Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS09045 and MMJJ_RS09050 are separated by 114 nucleotides MMJJ_RS09050 and MMJJ_RS09055 are separated by 87 nucleotides MMJJ_RS09055 and MMJJ_RS09060 are separated by 296 nucleotides MMJJ_RS09060 and MMJJ_RS09065 are separated by 230 nucleotides
MMJJ_RS09045: MMJJ_RS09045 - EMC3/TMCO1 family protein, at 1,671,100 to 1,671,696
_RS09045
MMJJ_RS09050: MMJJ_RS09050 - hypothetical protein, at 1,671,811 to 1,672,170
_RS09050
MMJJ_RS09055: MMJJ_RS09055 - NFACT family protein, at 1,672,258 to 1,674,300
_RS09055
MMJJ_RS09060: MMJJ_RS09060 - hypothetical protein, at 1,674,597 to 1,674,824
_RS09060
MMJJ_RS09065: MMJJ_RS09065 - hypothetical protein, at 1,675,055 to 1,675,606
_RS09065
Position (kb)
1672
1673
1674
1675 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1671.513 kb on + strand, within MMJJ_RS09045 at 1671.672 kb on - strand at 1671.673 kb on + strand at 1671.673 kb on + strand at 1671.681 kb on + strand at 1671.713 kb on + strand at 1671.717 kb on - strand at 1671.717 kb on - strand at 1671.717 kb on - strand at 1671.718 kb on + strand at 1671.718 kb on + strand at 1671.728 kb on - strand at 1671.755 kb on + strand at 1671.760 kb on + strand at 1671.767 kb on + strand at 1671.788 kb on + strand at 1671.788 kb on + strand at 1671.819 kb on + strand at 1671.867 kb on - strand, within MMJJ_RS09050 at 1671.945 kb on - strand, within MMJJ_RS09050 at 1671.991 kb on + strand, within MMJJ_RS09050 at 1672.052 kb on - strand, within MMJJ_RS09050 at 1672.053 kb on + strand, within MMJJ_RS09050 at 1672.138 kb on - strand at 1672.139 kb on + strand at 1672.162 kb on + strand at 1672.187 kb on + strand at 1672.197 kb on - strand at 1672.305 kb on - strand at 1672.388 kb on + strand at 1672.390 kb on - strand at 1672.437 kb on - strand at 1672.437 kb on - strand at 1672.437 kb on - strand at 1672.437 kb on - strand at 1672.441 kb on - strand at 1672.442 kb on + strand at 1672.547 kb on - strand, within MMJJ_RS09055 at 1672.548 kb on + strand, within MMJJ_RS09055 at 1672.684 kb on - strand, within MMJJ_RS09055 at 1672.707 kb on - strand, within MMJJ_RS09055 at 1672.707 kb on - strand, within MMJJ_RS09055 at 1672.717 kb on + strand, within MMJJ_RS09055 at 1672.750 kb on - strand, within MMJJ_RS09055 at 1672.750 kb on - strand, within MMJJ_RS09055 at 1672.750 kb on - strand, within MMJJ_RS09055 at 1672.750 kb on - strand, within MMJJ_RS09055 at 1672.751 kb on + strand, within MMJJ_RS09055 at 1672.769 kb on - strand, within MMJJ_RS09055 at 1672.820 kb on + strand, within MMJJ_RS09055 at 1672.942 kb on - strand, within MMJJ_RS09055 at 1672.973 kb on + strand, within MMJJ_RS09055 at 1673.029 kb on - strand, within MMJJ_RS09055 at 1673.030 kb on + strand, within MMJJ_RS09055 at 1673.030 kb on + strand, within MMJJ_RS09055 at 1673.031 kb on - strand, within MMJJ_RS09055 at 1673.031 kb on - strand, within MMJJ_RS09055 at 1673.246 kb on + strand, within MMJJ_RS09055 at 1673.257 kb on - strand, within MMJJ_RS09055 at 1673.258 kb on + strand at 1673.356 kb on + strand, within MMJJ_RS09055 at 1673.403 kb on - strand, within MMJJ_RS09055 at 1673.564 kb on + strand, within MMJJ_RS09055 at 1673.564 kb on + strand, within MMJJ_RS09055 at 1673.723 kb on + strand, within MMJJ_RS09055 at 1673.917 kb on - strand, within MMJJ_RS09055 at 1673.917 kb on - strand, within MMJJ_RS09055 at 1673.938 kb on - strand, within MMJJ_RS09055 at 1674.022 kb on + strand, within MMJJ_RS09055 at 1674.051 kb on - strand, within MMJJ_RS09055 at 1674.113 kb on + strand at 1674.117 kb on + strand at 1674.117 kb on + strand at 1674.173 kb on + strand at 1674.346 kb on - strand at 1674.346 kb on - strand at 1674.347 kb on + strand at 1674.347 kb on + strand at 1674.350 kb on - strand at 1674.350 kb on - strand at 1674.355 kb on + strand at 1674.355 kb on + strand at 1674.361 kb on - strand at 1674.361 kb on - strand at 1674.361 kb on - strand at 1674.361 kb on - strand at 1674.362 kb on + strand at 1674.362 kb on + strand at 1674.370 kb on - strand at 1674.370 kb on - strand at 1674.379 kb on + strand at 1674.381 kb on - strand at 1674.381 kb on - strand at 1674.389 kb on - strand at 1674.389 kb on - strand at 1674.389 kb on - strand at 1674.390 kb on + strand at 1674.390 kb on + strand at 1674.390 kb on + strand at 1674.401 kb on - strand at 1674.410 kb on + strand at 1674.417 kb on - strand at 1674.418 kb on + strand at 1674.419 kb on - strand at 1674.419 kb on - strand at 1674.420 kb on + strand at 1674.420 kb on + strand at 1674.420 kb on + strand at 1674.422 kb on + strand at 1674.431 kb on + strand at 1674.432 kb on - strand at 1674.433 kb on + strand at 1674.433 kb on + strand at 1674.435 kb on - strand at 1674.435 kb on - strand at 1674.435 kb on - strand at 1674.435 kb on - strand at 1674.435 kb on - strand at 1674.436 kb on + strand at 1674.436 kb on + strand at 1674.436 kb on + strand at 1674.440 kb on - strand at 1674.441 kb on + strand at 1674.441 kb on + strand at 1674.442 kb on - strand at 1674.442 kb on - strand at 1674.443 kb on + strand at 1674.443 kb on + strand at 1674.443 kb on + strand at 1674.443 kb on + strand at 1674.443 kb on + strand at 1674.446 kb on - strand at 1674.446 kb on - strand at 1674.446 kb on - strand at 1674.446 kb on - strand at 1674.446 kb on - strand at 1674.446 kb on - strand at 1674.446 kb on - strand at 1674.447 kb on + strand at 1674.447 kb on + strand at 1674.447 kb on + strand at 1674.487 kb on - strand at 1674.492 