Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1338 and Pf6N2E2_1339 are separated by 181 nucleotides Pf6N2E2_1339 and Pf6N2E2_1340 overlap by 23 nucleotides Pf6N2E2_1340 and Pf6N2E2_1341 are separated by 10 nucleotides
Pf6N2E2_1338 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 1,573,976 to 1,575,016
_1338
Pf6N2E2_1339 - Acetylornithine deacetylase (EC 3.5.1.16), at 1,575,198 to 1,576,382
_1339
Pf6N2E2_1340 - major pilin protein fimA, at 1,576,360 to 1,577,034
_1340
Pf6N2E2_1341 - Bona fide RidA/YjgF/TdcF/RutC subgroup, at 1,577,045 to 1,577,431
_1341
Position (kb)
1575
1576
1577 Strain fitness (log2 ratio)
-1
0
1
2 at 1574.469 kb on + strand, within Pf6N2E2_1338 at 1574.469 kb on + strand, within Pf6N2E2_1338 at 1574.470 kb on - strand, within Pf6N2E2_1338 at 1574.470 kb on - strand, within Pf6N2E2_1338 at 1574.470 kb on - strand, within Pf6N2E2_1338 at 1574.470 kb on - strand, within Pf6N2E2_1338 at 1574.520 kb on + strand, within Pf6N2E2_1338 at 1574.521 kb on - strand, within Pf6N2E2_1338 at 1574.521 kb on - strand, within Pf6N2E2_1338 at 1574.619 kb on + strand, within Pf6N2E2_1338 at 1574.620 kb on - strand, within Pf6N2E2_1338 at 1574.643 kb on + strand, within Pf6N2E2_1338 at 1574.643 kb on + strand, within Pf6N2E2_1338 at 1574.643 kb on + strand, within Pf6N2E2_1338 at 1574.643 kb on + strand, within Pf6N2E2_1338 at 1574.644 kb on - strand, within Pf6N2E2_1338 at 1574.644 kb on - strand, within Pf6N2E2_1338 at 1574.682 kb on + strand, within Pf6N2E2_1338 at 1574.682 kb on + strand, within Pf6N2E2_1338 at 1574.683 kb on - strand, within Pf6N2E2_1338 at 1574.683 kb on - strand, within Pf6N2E2_1338 at 1574.683 kb on - strand, within Pf6N2E2_1338 at 1574.683 kb on - strand, within Pf6N2E2_1338 at 1574.683 kb on - strand, within Pf6N2E2_1338 at 1574.835 kb on + strand, within Pf6N2E2_1338 at 1574.835 kb on + strand, within Pf6N2E2_1338 at 1574.836 kb on - strand, within Pf6N2E2_1338 at 1574.887 kb on - strand, within Pf6N2E2_1338 at 1574.993 kb on - strand at 1575.082 kb on - strand at 1575.082 kb on - strand at 1575.085 kb on + strand at 1575.102 kb on - strand at 1575.223 kb on + strand at 1575.223 kb on + strand at 1575.224 kb on - strand at 1575.224 kb on - strand at 1575.224 kb on - strand at 1575.280 kb on + strand at 1575.280 kb on + strand at 1575.281 kb on - strand at 1575.539 kb on - strand, within Pf6N2E2_1339 at 1576.231 kb on + strand, within Pf6N2E2_1339 at 1576.231 kb on + strand, within Pf6N2E2_1339 at 1576.267 kb on + strand at 1576.268 kb on - strand at 1576.268 kb