Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2054 and Shewana3_2055 are separated by 18 nucleotides Shewana3_2055 and Shewana3_2056 are separated by 67 nucleotides Shewana3_2056 and Shewana3_2057 overlap by 7 nucleotides Shewana3_2057 and Shewana3_2058 overlap by 1 nucleotides Shewana3_2058 and Shewana3_2059 are separated by 14 nucleotides Shewana3_2059 and Shewana3_2060 are separated by 56 nucleotides
Shewana3_2054: Shewana3_2054 - seryl-tRNA synthetase (RefSeq), at 2,463,087 to 2,464,373
_2054
Shewana3_2055: Shewana3_2055 - hypothetical protein (RefSeq), at 2,464,392 to 2,464,514
_2055
Shewana3_2056: Shewana3_2056 - formate dehydrogenase (RefSeq), at 2,464,582 to 2,464,920
_2056
Shewana3_2057: Shewana3_2057 - DsrH family protein (RefSeq), at 2,464,914 to 2,465,195
_2057
Shewana3_2058: Shewana3_2058 - DsrE family protein (RefSeq), at 2,465,195 to 2,465,551
_2058
Shewana3_2059: Shewana3_2059 - DsrE family protein (RefSeq), at 2,465,566 to 2,465,958
_2059
Shewana3_2060: Shewana3_2060 - hypothetical protein (RefSeq), at 2,466,015 to 2,466,674
_2060
Position (kb)
2465
2466 Strain fitness (log2 ratio)
-2
-1
0
1 at 2464.382 kb on + strand at 2464.407 kb on + strand, within Shewana3_2055 at 2464.475 kb on + strand, within Shewana3_2055 at 2464.554 kb on - strand at 2466.070 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 2,464,382 + +1.4 2,464,407 + Shewana3_2055 0.12 +1.0 2,464,475 + Shewana3_2055 0.67 -1.9 2,464,554 - -2.7 2,466,070 - +0.5
Or see this region's nucleotide sequence