb on - strand, within DvMF_0536 at 632.245 kb on + strand, within DvMF_0536 at 632.290 kb on + strand, within DvMF_0536 at 632.421 kb on + strand, within DvMF_0536 at 632.640 kb on - strand, within DvMF_0536 at 632.739 kb on + strand at 633.736 kb on - strand, within DvMF_0537 at 633.897 kb on + strand, within DvMF_0537 at 634.391 kb on + strand, within DvMF_0538 at 634.399 kb on - strand, within DvMF_0538 at 634.399 kb on - strand, within DvMF_0538 at 634.494 kb on - strand, within DvMF_0538 at 634.546 kb on - strand at 634.679 kb on + strand, within DvMF_0539 at 635.081 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Sisomicin 0.03125 mg/ml remove 632,184 - DvMF_0536 0.57 +0.3 632,235 - DvMF_0536 0.60 -1.8 632,245 + DvMF_0536 0.61 -1.0 632,290 + DvMF_0536 0.64 -1.1 632,421 + DvMF_0536 0.72 -0.9 632,640 - DvMF_0536 0.85 -0.5 632,739 + -0.3 633,736 - DvMF_0537 0.62 -0.3 633,897 + DvMF_0537 0.75 -0.5 634,391 + DvMF_0538 0.55 -2.4 634,399 - DvMF_0538 0.57 -0.6 634,399 - DvMF_0538 0.57 -1.1 634,494 - DvMF_0538 0.88 -1.3 634,546 - -1.0 634,679 + DvMF_0539 0.24 -1.0 635,081 + -0.9
Or see this region's nucleotide sequence