Experiment: D-Glucosamine Hydrochloride (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0617 and Shewana3_0618 are separated by 422 nucleotides Shewana3_0618 and Shewana3_0619 are separated by 120 nucleotides Shewana3_0619 and Shewana3_0620 are separated by 170 nucleotides
Shewana3_0617: Shewana3_0617 - cAMP-regulatory protein (RefSeq), at 722,231 to 722,866
_0617
Shewana3_0618: Shewana3_0618 - Sel1 domain-containing protein (RefSeq), at 723,289 to 724,068
_0618
Shewana3_0619: Shewana3_0619 - 2'-5' RNA ligase (RefSeq), at 724,189 to 724,761
_0619
Shewana3_0620: Shewana3_0620 - diguanylate cyclase/phosphodiesterase (RefSeq), at 724,932 to 727,118
_0620
Position (kb) 723
724
725 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 722.711 kb on - strand, within Shewana3_0617 at 723.001 kb on - strand at 723.059 kb on + strand at 723.065 kb on + strand at 723.065 kb on + strand at 723.073 kb on + strand at 723.073 kb on + strand at 723.073 kb on + strand at 723.073 kb on - strand at 723.073 kb on - strand at 723.327 kb on - strand at 723.339 kb on + strand at 723.344 kb on + strand at 723.578 kb on + strand, within Shewana3_0618 at 723.641 kb on - strand, within Shewana3_0618 at 723.709 kb on + strand, within Shewana3_0618 at 724.180 kb on + strand at 724.266 kb on + strand, within Shewana3_0619 at 724.266 kb on + strand, within Shewana3_0619 at 724.291 kb on + strand, within Shewana3_0619 at 724.326 kb on + strand, within Shewana3_0619 at 724.334 kb on - strand, within Shewana3_0619 at 724.334 kb on - strand, within Shewana3_0619 at 724.441 kb on - strand, within Shewana3_0619 at 724.517 kb on - strand, within Shewana3_0619 at 724.724 kb on - strand at 724.738 kb on + strand at 725.041 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucosamine Hydrochloride (C) remove 722,711 - Shewana3_0617 0.75 +0.2 723,001 - +0.3 723,059 + -0.7 723,065 + -2.0 723,065 + -1.9 723,073 + +0.7 723,073 + +0.4 723,073 + -1.1 723,073 - -0.6 723,073 - +0.6 723,327 - -1.4 723,339 + +0.3 723,344 + +1.5 723,578 + Shewana3_0618 0.37 -3.2 723,641 - Shewana3_0618 0.45 -0.0 723,709 + Shewana3_0618 0.54 +0.1 724,180 + -0.0 724,266 + Shewana3_0619 0.13 +0.4 724,266 + Shewana3_0619 0.13 -0.9 724,291 + Shewana3_0619 0.18 -0.8 724,326 + Shewana3_0619 0.24 +0.2 724,334 - Shewana3_0619 0.25 -1.7 724,334 - Shewana3_0619 0.25 -0.4 724,441 - Shewana3_0619 0.44 -2.2 724,517 - Shewana3_0619 0.57 -0.5 724,724 - +0.6 724,738 + +0.6 725,041 - -1.7
Or see this region's nucleotide sequence