Experiment: MoYLS4 with Sisomicin 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0015 and DvMF_0016 overlap by 4 nucleotides DvMF_0016 and DvMF_0017 are separated by 71 nucleotides DvMF_0017 and DvMF_0018 are separated by 3 nucleotides DvMF_0018 and DvMF_0019 are separated by 37 nucleotides
DvMF_0015: DvMF_0015 - peptidase C26 (RefSeq), at 21,211 to 21,834
_0015
DvMF_0016: DvMF_0016 - transferase, putative (RefSeq), at 21,831 to 22,622
_0016
DvMF_0017: DvMF_0017 - Methyltransferase type 11 (RefSeq), at 22,694 to 23,458
_0017
DvMF_0018: DvMF_0018 - adenylylsulfate kinase (RefSeq), at 23,462 to 24,049
_0018
DvMF_0019: DvMF_0019 - hypothetical protein (RefSeq), at 24,087 to 25,421
_0019
Position (kb)
22
23
24 Strain fitness (log2 ratio)
-2
-1
0
1 at 21.836 kb on - strand at 22.011 kb on - strand, within DvMF_0016 at 22.263 kb on + strand, within DvMF_0016 at 22.379 kb on + strand, within DvMF_0016 at 22.399 kb on - strand, within DvMF_0016 at 22.399 kb on - strand, within DvMF_0016 at 22.399 kb on - strand, within DvMF_0016 at 23.086 kb on + strand, within DvMF_0017 at 23.304 kb on + strand, within DvMF_0017 at 23.334 kb on + strand, within DvMF_0017 at 23.460 kb on - strand at 23.723 kb on - strand, within DvMF_0018 at 24.058 kb on + strand at 24.058 kb on + strand at 24.066 kb on - strand at 24.066 kb on - strand at 24.109 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Sisomicin 0.03125 mg/ml remove 21,836 - -0.9 22,011 - DvMF_0016 0.23 -0.3 22,263 + DvMF_0016 0.55 -0.0 22,379 + DvMF_0016 0.69 -1.6 22,399 - DvMF_0016 0.72 -1.3 22,399 - DvMF_0016 0.72 -0.6 22,399 - DvMF_0016 0.72 -2.5 23,086 + DvMF_0017 0.51 +1.6 23,304 + DvMF_0017 0.80 -0.5 23,334 + DvMF_0017 0.84 -0.5 23,460 - -1.7 23,723 - DvMF_0018 0.44 -2.5 24,058 + -0.5 24,058 + -0.6 24,066 - -1.7 24,066 - -0.7 24,109 - -0.7
Or see this region's nucleotide sequence