kb on + strand at 1674.492 kb on + strand at 1674.521 kb on + strand at 1674.537 kb on - strand at 1674.540 kb on + strand at 1674.547 kb on + strand at 1674.547 kb on + strand at 1674.592 kb on - strand at 1674.593 kb on + strand at 1674.605 kb on - strand at 1674.631 kb on + strand, within MMJJ_RS09060 at 1674.643 kb on + strand, within MMJJ_RS09060 at 1674.657 kb on - strand, within MMJJ_RS09060 at 1674.657 kb on - strand, within MMJJ_RS09060 at 1674.678 kb on - strand, within MMJJ_RS09060 at 1674.687 kb on - strand, within MMJJ_RS09060 at 1674.687 kb on - strand, within MMJJ_RS09060 at 1674.687 kb on - strand, within MMJJ_RS09060 at 1674.688 kb on + strand, within MMJJ_RS09060 at 1674.688 kb on + strand, within MMJJ_RS09060 at 1674.688 kb on + strand, within MMJJ_RS09060 at 1674.689 kb on - strand, within MMJJ_RS09060 at 1674.689 kb on - strand, within MMJJ_RS09060 at 1674.689 kb on - strand, within MMJJ_RS09060 at 1674.689 kb on - strand, within MMJJ_RS09060 at 1674.689 kb on - strand, within MMJJ_RS09060 at 1674.690 kb on + strand, within MMJJ_RS09060 at 1674.690 kb on + strand, within MMJJ_RS09060 at 1674.690 kb on + strand, within MMJJ_RS09060 at 1674.690 kb on + strand, within MMJJ_RS09060 at 1674.690 kb on + strand, within MMJJ_RS09060 at 1674.690 kb on + strand, within MMJJ_RS09060 at 1674.690 kb on + strand, within MMJJ_RS09060 at 1674.691 kb on - strand, within MMJJ_RS09060 at 1674.691 kb on - strand, within MMJJ_RS09060 at 1674.691 kb on - strand, within MMJJ_RS09060 at 1674.692 kb on + strand, within MMJJ_RS09060 at 1674.692 kb on + strand, within MMJJ_RS09060 at 1674.692 kb on + strand, within MMJJ_RS09060 at 1674.732 kb on - strand, within MMJJ_RS09060 at 1674.754 kb on + strand, within MMJJ_RS09060 at 1674.775 kb on - strand, within MMJJ_RS09060 at 1674.827 kb on - strand at 1674.828 kb on + strand at 1674.867 kb on + strand at 1674.877 kb on + strand at 1674.878 kb on - strand at 1674.917 kb on - strand at 1674.957 kb on + strand at 1674.983 kb on - strand at 1674.983 kb on - strand at 1674.983 kb on - strand at 1674.984 kb on + strand at 1674.985 kb on - strand at 1674.986 kb on + strand at 1674.997 kb on - strand at 1674.997 kb on - strand at 1675.001 kb on - strand at 1675.001 kb on - strand at 1675.001 kb on - strand at 1675.001 kb on - strand at 1675.001 kb on - strand at 1675.001 kb on - strand at 1675.002 kb on + strand at 1675.002 kb on + strand at 1675.002 kb on + strand at 1675.017 kb on - strand at 1675.017 kb on - strand at 1675.021 kb on - strand at 1675.021 kb on - strand at 1675.081 kb on - strand at 1675.098 kb on + strand at 1675.145 kb on + strand, within MMJJ_RS09065 at 1675.153 kb on + strand, within MMJJ_RS09065 at 1675.153 kb on + strand, within MMJJ_RS09065 at 1675.159 kb on - strand, within MMJJ_RS09065 at 1675.235 kb on + strand, within MMJJ_RS09065 at 1675.235 kb on + strand, within MMJJ_RS09065 at 1675.253 kb on - strand, within MMJJ_RS09065
