Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS03125

Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS03120 and MMJJ_RS03125 are separated by 131 nucleotidesMMJJ_RS03125 and MMJJ_RS03130 are separated by 21 nucleotides MMJJ_RS03120: MMJJ_RS03120 - ribose-phosphate diphosphokinase, at 589,229 to 590,092 _RS03120 MMJJ_RS03125: MMJJ_RS03125 - GMC oxidoreductase, at 590,224 to 591,384 _RS03125 MMJJ_RS03130: MMJJ_RS03130 - DUF1611 domain-containing protein, at 591,406 to 592,326 _RS03130 Position (kb) 590 591 592Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 589.226 kb on - strandat 589.227 kb on + strandat 589.230 kb on - strandat 589.241 kb on - strandat 589.244 kb on - strandat 589.244 kb on - strandat 589.244 kb on - strandat 589.244 kb on - strandat 589.245 kb on + strandat 589.272 kb on + strandat 589.485 kb on + strandat 589.485 kb on + strandat 589.844 kb on - strand, within MMJJ_RS03120at 590.126 kb on - strandat 590.147 kb on - strandat 590.147 kb on - strandat 590.165 kb on - strandat 590.181 kb on - strandat 590.244 kb on + strandat 590.265 kb on - strandat 590.310 kb on + strandat 590.310 kb on + strandat 590.310 kb on + strandat 590.341 kb on - strand, within MMJJ_RS03125at 590.468 kb on - strand, within MMJJ_RS03125at 590.469 kb on + strandat 590.469 kb on + strand, within MMJJ_RS03125at 590.470 kb on - strand, within MMJJ_RS03125at 590.706 kb on + strandat 590.736 kb on + strand, within MMJJ_RS03125at 590.763 kb on - strand, within MMJJ_RS03125at 590.770 kb on - strand, within MMJJ_RS03125at 590.834 kb on - strand, within MMJJ_RS03125at 590.835 kb on + strand, within MMJJ_RS03125at 590.838 kb on + strand, within MMJJ_RS03125at 590.888 kb on - strand, within MMJJ_RS03125at 591.283 kb on - strandat 591.342 kb on - strandat 591.439 kb on - strandat 591.439 kb on - strandat 591.440 kb on + strandat 591.482 kb on + strandat 591.580 kb on - strand, within MMJJ_RS03130at 591.580 kb on - strand, within MMJJ_RS03130at 591.760 kb on - strand, within MMJJ_RS03130at 591.903 kb on - strand, within MMJJ_RS03130at 591.985 kb on - strand, within MMJJ_RS03130at 591.994 kb on + strand, within MMJJ_RS03130at 592.022 kb on - strand, within MMJJ_RS03130at 592.022 kb on - strand, within MMJJ_RS03130at 592.022 kb on - strand, within MMJJ_RS03130at 592.023 kb on + strand, within MMJJ_RS03130at 592.030 kb on - strand, within MMJJ_RS03130at 592.031 kb on + strand, within MMJJ_RS03130at 592.064 kb on - strand, within MMJJ_RS03130at 592.065 kb on + strand, within MMJJ_RS03130at 592.067 kb on - strand, within MMJJ_RS03130at 592.067 kb on - strand, within MMJJ_RS03130at 592.072 kb on - strand, within MMJJ_RS03130at 592.079 kb on - strand, within MMJJ_RS03130at 592.079 kb on - strand, within MMJJ_RS03130at 592.142 kb on - strand, within MMJJ_RS03130at 592.142 kb on - strand, within MMJJ_RS03130at 592.142 kb on - strand, within MMJJ_RS03130at 592.143 kb on + strand, within MMJJ_RS03130at 592.146 kb on - strand, within MMJJ_RS03130at 592.147 kb on + strand, within