Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1178 and Shewana3_1179 are separated by 4 nucleotides Shewana3_1179 and Shewana3_1180 are separated by 84 nucleotides Shewana3_1180 and Shewana3_1181 are separated by 9 nucleotides Shewana3_1181 and trmB are separated by 179 nucleotides trmB and Shewana3_1183 are separated by 45 nucleotides
Shewana3_1178: Shewana3_1178 - cytochrome B561 (RefSeq), at 1,387,037 to 1,387,588
_1178
Shewana3_1179: Shewana3_1179 - hypothetical protein (RefSeq), at 1,387,593 to 1,388,168
_1179
Shewana3_1180: Shewana3_1180 - hypothetical protein (RefSeq), at 1,388,253 to 1,388,531
_1180
Shewana3_1181: Shewana3_1181 - A/G-specific DNA-adenine glycosylase (RefSeq), at 1,388,541 to 1,389,659
_1181
Shewana3_1182: trmB - tRNA (guanine-N(7)-)-methyltransferase (RefSeq), at 1,389,839 to 1,390,555
trmB
Shewana3_1183: Shewana3_1183 - hypothetical protein (RefSeq), at 1,390,601 to 1,390,927
_1183
Position (kb)
1388
1389
1390 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1387.558 kb on + strand at 1388.174 kb on + strand at 1388.250 kb on + strand at 1388.303 kb on - strand, within Shewana3_1180 at 1388.348 kb on + strand, within Shewana3_1180 at 1388.461 kb on + strand, within Shewana3_1180 at 1388.472 kb on - strand, within Shewana3_1180 at 1388.661 kb on + strand, within Shewana3_1181 at 1388.800 kb on + strand, within Shewana3_1181 at 1388.980 kb on + strand, within Shewana3_1181 at 1389.147 kb on - strand, within Shewana3_1181 at 1389.276 kb on + strand, within Shewana3_1181 at 1389.281 kb on + strand, within Shewana3_1181 at 1389.317 kb on + strand, within Shewana3_1181 at 1389.382 kb on + strand, within Shewana3_1181 at 1389.473 kb on - strand, within Shewana3_1181 at 1389.960 kb on + strand, within trmB at 1390.008 kb on + strand, within trmB
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 1,387,558 + -0.3 1,388,174 + -0.2 1,388,250 + +0.6 1,388,303 - Shewana3_1180 0.18 -0.7 1,388,348 + Shewana3_1180 0.34 -1.1 1,388,461 + Shewana3_1180 0.75 -2.9 1,388,472 - Shewana3_1180 0.78 -3.3 1,388,661 + Shewana3_1181 0.11 +0.5 1,388,800 + Shewana3_1181 0.23 +0.4 1,388,980 + Shewana3_1181 0.39 -0.9 1,389,147 - Shewana3_1181 0.54 +0.3 1,389,276 + Shewana3_1181 0.66 +1.1 1,389,281 + Shewana3_1181 0.66 +0.6 1,389,317 + Shewana3_1181 0.69 +1.3 1,389,382 + Shewana3_1181 0.75 +0.8 1,389,473 - Shewana3_1181 0.83 +0.3 1,389,960 + trmB Shewana3_1182 0.17 -0.3 1,390,008 + trmB Shewana3_1182 0.24 -0.5
Or see this region's nucleotide sequence