Experiment: MoYLS4 with Sisomicin 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0049 and DvMF_0050 are separated by 278 nucleotides DvMF_0050 and DvMF_0051 are separated by 226 nucleotides DvMF_0051 and DvMF_0052 are separated by 756 nucleotides
DvMF_0049: DvMF_0049 - hypothetical protein (RefSeq), at 65,645 to 66,274
_0049
DvMF_0050: DvMF_0050 - RNP-1 like RNA-binding protein (RefSeq), at 66,553 to 66,819
_0050
DvMF_0051: DvMF_0051 - extracellular solute-binding protein family 3 (RefSeq), at 67,046 to 67,840
_0051
DvMF_0052: DvMF_0052 - hypothetical protein (RefSeq), at 68,597 to 68,755
_0052
Position (kb) 67
68 Strain fitness (log2 ratio) -1
0
1 at 66.116 kb on + strand, within DvMF_0049 at 66.136 kb on + strand, within DvMF_0049 at 66.136 kb on + strand, within DvMF_0049 at 66.271 kb on - strand at 66.439 kb on + strand at 66.500 kb on - strand at 67.057 kb on + strand at 67.072 kb on + strand at 67.211 kb on - strand, within DvMF_0051 at 67.211 kb on - strand, within DvMF_0051 at 67.402 kb on + strand, within DvMF_0051 at 67.740 kb on - strand, within DvMF_0051 at 67.839 kb on - strand at 68.206 kb on - strand at 68.346 kb on - strand at 68.541 kb on + strand at 68.543 kb on + strand at 68.606 kb on + strand at 68.784 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Sisomicin 0.03125 mg/ml remove 66,116 + DvMF_0049 0.75 -0.9 66,136 + DvMF_0049 0.78 -0.2 66,136 + DvMF_0049 0.78 -1.1 66,271 - -0.0 66,439 + +0.3 66,500 - -0.7 67,057 + -1.2 67,072 + -0.4 67,211 - DvMF_0051 0.21 -0.7 67,211 - DvMF_0051 0.21 +0.2 67,402 + DvMF_0051 0.45 -0.3 67,740 - DvMF_0051 0.87 +0.1 67,839 - -1.0 68,206 - -1.2 68,346 - -0.1 68,541 + -0.3 68,543 + -0.3 68,606 + -1.0 68,784 + +0.6
Or see this region's nucleotide sequence