Experiment: R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS23260 and CA265_RS23265 are separated by 172 nucleotides CA265_RS23265 and CA265_RS23270 are separated by 84 nucleotides CA265_RS23270 and CA265_RS23275 are separated by 78 nucleotides
CA265_RS23260: CA265_RS23260 - AraC family transcriptional regulator, at 5,685,794 to 5,686,597
_RS23260
CA265_RS23265: CA265_RS23265 - iron-binding protein, at 5,686,770 to 5,686,994
_RS23265
CA265_RS23270: CA265_RS23270 - hypothetical protein, at 5,687,079 to 5,687,762
_RS23270
CA265_RS23275: CA265_RS23275 - alpha/beta hydrolase, at 5,687,841 to 5,688,623
_RS23275
Position (kb) 5686
5687 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5685.837 kb on - strand at 5685.998 kb on + strand, within CA265_RS23260 at 5686.076 kb on + strand, within CA265_RS23260 at 5686.076 kb on + strand, within CA265_RS23260 at 5686.077 kb on - strand, within CA265_RS23260 at 5686.261 kb on - strand, within CA265_RS23260 at 5686.316 kb on + strand, within CA265_RS23260 at 5686.317 kb on - strand, within CA265_RS23260 at 5686.317 kb on - strand, within CA265_RS23260 at 5686.318 kb on + strand, within CA265_RS23260 at 5686.318 kb on + strand, within CA265_RS23260 at 5686.318 kb on + strand, within CA265_RS23260 at 5686.319 kb on - strand, within CA265_RS23260 at 5686.319 kb on - strand, within CA265_RS23260 at 5686.319 kb on - strand, within CA265_RS23260 at 5686.319 kb on - strand, within CA265_RS23260 at 5686.333 kb on + strand, within CA265_RS23260 at 5686.344 kb on + strand, within CA265_RS23260 at 5686.467 kb on - strand, within CA265_RS23260 at 5686.526 kb on - strand at 5686.596 kb on - strand at 5686.598 kb on + strand at 5686.633 kb on - strand at 5686.660 kb on - strand at 5686.683 kb on + strand at 5686.695 kb on + strand at 5686.696 kb on - strand at 5686.730 kb on - strand at 5686.732 kb on - strand at 5686.740 kb on + strand at 5686.740 kb on + strand at 5686.742 kb on + strand at 5686.801 kb on - strand at 5686.884 kb on + strand, within CA265_RS23265 at 5686.899 kb on + strand, within CA265_RS23265 at 5686.906 kb on + strand, within CA265_RS23265 at 5686.996 kb on + strand at 5686.996 kb on + strand at 5686.997 kb on - strand at 5687.019 kb on + strand at 5687.075 kb on + strand at 5687.080 kb on + strand at 5687.081 kb on - strand at 5687.081 kb on - strand at 5687.102 kb on - strand at 5687.139 kb on - strand at 5687.246 kb on - strand, within CA265_RS23270 at 5687.256 kb on + strand, within CA265_RS23270 at 5687.286 kb on + strand, within CA265_RS23270 at 5687.287 kb on - strand, within CA265_RS23270 at 5687.362 kb on + strand, within CA265_RS23270 at 5687.363 kb on - strand, within CA265_RS23270 at 5687.436 kb on - strand, within CA265_RS23270 at 5687.479 kb on - strand, within CA265_RS23270 at 5687.532 kb on + strand, within CA265_RS23270 at 5687.532 kb on + strand, within CA265_RS23270 at 5687.533 kb on - strand, within CA265_RS23270 at 5687.610 kb on + strand, within CA265_RS23270 at 5687.611 kb on - strand, within CA265_RS23270 at 5687.643 kb on + strand, within CA265_RS23270 at 5687.675 kb on - strand, within CA265_RS23270 at 5687.678 kb on - strand, within CA265_RS23270 at 5687.711 kb on + strand at 5687.712 kb on - strand at 5687.732 kb on - strand at 5687.774 kb on - strand at 5687.824 kb on + strand at 5687.898 kb on + strand at 5687.899 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A remove 5,685,837 - +1.8 5,685,998 + CA265_RS23260 0.25 -0.6 5,686,076 + CA265_RS23260 0.35 -0.8 5,686,076 + CA265_RS23260 0.35 -1.3 5,686,077 - CA265_RS23260 0.35 +0.0 5,686,261 - CA265_RS23260 0.58 +0.5 5,686,316 + CA265_RS23260 0.65 -0.9 5,686,317 - CA265_RS23260 0.65 -0.4 5,686,317 - CA265_RS23260 0.65 +0.1 5,686,318 + CA265_RS23260 0.65 -0.9 5,686,318 + CA265_RS23260 0.65 -0.1 5,686,318 + CA265_RS23260 0.65 -0.2 5,686,319 - CA265_RS23260 0.65 -1.0 5,686,319 - CA265_RS23260 0.65 +0.3 5,686,319 - CA265_RS23260 0.65 -0.3 5,686,319 - CA265_RS23260 0.65 +1.0 5,686,333 + CA265_RS23260 0.67 -0.9 5,686,344 + CA265_RS23260 0.68 -1.0 5,686,467 - CA265_RS23260 0.84 +0.0 5,686,526 - +0.4 5,686,596 - -0.6 5,686,598 + +1.6 5,686,633 - +0.3 5,686,660 - +0.5 5,686,683 + -0.1 5,686,695 + +0.7 5,686,696 - +0.2 5,686,730 - -0.5 5,686,732 - -2.1 5,686,740 + +0.9 5,686,740 + +0.0 5,686,742 + +0.7 5,686,801 - +0.4 5,686,884 + CA265_RS23265 0.51 +0.6 5,686,899 + CA265_RS23265 0.57 +0.6 5,686,906 + CA265_RS23265 0.60 +0.4 5,686,996 + -0.1 5,686,996 + -0.5 5,686,997 - +1.0 5,687,019 + +1.2 5,687,075 + +0.2 5,687,080 + -0.8 5,687,081 - -1.2 5,687,081 - +1.3 5,687,102 - -0.1 5,687,139 - -0.8 5,687,246 - CA265_RS23270 0.24 +0.7 5,687,256 + CA265_RS23270 0.26 -0.1 5,687,286 + CA265_RS23270 0.30 -0.7 5,687,287 - CA265_RS23270 0.30 -0.0 5,687,362 + CA265_RS23270 0.41 +1.5 5,687,363 - CA265_RS23270 0.42 -1.9 5,687,436 - CA265_RS23270 0.52 +0.6 5,687,479 - CA265_RS23270 0.58 -0.5 5,687,532 + CA265_RS23270 0.66 +0.4 5,687,532 + CA265_RS23270 0.66 -0.6 5,687,533 - CA265_RS23270 0.66 +0.3 5,687,610 + CA265_RS23270 0.78 +0.4 5,687,611 - CA265_RS23270 0.78 -0.5 5,687,643 + CA265_RS23270 0.82 +1.0 5,687,675 - CA265_RS23270 0.87 +0.3 5,687,678 - CA265_RS23270 0.88 +0.8 5,687,711 + +2.0 5,687,712 - -0.3 5,687,732 - -2.3 5,687,774 - +0.3 5,687,824 + -0.1 5,687,898 + +1.6 5,687,899 - +0.3
Or see this region's nucleotide sequence