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE remove 1,671,513 + MMJJ_RS09045 0.69 -1.7 1,671,672 - +0.8 1,671,673 + +0.2 1,671,673 + -0.5 1,671,681 + -0.5 1,671,713 + -0.4 1,671,717 - +0.1 1,671,717 - +1.4 1,671,717 - +0.7 1,671,718 + -0.6 1,671,718 + -0.1 1,671,728 - +0.7 1,671,755 + -0.7 1,671,760 + +0.1 1,671,767 + +0.1 1,671,788 + +0.1 1,671,788 + +0.1 1,671,819 + +0.7 1,671,867 - MMJJ_RS09050 0.16 -0.2 1,671,945 - MMJJ_RS09050 0.37 +0.4 1,671,991 + MMJJ_RS09050 0.50 +0.1 1,672,052 - MMJJ_RS09050 0.67 +0.1 1,672,053 + MMJJ_RS09050 0.67 +0.4 1,672,138 - +0.2 1,672,139 + -0.9 1,672,162 + -0.0 1,672,187 + -0.0 1,672,197 - -0.2 1,672,305 - -1.7 1,672,388 + -1.2 1,672,390 - -0.5 1,672,437 - -2.2 1,672,437 - -1.8 1,672,437 - -2.1 1,672,437 - -1.7 1,672,441 - -4.1 1,672,442 + -2.9 1,672,547 - MMJJ_RS09055 0.14 -1.6 1,672,548 + MMJJ_RS09055 0.14 -2.5 1,672,684 - MMJJ_RS09055 0.21 -3.0 1,672,707 - MMJJ_RS09055 0.22 -2.7 1,672,707 - MMJJ_RS09055 0.22 -1.0 1,672,717 + MMJJ_RS09055 0.22 +0.8 1,672,750 - MMJJ_RS09055 0.24 -1.8 1,672,750 - MMJJ_RS09055 0.24 -2.4 1,672,750 - MMJJ_RS09055 0.24 -1.4 1,672,750 - MMJJ_RS09055 0.24 +0.5 1,672,751 + MMJJ_RS09055 0.24 -2.7 1,672,769 - MMJJ_RS09055 0.25 -2.9 1,672,820 + MMJJ_RS09055 0.28 +0.3 1,672,942 - MMJJ_RS09055 0.33 -2.2 1,672,973 + MMJJ_RS09055 0.35 -1.9 1,673,029 - MMJJ_RS09055 0.38 -1.2 1,673,030 + MMJJ_RS09055 0.38 +0.2 1,673,030 + MMJJ_RS09055 0.38 -0.7 1,673,031 - MMJJ_RS09055 0.38 -3.3 1,673,031 - MMJJ_RS09055 0.38 -1.9 1,673,246 + MMJJ_RS09055 0.48 +1.2 1,673,257 - MMJJ_RS09055 0.49 -1.4 1,673,258 + -1.2 1,673,356 + MMJJ_RS09055 0.54 -0.9 1,673,403 - MMJJ_RS09055 0.56 -1.0 1,673,564 + MMJJ_RS09055 0.64 -2.6 1,673,564 + MMJJ_RS09055 0.64 -2.5 1,673,723 + MMJJ_RS09055 0.72 -0.2 1,673,917 - MMJJ_RS09055 0.81 +0.6 1,673,917 - MMJJ_RS09055 0.81 -1.2 1,673,938 - MMJJ_RS09055 0.82 -0.7 1,674,022 + MMJJ_RS09055 0.86 -1.6 1,674,051 - MMJJ_RS09055 0.88 -1.2 1,674,113 + -3.0 1,674,117 + -1.6 1,674,117 + -3.7 1,674,173 + +0.8 1,674,346 - +0.0 1,674,346 - -0.2 1,674,347 + -0.7 1,674,347 + +0.8 1,674,350 - -1.3 1,674,350 - +0.4 1,674,355 + +0.6 1,674,355 + +0.0 1,674,361 - -0.4 1,674,361 - +0.1 1,674,361 - -1.1 1,674,361 - -0.3 1,674,362 + +0.4 1,674,362 + +0.1 1,674,370 - -0.0 1,674,370 - +1.8 1,674,379 + +0.5 1,674,381 - -0.0 1,674,381 - -0.2 1,674,389 - -0.3 1,674,389 - +1.3 1,674,389 - +0.0 1,674,390 + +0.3 1,674,390 + -0.8 1,674,390 + +0.8 1,674,401 - +0.3 1,674,410 + -0.2 1,674,417 - +0.1 1,674,418 + -1.1 1,674,419 - +0.3 