Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1730 and cysB are separated by 227 nucleotides cysB and Shewana3_1732 are separated by 81 nucleotides Shewana3_1732 and Shewana3_1733 are separated by 221 nucleotides
Shewana3_1730: Shewana3_1730 - hypothetical protein (RefSeq), at 2,038,597 to 2,039,376
_1730
Shewana3_1731: cysB - transcriptional regulator CysB (RefSeq), at 2,039,604 to 2,040,578
cysB
Shewana3_1732: Shewana3_1732 - two component LuxR family transcriptional regulator (RefSeq), at 2,040,660 to 2,041,322
_1732
Shewana3_1733: Shewana3_1733 - thioesterase superfamily protein (RefSeq), at 2,041,544 to 2,041,975
_1733
Position (kb)
2039
2040
2041 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2039.080 kb on + strand, within Shewana3_1730 at 2039.203 kb on - strand, within Shewana3_1730 at 2039.221 kb on - strand, within Shewana3_1730 at 2039.616 kb on - strand at 2039.618 kb on + strand at 2039.976 kb on + strand, within cysB at 2040.828 kb on + strand, within Shewana3_1732 at 2040.846 kb on - strand, within Shewana3_1732 at 2040.965 kb on - strand, within Shewana3_1732 at 2041.079 kb on + strand, within Shewana3_1732 at 2041.087 kb on - strand, within Shewana3_1732 at 2041.179 kb on + strand, within Shewana3_1732 at 2041.511 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 2,039,080 + Shewana3_1730 0.62 +1.1 2,039,203 - Shewana3_1730 0.78 +1.0 2,039,221 - Shewana3_1730 0.80 +0.4 2,039,616 - -2.7 2,039,618 + -3.3 2,039,976 + cysB Shewana3_1731 0.38 -2.3 2,040,828 + Shewana3_1732 0.25 -2.9 2,040,846 - Shewana3_1732 0.28 -0.3 2,040,965 - Shewana3_1732 0.46 -1.3 2,041,079 + Shewana3_1732 0.63 -3.5 2,041,087 - Shewana3_1732 0.64 -3.6 2,041,179 + Shewana3_1732 0.78 -2.3 2,041,511 - +0.2
Or see this region's nucleotide sequence