Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4530

Experiment: D-Galactose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4529 and Pf6N2E2_4530 overlap by 4 nucleotidesPf6N2E2_4530 and Pf6N2E2_4531 are separated by 157 nucleotides Pf6N2E2_4529 - type III secretion protein HrpJ, at 5,262,731 to 5,263,858 _4529 Pf6N2E2_4530 - Type III secretion inner membrane channel protein (LcrD,HrcV,EscV,SsaV), at 5,263,855 to 5,265,942 _4530 Pf6N2E2_4531 - hypothetical protein, at 5,266,100 to 5,266,993 _4531 Position (kb) 5263 5264 5265 5266Strain fitness (log2 ratio) -1 0 1 2 3at 5262.892 kb on + strand, within Pf6N2E2_4529at 5262.893 kb on - strand, within Pf6N2E2_4529at 5262.893 kb on - strand, within Pf6N2E2_4529at 5263.197 kb on + strandat 5263.198 kb on - strand, within Pf6N2E2_4529at 5263.198 kb on - strand, within Pf6N2E2_4529at 5263.198 kb on - strand, within Pf6N2E2_4529at 5263.304 kb on + strand, within Pf6N2E2_4529at 5263.304 kb on + strand, within Pf6N2E2_4529at 5263.304 kb on + strand, within Pf6N2E2_4529at 5263.305 kb on - strand, within Pf6N2E2_4529at 5263.307 kb on + strand, within Pf6N2E2_4529at 5263.307 kb on + strand, within Pf6N2E2_4529at 5263.308 kb on - strand, within Pf6N2E2_4529at 5263.308 kb on - strand, within Pf6N2E2_4529at 5263.308 kb on - strand, within Pf6N2E2_4529at 5263.552 kb on + strand, within Pf6N2E2_4529at 5263.552 kb on + strand, within Pf6N2E2_4529at 5263.638 kb on + strand, within Pf6N2E2_4529at 5263.638 kb on + strand, within Pf6N2E2_4529at 5263.639 kb on - strand, within Pf6N2E2_4529at 5263.645 kb on + strand, within Pf6N2E2_4529at 5263.646 kb on - strand, within Pf6N2E2_4529at 5263.706 kb on + strand, within Pf6N2E2_4529at 5264.032 kb on + strandat 5264.033 kb on - strandat 5264.551 kb on + strand, within Pf6N2E2_4530at 5264.552 kb on - strand, within Pf6N2E2_4530at 5264.552 kb on - strand, within Pf6N2E2_4530at 5264.552 kb on - strand, within Pf6N2E2_4530at 5264.613 kb on + strand, within Pf6N2E2_4530at 5264.614 kb on - strand, within Pf6N2E2_4530at 5264.828 kb on - strand, within Pf6N2E2_4530at 5264.933 kb on - strand, within Pf6N2E2_4530at 5265.010 kb on - strand, within Pf6N2E2_4530at 5265.062 kb on - strand, within Pf6N2E2_4530at 5265.062 kb on - strand, within Pf6N2E2_4530at 5265.587 kb on - strand, within Pf6N2E2_4530at 5265.587 kb on - strand, within Pf6N2E2_4530at 5265.587 kb on - strand, within Pf6N2E2_4530at 5265.981 kb on + strandat 5266.053 kb on - strandat 5266.053 kb on - strandat 5266.206 kb on - strand, within Pf6N2E2_4531

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Galactose (C)
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5,262,892 + Pf6N2E2_4529 0.14 -0.3
5,262,893 - Pf6N2E2_4529 0.14 +1.7
5,262,893 - Pf6N2E2_4529 0.14 +0.5
5,263,197 + +0.8
5,263,198 - Pf6N2E2_4529 0.41 +0.7
5,263,198 - Pf6N2E2_4529 0.41 +0.3
5,263,198 - Pf6N2E2_4529 0.41 -0.6
5,263,304 + Pf6N2E2_4529 0.51 -0.2
5,263,304 + Pf6N2E2_4529 0.51 +0.9
5,263,304 + Pf6N2E2_4529 0.51 +0.1
5,263,305 - Pf6N2E2_4529 0.51 -0.2
5,263,307 + Pf6N2E2_4529 0.51 -1.2
5,263,307 + Pf6N2E2_4529 0.51 -0.4
5,263,308 - Pf6N2E2_4529 0.51 -0.3
5,263,308 - Pf6N2E2_4529 0.51 -0.3
5,263,308 - Pf6N2E2_4529 0.51 +0.3
5,263,552 + Pf6N2E2_4529 0.73 +0.2
5,263,552 + Pf6N2E2_4529 0.73 +0.5
5,263,638 + Pf6N2E2_4529 0.80 -0.0
5,263,638 + Pf6N2E2_4529 0.80 +1.3
5,263,639 - Pf6N2E2_4529 0.80 +0.6
5,263,645 + Pf6N2E2_4529 0.81 -0.4
5,263,646 - Pf6N2E2_4529 0.81 -0.3
5,263,706 + Pf6N2E2_4529 0.86 +0.2
5,264,032 + +0.8
5,264,033 - -0.1
5,264,551 + Pf6N2E2_4530 0.33 -0.2
5,264,552 - Pf6N2E2_4530 0.33 +1.0
5,264,552 - Pf6N2E2_4530 0.33 -1.4
5,264,552 - Pf6N2E2_4530 0.33 +3.6
5,264,613 + Pf6N2E2_4530 0.36 +0.9
5,264,614 - Pf6N2E2_4530 0.36 +0.9
5,264,828 - Pf6N2E2_4530 0.47 +0.3
5,264,933 - Pf6N2E2_4530 0.52 -0.1
5,265,010 - Pf6N2E2_4530 0.55 +0.0
5,265,062 - Pf6N2E2_4530 0.58 +0.6
5,265,062 - Pf6N2E2_4530 0.58 +1.2
5,265,587 - Pf6N2E2_4530 0.83 +0.5
5,265,587 - Pf6N2E2_4530 0.83 -1.6
5,265,587 - Pf6N2E2_4530 0.83 +0.9
5,265,981 + -1.3
5,266,053 - +0.3
5,266,053 - +1.3
5,266,206 - Pf6N2E2_4531 0.12 +2.8

Or see this region's nucleotide sequence