on - strand at 1576.899 kb on + strand, within Pf6N2E2_1340 at 1576.900 kb on - strand, within Pf6N2E2_1340 at 1576.990 kb on + strand at 1577.199 kb on + strand, within Pf6N2E2_1341 at 1577.199 kb on + strand, within Pf6N2E2_1341 at 1577.199 kb on + strand, within Pf6N2E2_1341 at 1577.199 kb on + strand, within Pf6N2E2_1341 at 1577.200 kb on - strand, within Pf6N2E2_1341 at 1577.200 kb on - strand, within Pf6N2E2_1341 at 1577.200 kb on - strand, within Pf6N2E2_1341 at 1577.349 kb on + strand at 1577.349 kb on + strand, within Pf6N2E2_1341 at 1577.350 kb on - strand, within Pf6N2E2_1341 at 1577.350 kb on - strand, within Pf6N2E2_1341
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 1,574,469 + Pf6N2E2_1338 0.47 +1.6 1,574,469 + Pf6N2E2_1338 0.47 +0.6 1,574,470 - Pf6N2E2_1338 0.47 -0.2 1,574,470 - Pf6N2E2_1338 0.47 -0.4 1,574,470 - Pf6N2E2_1338 0.47 -0.3 1,574,470 - Pf6N2E2_1338 0.47 -0.5 1,574,520 + Pf6N2E2_1338 0.52 +0.1 1,574,521 - Pf6N2E2_1338 0.52 +1.3 1,574,521 - Pf6N2E2_1338 0.52 +0.2 1,574,619 + Pf6N2E2_1338 0.62 +0.4 1,574,620 - Pf6N2E2_1338 0.62 -0.3 1,574,643 + Pf6N2E2_1338 0.64 -0.5 1,574,643 + Pf6N2E2_1338 0.64 +0.3 1,574,643 + Pf6N2E2_1338 0.64 +1.5 1,574,643 + Pf6N2E2_1338 0.64 -0.0 1,574,644 - Pf6N2E2_1338 0.64 +0.3 1,574,644 - Pf6N2E2_1338 0.64 +1.0 1,574,682 + Pf6N2E2_1338 0.68 +2.7 1,574,682 + Pf6N2E2_1338 0.68 +1.1 1,574,683 - Pf6N2E2_1338 0.68 -1.0 1,574,683 - Pf6N2E2_1338 0.68 -0.2 1,574,683 - Pf6N2E2_1338 0.68 +0.3 1,574,683 - Pf6N2E2_1338 0.68 -0.3 1,574,683 - Pf6N2E2_1338 0.68 +0.1 1,574,835 + Pf6N2E2_1338 0.83 -0.2 1,574,835 + Pf6N2E2_1338 0.83 -0.4 1,574,836 - Pf6N2E2_1338 0.83 -1.5 1,574,887 - Pf6N2E2_1338 0.88 +0.1 1,574,993 - +2.0 1,575,082 - +0.9 1,575,082 - +0.2 1,575,085 + +0.2 1,575,102 - +0.2 1,575,223 + +1.5 1,575,223 + +0.3 1,575,224 - -0.2 1,575,224 - -0.1 1,575,224 - -0.5 1,575,280 + +0.5 1,575,280 + -0.2 1,575,281 - +0.1 1,575,539 - Pf6N2E2_1339 0.29 -0.2 1,576,231 + Pf6N2E2_1339 0.87 -1.0 1,576,231 + Pf6N2E2_1339 0.87 -1.1 1,576,267 + +0.4 1,576,268 - +0.1 1,576,268 - +1.0 1,576,899 + Pf6N2E2_1340 0.80 +0.1 1,576,900 - Pf6N2E2_1340 0.80 +0.6 1,576,990 + +0.1 1,577,199 + Pf6N2E2_1341 0.40 +0.4 1,577,199 + Pf6N2E2_1341 0.40 +0.6 1,577,199 + Pf6N2E2_1341 0.40 +0.0 1,577,199 + Pf6N2E2_1341 0.40 -1.4 1,577,200 - Pf6N2E2_1341 0.40 -0.2 1,577,200 - Pf6N2E2_1341 0.40 -0.2 1,577,200 - Pf6N2E2_1341 0.40 -0.1 1,577,349 + +0.4 1,577,349 + Pf6N2E2_1341 0.79 -1.4 1,577,350 - Pf6N2E2_1341 0.79 +0.7 1,577,350 - Pf6N2E2_1341 0.79 +0.4
Or see this region's nucleotide sequence