1,674,419 - -0.3 1,674,420 + +0.5 1,674,420 + +0.4 1,674,420 + +0.5 1,674,422 + -0.2 1,674,431 + +0.0 1,674,432 - -0.2 1,674,433 + +0.2 1,674,433 + -0.4 1,674,435 - -0.0 1,674,435 - +0.2 1,674,435 - -1.2 1,674,435 - +0.9 1,674,435 - -0.9 1,674,436 + -0.2 1,674,436 + -0.5 1,674,436 + +0.4 1,674,440 - -0.4 1,674,441 + +0.3 1,674,441 + -1.0 1,674,442 - +0.7 1,674,442 - -0.2 1,674,443 + +0.1 1,674,443 + +0.3 1,674,443 + +0.1 1,674,443 + -0.2 1,674,443 + +0.6 1,674,446 - +0.8 1,674,446 - -0.1 1,674,446 - +0.0 1,674,446 - -0.7 1,674,446 - +0.8 1,674,446 - +0.7 1,674,446 - +0.7 1,674,447 + +0.2 1,674,447 + +0.4 1,674,447 + +0.4 1,674,487 - +0.2 1,674,492 + +0.7 1,674,492 + -0.4 1,674,521 + -0.1 1,674,537 - -0.8 1,674,540 + +0.5 1,674,547 + +0.9 1,674,547 + -0.7 1,674,592 - -0.2 1,674,593 + +0.6 1,674,605 - +0.2 1,674,631 + MMJJ_RS09060 0.15 -1.0 1,674,643 + MMJJ_RS09060 0.20 +0.8 1,674,657 - MMJJ_RS09060 0.26 +0.0 1,674,657 - MMJJ_RS09060 0.26 +0.4 1,674,678 - MMJJ_RS09060 0.36 +0.4 1,674,687 - MMJJ_RS09060 0.39 +0.1 1,674,687 - MMJJ_RS09060 0.39 -0.1 1,674,687 - MMJJ_RS09060 0.39 -0.2 1,674,688 + MMJJ_RS09060 0.40 -0.4 1,674,688 + MMJJ_RS09060 0.40 +0.2 1,674,688 + MMJJ_RS09060 0.40 +0.4 1,674,689 - MMJJ_RS09060 0.40 +0.0 1,674,689 - MMJJ_RS09060 0.40 +0.4 1,674,689 - MMJJ_RS09060 0.40 -0.1 1,674,689 - MMJJ_RS09060 0.40 +0.8 1,674,689 - MMJJ_RS09060 0.40 -0.7 1,674,690 + MMJJ_RS09060 0.41 -0.8 1,674,690 + MMJJ_RS09060 0.41 -0.2 1,674,690 + MMJJ_RS09060 0.41 -0.2 1,674,690 + MMJJ_RS09060 0.41 +1.4 1,674,690 + MMJJ_RS09060 0.41 -0.2 1,674,690 + MMJJ_RS09060 0.41 +0.8 1,674,690 + MMJJ_RS09060 0.41 -0.7 1,674,691 - MMJJ_RS09060 0.41 -0.3 1,674,691 - MMJJ_RS09060 0.41 +0.1 1,674,691 - MMJJ_RS09060 0.41 +0.1 1,674,692 + MMJJ_RS09060 0.42 -0.4 1,674,692 + MMJJ_RS09060 0.42 +0.5 1,674,692 + MMJJ_RS09060 0.42 +0.0 1,674,732 - MMJJ_RS09060 0.59 +0.4 1,674,754 + MMJJ_RS09060 0.69 +0.1 1,674,775 - MMJJ_RS09060 0.78 +0.6 1,674,827 - -0.7 1,674,828 + +0.7 1,674,867 + +1.2 1,674,877 + +0.4 1,674,878 - +0.2 1,674,917 - +0.7 1,674,957 + +0.5 1,674,983 - +0.2 1,674,983 - -1.7 1,674,983 - -0.2 1,674,984 + -0.6 1,674,985 - +0.2 1,674,986 + -0.3 1,674,997 - +0.7 1,674,997 - -0.1 1,675,001 - +0.7 1,675,001 - -0.3 1,675,001 - +0.7 1,675,001 - +0.2 1,675,001 - -0.5 1,675,001 - -0.9 1,675,002 + -0.3 1,675,002 + -0.6 1,675,002 + +0.5 1,675,017 - -1.0 1,675,017 - +0.6 1,675,021 - -1.1 1,675,021 - -0.6 1,675,081 - +1.1 1,675,098 + +0.1 1,675,145 + MMJJ_RS09065 0.16 -0.2 1,675,153 + MMJJ_RS09065 0.18 +0.1 1,675,153 + MMJJ_RS09065 0.18 +0.1 1,675,159 - MMJJ_RS09065 0.19 -0.3 1,675,235 + MMJJ_RS09065 0.33 +0.1 1,675,235 + MMJJ_RS09065 0.33 +0.1 1,675,253 - MMJJ_RS09065 0.36 -0.0
Or see this region's nucleotide sequence