Experiment: D-Galactose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_136 and Pf6N2E2_137 overlap by 1 nucleotides Pf6N2E2_137 and Pf6N2E2_138 are separated by 30 nucleotides
Pf6N2E2_136 - Glycolate dehydrogenase (EC 1.1.99.14), FAD-binding subunit GlcE, at 185,041 to 186,099
_136
Pf6N2E2_137 - Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD, at 186,099 to 187,634
_137
Pf6N2E2_138 - Alpha/beta hydrolase fold (EC 3.8.1.5), at 187,665 to 188,594
_138
Position (kb) 186
187
188 Strain fitness (log2 ratio) -1
0
1
2 at 185.114 kb on + strand at 185.376 kb on - strand, within Pf6N2E2_136 at 185.557 kb on - strand, within Pf6N2E2_136 at 185.557 kb on - strand, within Pf6N2E2_136 at 185.928 kb on - strand, within Pf6N2E2_136 at 186.545 kb on - strand, within Pf6N2E2_137 at 186.547 kb on + strand, within Pf6N2E2_137 at 186.547 kb on + strand, within Pf6N2E2_137 at 186.547 kb on + strand, within Pf6N2E2_137 at 186.547 kb on + strand, within Pf6N2E2_137 at 186.548 kb on - strand, within Pf6N2E2_137 at 186.548 kb on - strand, within Pf6N2E2_137 at 186.548 kb on - strand, within Pf6N2E2_137 at 187.085 kb on - strand, within Pf6N2E2_137 at 187.168 kb on + strand, within Pf6N2E2_137 at 187.168 kb on + strand, within Pf6N2E2_137 at 187.168 kb on + strand, within Pf6N2E2_137 at 187.171 kb on + strand, within Pf6N2E2_137 at 187.171 kb on + strand, within Pf6N2E2_137 at 187.172 kb on - strand, within Pf6N2E2_137 at 187.293 kb on + strand, within Pf6N2E2_137 at 187.293 kb on + strand, within Pf6N2E2_137 at 187.294 kb on - strand, within Pf6N2E2_137 at 187.359 kb on + strand, within Pf6N2E2_137 at 187.360 kb on - strand, within Pf6N2E2_137 at 187.468 kb on + strand, within Pf6N2E2_137 at 187.585 kb on + strand at 187.585 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.635 kb on - strand at 187.635 kb on - strand at 187.635 kb on - strand at 187.635 kb on - strand at 187.635 kb on - strand at 187.635 kb on - strand at 187.635 kb on - strand at 187.840 kb on - strand, within Pf6N2E2_138 at 187.840 kb on - strand, within Pf6N2E2_138 at 187.840 kb on - strand, within Pf6N2E2_138 at 188.005 kb on + strand, within Pf6N2E2_138 at 188.006 kb on - strand, within Pf6N2E2_138 at 188.171 kb on - strand, within Pf6N2E2_138 at 188.263 kb on + strand, within Pf6N2E2_138 at 188.326 kb on + strand, within Pf6N2E2_138 at 188.381 kb on - strand, within Pf6N2E2_138 at 188.515 kb on + strand at 188.540 kb on - strand at 188.540 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Galactose (C) remove 185,114 + +0.1 185,376 - Pf6N2E2_136 0.32 +0.2 185,557 - Pf6N2E2_136 0.49 -0.4 185,557 - Pf6N2E2_136 0.49 +0.2 185,928 - Pf6N2E2_136 0.84 -0.3 186,545 - Pf6N2E2_137 0.29 +0.3 186,547 + Pf6N2E2_137 0.29 +0.7 186,547 + Pf6N2E2_137 0.29 +1.4 186,547 + Pf6N2E2_137 0.29 -0.4 186,547 + Pf6N2E2_137 0.29 -1.5 186,548 - Pf6N2E2_137 0.29 +1.0 186,548 - Pf6N2E2_137 0.29 +0.1 186,548 - Pf6N2E2_137 0.29 -0.2 187,085 - Pf6N2E2_137 0.64 -0.3 187,168 + Pf6N2E2_137 0.70 +0.3 187,168 + Pf6N2E2_137 0.70 +0.6 187,168 + Pf6N2E2_137 0.70 -0.1 187,171 + Pf6N2E2_137 0.70 -0.1 187,171 + Pf6N2E2_137 0.70 +0.9 187,172 - Pf6N2E2_137 0.70 -0.8 187,293 + Pf6N2E2_137 0.78 +1.1 187,293 + Pf6N2E2_137 0.78 -0.0 187,294 - Pf6N2E2_137 0.78 +0.3 187,359 + Pf6N2E2_137 0.82 +2.7 187,360 - Pf6N2E2_137 0.82 -0.3 187,468 + Pf6N2E2_137 0.89 -0.8 187,585 + +0.8 187,585 + -0.6 187,634 + +0.5 187,634 + +0.9 187,634 + +0.3 187,634 + +1.5 187,634 + +1.2 187,634 + -0.5 187,634 + +0.4 187,634 + +0.3 187,634 + +0.4 187,634 + +0.7 187,634 + -1.0 187,635 - +0.5 187,635 - +0.5 187,635 - +0.0 187,635 - -0.1 187,635 - -0.8 187,635 - -0.0 187,635 - -0.4 187,840 - Pf6N2E2_138 0.19 +0.4 187,840 - Pf6N2E2_138 0.19 -0.3 187,840 - Pf6N2E2_138 0.19 -1.6 188,005 + Pf6N2E2_138 0.37 +1.3 188,006 - Pf6N2E2_138 0.37 +0.0 188,171 - Pf6N2E2_138 0.54 +0.9 188,263 + Pf6N2E2_138 0.64 -0.5 188,326 + Pf6N2E2_138 0.71 +1.2 188,381 - Pf6N2E2_138 0.77 +0.2 188,515 + +0.5 188,540 - +0.4 188,540 - +1.4
Or see this region's nucleotide sequence