MMJJ_RS03130at 592.150 kb on + strand, within MMJJ_RS03130at 592.150 kb on + strand, within MMJJ_RS03130at 592.162 kb on + strand, within MMJJ_RS03130at 592.213 kb on - strand, within MMJJ_RS03130at 592.214 kb on + strand, within MMJJ_RS03130at 592.214 kb on + strand, within MMJJ_RS03130at 592.230 kb on - strand, within MMJJ_RS03130at 592.230 kb on - strand, within MMJJ_RS03130at 592.231 kb on + strand, within MMJJ_RS03130at 592.231 kb on + strand, within MMJJ_RS03130at 592.241 kb on - strandat 592.255 kb on - strandat 592.255 kb on - strandat 592.255 kb on - strandat 592.256 kb on + strandat 592.256 kb on + strandat 592.326 kb on - strandat 592.327 kb on + strandat 592.329 kb on + strandat 592.365 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE
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589,226 - +0.5
589,227 + -1.1
589,230 - -0.3
589,241 - -0.4
589,244 - -1.6
589,244 - -1.8
589,244 - -1.6
589,244 - -2.6
589,245 + -1.1
589,272 + -0.8
589,485 + -0.8
589,485 + -0.8
589,844 - MMJJ_RS03120 0.71 -2.0
590,126 - -0.4
590,147 - -0.4
590,147 - -0.5
590,165 - +0.1
590,181 - -0.0
590,244 + -1.2
590,265 - -2.6
590,310 + -2.6
590,310 + -1.0
590,310 + -1.2
590,341 - MMJJ_RS03125 0.10 +0.8
590,468 - MMJJ_RS03125 0.21 -3.0
590,469 + -1.5
590,469 + MMJJ_RS03125 0.21 -1.2
590,470 - MMJJ_RS03125 0.21 -0.2
590,706 + -0.2
590,736 + MMJJ_RS03125 0.44 -2.8
590,763 - MMJJ_RS03125 0.46 -2.2
590,770 - MMJJ_RS03125 0.47 -1.5
590,834 - MMJJ_RS03125 0.53 -3.8
590,835 + MMJJ_RS03125 0.53 -2.7
590,838 + MMJJ_RS03125 0.53 -0.9
590,888 - MMJJ_RS03125 0.57 -3.4
591,283 - -0.2
591,342 - -2.1
591,439 - +0.1
591,439 - +0.2
591,440 + +0.1
591,482 + +0.4
591,580 - MMJJ_RS03130 0.19 +0.4
591,580 - MMJJ_RS03130 0.19 +0.8
591,760 - MMJJ_RS03130 0.38 +0.7
591,903 - MMJJ_RS03130 0.54 -1.7
591,985 - MMJJ_RS03130 0.63 +2.8
591,994 + MMJJ_RS03130 0.64 +0.4
592,022 - MMJJ_RS03130 0.67 -0.2
592,022 - MMJJ_RS03130 0.67 +0.5
592,022 - MMJJ_RS03130 0.67 +0.0
592,023 + MMJJ_RS03130 0.67 +0.3
592,030 - MMJJ_RS03130 0.68 -0.4
592,031 + MMJJ_RS03130 0.68 -0.1
592,064 - MMJJ_RS03130 0.71 -1.0
592,065 + MMJJ_RS03130 0.72 -0.1
592,067 - MMJJ_RS03130 0.72 +1.6
592,067 - MMJJ_RS03130 0.72 -0.1
592,072 - MMJJ_RS03130 0.72 +0.4
592,079 - MMJJ_RS03130 0.73 +0.2
592,079 - MMJJ_RS03130 0.73 -0.4
592,142 - MMJJ_RS03130 0.80 +0.8
592,142 - MMJJ_RS03130 0.80 -0.2
592,142 - MMJJ_RS03130 0.80 -0.6
592,143 + MMJJ_RS03130 0.80 -0.4
592,146 - MMJJ_RS03130 0.80 -0.0
592,147 + MMJJ_RS03130 0.80 +0.0
592,150 + MMJJ_RS03130 0.81 +0.4
592,150 + MMJJ_RS03130 0.81 -0.1
592,162 + MMJJ_RS03130 0.82 +0.0
592,213 - MMJJ_RS03130 0.88 -0.4
592,214 + MMJJ_RS03130 0.88 -1.0
592,214 + MMJJ_RS03130 0.88 -0.5
592,230 - MMJJ_RS03130 0.89 -0.9
592,230 - MMJJ_RS03130 0.89 +0.6
592,231 + MMJJ_RS03130 0.90 +0.3
592,231 + MMJJ_RS03130 0.90 +0.3
592,241 - -0.6
592,255 - +0.0
592,255 - +0.2
592,255 - -0.0
592,256 + -0.1
592,256 + +0.2
592,326 - +0.9
592,327 + -0.6
592,329 + -1.6
592,365 - +0.2

Or see this region's nucleotide sequence