Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1139 and Pf6N2E2_1140 are separated by 33 nucleotides Pf6N2E2_1140 and Pf6N2E2_1141 are separated by 1 nucleotides Pf6N2E2_1141 and Pf6N2E2_1142 are separated by 49 nucleotides
Pf6N2E2_1139 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 1,335,759 to 1,336,730
_1139
Pf6N2E2_1140 - Polymyxin resistance protein ArnC, glycosyl transferase (EC 2.4.-.-), at 1,336,764 to 1,337,507
_1140
Pf6N2E2_1141 - Lipid-A-disaccharide synthase (EC 2.4.1.182), at 1,337,509 to 1,337,802
_1141
Pf6N2E2_1142 - Polymyxin resistance protein ArnT, undecaprenyl phosphate-alpha-L-Ara4N transferase; Melittin resistance protein PqaB, at 1,337,852 to 1,339,327
_1142
Position (kb)
1336
1337
1338 Strain fitness (log2 ratio)
-2
-1
0
1 at 1335.865 kb on - strand, within Pf6N2E2_1139 at 1335.867 kb on + strand, within Pf6N2E2_1139 at 1335.867 kb on + strand, within Pf6N2E2_1139 at 1335.868 kb on - strand, within Pf6N2E2_1139 at 1335.868 kb on - strand, within Pf6N2E2_1139 at 1336.053 kb on + strand, within Pf6N2E2_1139 at 1336.054 kb on - strand, within Pf6N2E2_1139 at 1336.072 kb on + strand, within Pf6N2E2_1139 at 1336.072 kb on + strand, within Pf6N2E2_1139 at 1336.073 kb on - strand, within Pf6N2E2_1139 at 1336.174 kb on - strand, within Pf6N2E2_1139 at 1336.266 kb on + strand, within Pf6N2E2_1139 at 1336.305 kb on + strand, within Pf6N2E2_1139 at 1336.305 kb on + strand, within Pf6N2E2_1139 at 1336.306 kb on - strand, within Pf6N2E2_1139 at 1336.306 kb on - strand, within Pf6N2E2_1139 at 1336.416 kb on + strand, within Pf6N2E2_1139 at 1336.417 kb on - strand, within Pf6N2E2_1139 at 1336.417 kb on - strand, within Pf6N2E2_1139 at 1336.417 kb on - strand, within Pf6N2E2_1139 at 1336.417 kb on - strand, within Pf6N2E2_1139 at 1336.417 kb on - strand, within Pf6N2E2_1139 at 1336.418 kb on + strand, within Pf6N2E2_1139 at 1336.497 kb on + strand, within Pf6N2E2_1139 at 1336.498 kb on - strand, within Pf6N2E2_1139 at 1336.498 kb on - strand, within Pf6N2E2_1139 at 1336.498 kb on - strand, within Pf6N2E2_1139 at 1336.498 kb on - strand, within Pf6N2E2_1139 at 1336.707 kb on + strand at 1336.716 kb on + strand at 1336.719 kb on + strand at 1336.720 kb on - strand at 1336.779 kb on + strand at 1336.779 kb on + strand at 1336.780 kb on - strand at 1336.780 kb on - strand at 1336.780 kb on - strand at 1336.783 kb on + strand at 1336.783 kb on + strand at 1336.784 kb on - strand at 1336.817 kb on + strand at 1336.817 kb on + strand at 1336.921 kb on + strand, within Pf6N2E2_1140 at 1336.922 kb on - strand, within Pf6N2E2_1140 at 1336.922 kb on - strand, within Pf6N2E2_1140 at 1337.001 kb on + strand, within Pf6N2E2_1140 at 1337.590 kb on + strand, within Pf6N2E2_1141 at 1337.600 kb on - strand, within Pf6N2E2_1141 at 1337.600 kb on - strand, within Pf6N2E2_1141 at 1337.671 kb on + strand, within Pf6N2E2_1141 at 1337.671 kb on + strand, within Pf6N2E2_1141 at 1337.672 kb on - strand, within Pf6N2E2_1141 at 1337.856 kb on + strand at 1337.856 kb on + strand at 1337.856 kb on + strand at 1337.993 kb on + strand at 1338.071 kb on + strand, within Pf6N2E2_1142 at 1338.072 kb on - strand, within Pf6N2E2_1142 at 1338.072 kb on - strand, within Pf6N2E2_1142 at 1338.116 kb on + strand, within Pf6N2E2_1142 at 1338.116 kb on + strand, within Pf6N2E2_1142 at 1338.194 kb on + strand, within