Experiment: MoYLS4 with nitrite 1.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1871 and DvMF_R0050 are separated by 249 nucleotides DvMF_R0050 and DvMF_1872 are separated by 75 nucleotides DvMF_1872 and DvMF_1873 are separated by 410 nucleotides
DvMF_1871: DvMF_1871 - Rhodanese domain protein (RefSeq), at 2,297,570 to 2,298,598
_1871
DvMF_R0050: DvMF_R0050 - tRNA-Pro (RefSeq), at 2,298,848 to 2,298,924
_R0050
DvMF_1872: DvMF_1872 - Acetamidase/Formamidase (RefSeq), at 2,299,000 to 2,300,289
_1872
DvMF_1873: DvMF_1873 - Radical SAM domain protein (RefSeq), at 2,300,700 to 2,302,052
_1873
Position (kb)
2298
2299
2300
2301 Strain fitness (log2 ratio)
-2
-1
0
1 at 2298.040 kb on - strand, within DvMF_1871 at 2298.612 kb on - strand at 2298.716 kb on - strand at 2298.743 kb on + strand at 2299.016 kb on + strand at 2299.103 kb on + strand at 2299.211 kb on + strand, within DvMF_1872 at 2299.234 kb on - strand, within DvMF_1872 at 2299.484 kb on - strand, within DvMF_1872 at 2299.664 kb on + strand, within DvMF_1872 at 2299.816 kb on - strand, within DvMF_1872 at 2299.820 kb on - strand, within DvMF_1872 at 2299.983 kb on + strand, within DvMF_1872 at 2300.102 kb on - strand, within DvMF_1872 at 2300.159 kb on - strand, within DvMF_1872 at 2300.274 kb on - strand at 2300.451 kb on + strand at 2300.722 kb on + strand at 2300.792 kb on + strand at 2300.921 kb on - strand, within DvMF_1873 at 2301.076 kb on + strand, within DvMF_1873 at 2301.076 kb on + strand, within DvMF_1873 at 2301.076 kb on + strand, within DvMF_1873 at 2301.076 kb on + strand, within DvMF_1873 at 2301.084 kb on - strand, within DvMF_1873 at 2301.113 kb on + strand, within DvMF_1873 at 2301.197 kb on - strand, within DvMF_1873 at 2301.264 kb on + strand, within DvMF_1873
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with nitrite 1.25 mg/ml remove 2,298,040 - DvMF_1871 0.46 +0.0 2,298,612 - -0.3 2,298,716 - +0.8 2,298,743 + -2.2 2,299,016 + +0.1 2,299,103 + +0.5 2,299,211 + DvMF_1872 0.16 +0.4 2,299,234 - DvMF_1872 0.18 -0.4 2,299,484 - DvMF_1872 0.38 -0.2 2,299,664 + DvMF_1872 0.51 -0.1 2,299,816 - DvMF_1872 0.63 +0.2 2,299,820 - DvMF_1872 0.64 +0.6 2,299,983 + DvMF_1872 0.76 +0.4 2,300,102 - DvMF_1872 0.85 -0.4 2,300,159 - DvMF_1872 0.90 -0.1 2,300,274 - -0.5 2,300,451 + -0.3 2,300,722 + +0.5 2,300,792 + -0.4 2,300,921 - DvMF_1873 0.16 -0.6 2,301,076 + DvMF_1873 0.28 +0.2 2,301,076 + DvMF_1873 0.28 +0.1 2,301,076 + DvMF_1873 0.28 -0.4 2,301,076 + DvMF_1873 0.28 +0.1 2,301,084 - DvMF_1873 0.28 +0.3 2,301,113 + DvMF_1873 0.31 +0.1 2,301,197 - DvMF_1873 0.37 -0.1 2,301,264 + DvMF_1873 0.42 +0.0
Or see this region's nucleotide sequence