Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS20845

Experiment: CDM1 without Alanine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBBR_RS14690 and BBR_RS20845 are separated by 93 nucleotidesBBR_RS20845 and BBR_RS14700 are separated by 216 nucleotidesBBR_RS14700 and BBR_RS14705 overlap by 14 nucleotidesBBR_RS14705 and BBR_RS14710 are separated by 171 nucleotides BBR_RS14690: BBR_RS14690 - class I SAM-dependent methyltransferase, at 1,071,644 to 1,072,513 _RS14690 BBR_RS20845: BBR_RS20845 - cupin, at 1,072,607 to 1,073,221 _RS20845 BBR_RS14700: BBR_RS14700 - type II toxin-antitoxin system PemK/MazF family toxin, at 1,073,438 to 1,073,761 _RS14700 BBR_RS14705: BBR_RS14705 - translation repressor RelB, at 1,073,748 to 1,073,993 _RS14705 BBR_RS14710: BBR_RS14710 - hypothetical protein, at 1,074,165 to 1,075,421 _RS14710 Position (kb) 1072 1073 1074Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1071.648 kb on + strandat 1071.655 kb on + strandat 1071.656 kb on - strandat 1071.656 kb on - strandat 1071.656 kb on - strandat 1071.682 kb on - strandat 1071.682 kb on - strandat 1071.733 kb on + strand, within BBR_RS14690at 1071.741 kb on + strand, within BBR_RS14690at 1071.748 kb on + strand, within BBR_RS14690at 1071.748 kb on + strand, within BBR_RS14690at 1071.756 kb on - strand, within BBR_RS14690at 1071.756 kb on - strand, within BBR_RS14690at 1071.881 kb on + strand, within BBR_RS14690at 1071.881 kb on + strand, within BBR_RS14690at 1071.919 kb on + strand, within BBR_RS14690at 1071.972 kb on - strand, within BBR_RS14690at 1071.976 kb on - strand, within BBR_RS14690at 1071.993 kb on - strand, within BBR_RS14690at 1072.010 kb on - strand, within BBR_RS14690at 1072.030 kb on - strand, within BBR_RS14690at 1072.070 kb on + strand, within BBR_RS14690at 1072.131 kb on - strand, within BBR_RS14690at 1072.131 kb on - strand, within BBR_RS14690at 1072.164 kb on + strand, within BBR_RS14690at 1072.174 kb on + strand, within BBR_RS14690at 1072.185 kb on + strand, within BBR_RS14690at 1072.211 kb on - strand, within BBR_RS14690at 1072.213 kb on + strand, within BBR_RS14690at 1072.221 kb on - strand, within BBR_RS14690at 1072.290 kb on - strand, within BBR_RS14690at 1072.304 kb on - strand, within BBR_RS14690at 1072.306 kb on + strand, within BBR_RS14690at 1072.367 kb on + strand, within BBR_RS14690at 1072.367 kb on + strand, within BBR_RS14690at 1072.374 kb on + strand, within BBR_RS14690at 1072.400 kb on + strand, within BBR_RS14690at 1072.417 kb on + strand, within BBR_RS14690at 1072.442 kb on + strandat 1072.445 kb on - strandat 1072.445 kb on - strandat 1072.445 kb on - strandat 1072.445 kb on - strandat 1072.449 kb on + strandat 1072.452 kb on - strandat 1072.485 kb on + strandat 1072.611 kb on - strandat 1072.611 kb on - strandat 1072.632 kb on - strandat 1072.686 kb on - strand, within BBR_RS20845at 1072.730 kb on - strand, within BBR_RS20845at 1072.810 kb on - strand, within BBR_RS20845at 1072.846 kb on - strand, within BBR_RS20845at 1072.874 kb on + strand, within BBR_RS20845at 1072.874 kb on + strand, within BBR_RS20845at 1072.882 kb on - strand, within BBR_RS20845at 1072.921 kb on + strand, within BBR_RS20845at 1072.966 kb on - strand, within BBR_RS20845at 1072.968 kb on + strand, within BBR_RS20845at 1072.969 kb on - strand, within BBR_RS20845at 1072.971 kb on - strand, within BBR_RS20845at 1072.981 kb on - strand, within BBR_RS20845at 1072.998 kb on - strand, within BBR_RS20845at 1073.000 kb on + strand, within BBR_RS20845at 1073.003 kb on - strand, within BBR_RS20845at 1073.020 kb on + strand, within BBR_RS20845at 1073.035 kb on - strand, within BBR_RS20845at 1073.079 kb on - strand, within BBR_RS20845at 1073.135 kb on + strand, within BBR_RS20845at 1073.135 kb on + strand, within BBR_RS20845at 1073.141 kb on - strand, within BBR_RS20845at 1073.172 kb on - strandat 1073.172 kb on - strandat 1073.182 kb on + strandat 1073.243 kb on + strandat 1073.252 kb on - strandat 1073.270 kb on - strandat 1073.280 kb on - strandat 1073.306 kb on - strandat 1073.347 kb on - strandat 1073.363 kb on - strandat 1073.379 kb on + strandat 1073.379 kb on + strandat 1073.386 kb on - strandat 1073.389 kb on + strandat 1073.408 kb on - strandat 1073.436 kb on + strandat 1073.441 kb on + strandat 1073.544 kb on + strand, within BBR_RS14700at 1073.669 kb on - strand, within BBR_RS14700at 1073.679 kb on + strand, within BBR_RS14700at 1073.687 kb on - strand, within BBR_RS14700at 1073.693 kb on + strand, within BBR_RS14700at 1073.747 kb on - strandat 1073.768 kb on + strandat 1073.769 kb on + strandat 1074.044 kb on + strandat 1074.084 kb on + strandat 1074.084 kb on + strandat 1074.084 kb on + strandat 1074.084 kb on + strandat 1074.084 kb on + strandat 1074.089 kb on + strandat 1074.091 kb on + strandat 1074.091 kb on + strandat 1074.092 kb on - strandat 1074.097 kb on - strandat 1074.165 kb on - strandat 1074.180 kb on - strandat 1074.190 kb on + strandat 1074.216 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction CDM1 without Alanine
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1,071,648 + +0.3
1,071,655 + +0.2
1,071,656 - +0.8
1,071,656 - -0.2
1,071,656 - +0.6
1,071,682 - +0.8
1,071,682 - -1.2
1,071,733 + BBR_RS14690 0.10 -2.8
1,071,741 + BBR_RS14690 0.11 +0.0
1,071,748 + BBR_RS14690 0.12 +0.7
1,071,748 + BBR_RS14690 0.12 +0.3
1,071,756 - BBR_RS14690 0.13 -0.3
1,071,756 - BBR_RS14690 0.13 +1.0
1,071,881 + BBR_RS14690 0.27 +0.1
1,071,881 + BBR_RS14690 0.27 -1.3
1,071,919 + BBR_RS14690 0.32 +0.7
1,071,972 - BBR_RS14690 0.38 +0.6
1,071,976 - BBR_RS14690 0.38 -0.5
1,071,993 - BBR_RS14690 0.40 +0.1
1,072,010 - BBR_RS14690 0.42 -0.2
1,072,030 - BBR_RS14690 0.44 -0.2
1,072,070 + BBR_RS14690 0.49 -1.2
1,072,131 - BBR_RS14690 0.56 -0.7
1,072,131 - BBR_RS14690 0.56 -1.4
1,072,164 + BBR_RS14690 0.60 -1.2
1,072,174 + BBR_RS14690 0.61 -0.5
1,072,185 + BBR_RS14690 0.62 -0.7
1,072,211 - BBR_RS14690 0.65 -0.1
1,072,213 + BBR_RS14690 0.65 -1.3
1,072,221 - BBR_RS14690 0.66 -0.2
1,072,290 - BBR_RS14690 0.74 +0.3
1,072,304 - BBR_RS14690 0.76 +0.9
1,072,306 + BBR_RS14690 0.76 -1.0
1,072,367 + BBR_RS14690 0.83 -0.8
1,072,367 + BBR_RS14690 0.83 +0.2
1,072,374 + BBR_RS14690 0.84 -1.6
1,072,400 + BBR_RS14690 0.87 +0.9
1,072,417 + BBR_RS14690 0.89 -0.8
1,072,442 + +0.6
1,072,445 - +0.8
1,072,445 - +0.7
1,072,445 - +0.2
1,072,445 - -0.3
1,072,449 + +2.1
1,072,452 - -0.3
1,072,485 + +0.1
1,072,611 - +0.4
1,072,611 - -1.8
1,072,632 - +0.6
1,072,686 - BBR_RS20845 0.13 -1.6
1,072,730 - BBR_RS20845 0.20 -0.4
1,072,810 - BBR_RS20845 0.33 +1.3
1,072,846 - BBR_RS20845 0.39 +0.2
1,072,874 + BBR_RS20845 0.43 +0.3
1,072,874 + BBR_RS20845 0.43 -1.9
1,072,882 - BBR_RS20845 0.45 +0.2
1,072,921 + BBR_RS20845 0.51 +1.7
1,072,966 - BBR_RS20845 0.58 -1.1
1,072,968 + BBR_RS20845 0.59 +0.7
1,072,969 - BBR_RS20845 0.59 +2.1
1,072,971 - BBR_RS20845 0.59 -0.7
1,072,981 - BBR_RS20845 0.61 +1.1
1,072,998 - BBR_RS20845 0.64 +1.8
1,073,000 + BBR_RS20845 0.64 -0.2
1,073,003 - BBR_RS20845 0.64 +0.8
1,073,020 + BBR_RS20845 0.67 +0.7
1,073,035 - BBR_RS20845 0.70 +0.7
1,073,079 - BBR_RS20845 0.77 +0.9
1,073,135 + BBR_RS20845 0.86 +0.5
1,073,135 + BBR_RS20845 0.86 +0.6
1,073,141 - BBR_RS20845 0.87 -0.1
1,073,172 - +0.8
1,073,172 - +0.6
1,073,182 + -1.2
1,073,243 + +1.2
1,073,252 - +0.6
1,073,270 - +0.2
1,073,280 - +0.4
1,073,306 - +1.8
1,073,347 - +0.3
1,073,363 - -0.8
1,073,379 + -0.8
1,073,379 + -1.5
1,073,386 - -0.6
1,073,389 + -1.8
1,073,408 - +3.3
1,073,436 + -2.2
1,073,441 + +1.8
1,073,544 + BBR_RS14700 0.33 +1.4
1,073,669 - BBR_RS14700 0.71 -1.3
1,073,679 + BBR_RS14700 0.74 -0.2
1,073,687 - BBR_RS14700 0.77 -1.1
1,073,693 + BBR_RS14700 0.79 -1.5
1,073,747 - -2.5
1,073,768 + -0.3
1,073,769 + -0.2
1,074,044 + -1.2
1,074,084 + -1.6
1,074,084 + -3.2
1,074,084 + +0.5
1,074,084 + +0.9
1,074,084 + -0.5
1,074,089 + -2.0
1,074,091 + -0.9
1,074,091 + -0.6
1,074,092 - +0.4
1,074,097 - +1.6
1,074,165 - -0.5
1,074,180 - -0.1
1,074,190 + +0.9
1,074,216 + -1.7

Or see this region's nucleotide sequence