Pf6N2E2_1142 at 1338.194 kb on + strand, within Pf6N2E2_1142 at 1338.194 kb on + strand, within Pf6N2E2_1142 at 1338.257 kb on + strand, within Pf6N2E2_1142 at 1338.257 kb on + strand, within Pf6N2E2_1142 at 1338.257 kb on + strand, within Pf6N2E2_1142 at 1338.258 kb on - strand, within Pf6N2E2_1142 at 1338.258 kb on - strand, within Pf6N2E2_1142 at 1338.258 kb on - strand, within Pf6N2E2_1142 at 1338.302 kb on + strand, within Pf6N2E2_1142 at 1338.303 kb on - strand, within Pf6N2E2_1142 at 1338.305 kb on + strand, within Pf6N2E2_1142 at 1338.305 kb on + strand, within Pf6N2E2_1142 at 1338.306 kb on - strand, within Pf6N2E2_1142 at 1338.306 kb on - strand, within Pf6N2E2_1142 at 1338.369 kb on + strand, within Pf6N2E2_1142 at 1338.389 kb on + strand, within Pf6N2E2_1142 at 1338.389 kb on + strand, within Pf6N2E2_1142
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 1,335,865 - Pf6N2E2_1139 0.11 +0.8 1,335,867 + Pf6N2E2_1139 0.11 +1.0 1,335,867 + Pf6N2E2_1139 0.11 -0.8 1,335,868 - Pf6N2E2_1139 0.11 +0.7 1,335,868 - Pf6N2E2_1139 0.11 -0.4 1,336,053 + Pf6N2E2_1139 0.30 +0.7 1,336,054 - Pf6N2E2_1139 0.30 +0.1 1,336,072 + Pf6N2E2_1139 0.32 +0.9 1,336,072 + Pf6N2E2_1139 0.32 -2.1 1,336,073 - Pf6N2E2_1139 0.32 -0.8 1,336,174 - Pf6N2E2_1139 0.43 -0.8 1,336,266 + Pf6N2E2_1139 0.52 +0.0 1,336,305 + Pf6N2E2_1139 0.56 +0.3 1,336,305 + Pf6N2E2_1139 0.56 +0.8 1,336,306 - Pf6N2E2_1139 0.56 +0.3 1,336,306 - Pf6N2E2_1139 0.56 +0.1 1,336,416 + Pf6N2E2_1139 0.68 -0.1 1,336,417 - Pf6N2E2_1139 0.68 +0.4 1,336,417 - Pf6N2E2_1139 0.68 +0.6 1,336,417 - Pf6N2E2_1139 0.68 +0.2 1,336,417 - Pf6N2E2_1139 0.68 +0.3 1,336,417 - Pf6N2E2_1139 0.68 -0.2 1,336,418 + Pf6N2E2_1139 0.68 +0.8 1,336,497 + Pf6N2E2_1139 0.76 +0.6 1,336,498 - Pf6N2E2_1139 0.76 -0.2 1,336,498 - Pf6N2E2_1139 0.76 +1.3 1,336,498 - Pf6N2E2_1139 0.76 +0.1 1,336,498 - Pf6N2E2_1139 0.76 -0.0 1,336,707 + -0.0 1,336,716 + +0.3 1,336,719 + +1.3 1,336,720 - -0.8 1,336,779 + +0.0 1,336,779 + +0.3 1,336,780 - +0.0 1,336,780 - -0.1 1,336,780 - -1.9 1,336,783 + -0.1 1,336,783 + -0.2 1,336,784 - -1.0 1,336,817 + -0.1 1,336,817 + +0.3 1,336,921 + Pf6N2E2_1140 0.21 -0.5 1,336,922 - Pf6N2E2_1140 0.21 -0.5 1,336,922 - Pf6N2E2_1140 0.21 +0.4 1,337,001 + Pf6N2E2_1140 0.32 -1.0 1,337,590 + Pf6N2E2_1141 0.28 -0.2 1,337,600 - Pf6N2E2_1141 0.31 -0.9 1,337,600 - Pf6N2E2_1141 0.31 -1.6 1,337,671 + Pf6N2E2_1141 0.55 +0.9 1,337,671 + Pf6N2E2_1141 0.55 -0.1 1,337,672 - Pf6N2E2_1141 0.55 -0.4 1,337,856 + -0.1 1,337,856 + -0.6 1,337,856 + +0.9 1,337,993 + +1.0 1,338,071 + Pf6N2E2_1142 0.15 +0.0 1,338,072 - Pf6N2E2_1142 0.15 -0.8 1,338,072 - Pf6N2E2_1142 0.15 -0.1 1,338,116 + Pf6N2E2_1142 0.18 -0.1 1,338,116 + Pf6N2E2_1142 0.18 +0.5 1,338,194 + Pf6N2E2_1142 0.23 +0.7 1,338,194 + Pf6N2E2_1142 0.23 +0.9 1,338,194 + Pf6N2E2_1142 0.23 -0.3 1,338,257 + Pf6N2E2_1142 0.27 -1.9 1,338,257 + Pf6N2E2_1142 0.27 +0.2 1,338,257 + Pf6N2E2_1142 0.27 -1.4 1,338,258 - Pf6N2E2_1142 0.28 +1.2 1,338,258 - Pf6N2E2_1142 0.28 +1.0 1,338,258 - Pf6N2E2_1142 0.28 -0.1 1,338,302 + Pf6N2E2_1142 0.30 +0.6 1,338,303 - Pf6N2E2_1142 0.31 +0.4 1,338,305 + Pf6N2E2_1142 0.31 +1.6 1,338,305 + Pf6N2E2_1142 0.31 +0.1 1,338,306 - Pf6N2E2_1142 0.31 -1.4 1,338,306 - Pf6N2E2_1142 0.31 +0.6 1,338,369 + Pf6N2E2_1142 0.35 +0.3 1,338,389 + Pf6N2E2_1142 0.36 +0.3 1,338,389 + Pf6N2E2_1142 0.36 +1.4
Or see this region's nucleotide sequence