Experiment: MoYLS4 with nitrite 1.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0013 and DvMF_0014 are separated by 654 nucleotides DvMF_0014 and DvMF_0015 are separated by 7 nucleotides DvMF_0015 and DvMF_0016 overlap by 4 nucleotides
DvMF_0013: DvMF_0013 - fructose-1,6-bisphosphate aldolase, class II (RefSeq), at 17,181 to 18,104
_0013
DvMF_0014: DvMF_0014 - hypothetical protein (RefSeq), at 18,759 to 21,203
_0014
DvMF_0015: DvMF_0015 - peptidase C26 (RefSeq), at 21,211 to 21,834
_0015
DvMF_0016: DvMF_0016 - transferase, putative (RefSeq), at 21,831 to 22,622
_0016
Position (kb) 18
19
20
21
22 Strain fitness (log2 ratio) -2
-1
0
1 at 18.305 kb on + strand at 18.576 kb on + strand at 18.707 kb on - strand at 18.715 kb on + strand at 18.810 kb on - strand at 18.817 kb on + strand at 19.087 kb on + strand, within DvMF_0014 at 19.227 kb on - strand, within DvMF_0014 at 19.329 kb on + strand, within DvMF_0014 at 19.369 kb on + strand, within DvMF_0014 at 19.369 kb on + strand, within DvMF_0014 at 19.369 kb on + strand, within DvMF_0014 at 19.369 kb on + strand, within DvMF_0014 at 19.377 kb on - strand, within DvMF_0014 at 19.377 kb on - strand, within DvMF_0014 at 19.469 kb on + strand, within DvMF_0014 at 20.010 kb on - strand, within DvMF_0014 at 20.116 kb on + strand, within DvMF_0014 at 20.276 kb on - strand, within DvMF_0014 at 21.162 kb on + strand at 21.197 kb on - strand at 21.205 kb on - strand at 21.241 kb on - strand at 21.285 kb on - strand, within DvMF_0015 at 21.836 kb on - strand at 22.011 kb on - strand, within DvMF_0016
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with nitrite 1.25 mg/ml remove 18,305 + -0.7 18,576 + -0.9 18,707 - -0.1 18,715 + -0.5 18,810 - -0.1 18,817 + -0.3 19,087 + DvMF_0014 0.13 -0.3 19,227 - DvMF_0014 0.19 -0.1 19,329 + DvMF_0014 0.23 -0.3 19,369 + DvMF_0014 0.25 -0.4 19,369 + DvMF_0014 0.25 -0.6 19,369 + DvMF_0014 0.25 -0.7 19,369 + DvMF_0014 0.25 -0.2 19,377 - DvMF_0014 0.25 +0.2 19,377 - DvMF_0014 0.25 -0.3 19,469 + DvMF_0014 0.29 -0.3 20,010 - DvMF_0014 0.51 +0.3 20,116 + DvMF_0014 0.56 +0.1 20,276 - DvMF_0014 0.62 +0.1 21,162 + -0.5 21,197 - -0.1 21,205 - -0.1 21,241 - -1.4 21,285 - DvMF_0015 0.12 -1.7 21,836 - -0.3 22,011 - DvMF_0016 0.23 -2.0
Or see this region's nucleotide sequence